FastQCFastQC Report
Wed 22 Feb 2023
EGAF00007699700

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007699700
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105187344
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTATG1550567014.741003442391321TruSeq Adapter, Index 19 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT23761562.2589751862163188TruSeq Adapter, Index 19 (97% over 38bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17643181.6773101524457164No Hit
GGCGTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCC13776281.309689880562057No Hit
CTCGGATCGGCCCCGCCGGGGTCGGCCCACGGCCCTGGCGGAGCGCTGAG11310441.0752662411554No Hit
GTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCCTTC6862990.6524539682264437No Hit
AGTATGGCCCGGGGGATCCGTATACGTTTCTAATTTGTAGTTAACGGTTG4655070.4425503889517355No Hit
AGTATGGCCCGGGGGATCCTAGAGACCATTCGCGATTCCATGAGACTCCA4322880.4109695934522313No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCAGAAGACCTAAGCTAGC4229180.40206167768624335No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAAT4087200.3885638561232233No Hit
NTCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTATG3934810.3740763717733951TruSeq Adapter, Index 12 (97% over 34bp)
AGTATGGCCCGGGGGATCCGTTAGCTATCGTTCGCGAGAAAGTTAGTAGA3928380.3734650815025808No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCGTCTTCAGAGGGGGATA3900350.3708003122504928No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTG3839570.36502205056152004No Hit
AGTATGGCCCGGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTA3831130.36421967266328165No Hit
AGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACC3717730.353438908011595No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCTCTAAACGTGGTAAATT3678520.34971127324975526No Hit
GGGGGATCCTAGAGACCATTCGCGATTCCATGAGACTCCAAGGGTTCTGC3632220.3453096030260066No Hit
GGGGGATCCGTTAGCTATCGTTCGCGAGAAAGTTAGTAGACACACAGGAC3380690.3213970304260178No Hit
AGTATGGCCCGGGGGATCCGCTCGCACTTAGCCTGTTAAGGGGTTCGCGC3369050.3202904334194426No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCGTCTTC3340830.31760760115779707No Hit
GCGTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCCT3200540.3042704453113675No Hit
GGGGGATCCGTATACGTTTCTAATTTGTAGTTAACGGTTGGATACCACTT3114200.29606223349455424No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTG3103760.2950697186536053No Hit
GGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTAAGACCGTCTG3078540.29267209180602566No Hit
GCCCTTTGTACACACCGCCCGTCGCTACTACCGATTGGATGGTTTAGTGA3076520.292480053493888No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCACGCCCATATAAACGAT2772550.26358209025602924No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACG2642320.2512013232314336No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCTCTAAA2638370.25082580276958033No Hit
GGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACCCTTTAAGAGT2569490.24427748646262992No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCAGAAGA2548440.2422762951406017No Hit
GATCCGTATACGTTTCTAATTTGTAGTTAACGGTTGGATACCACTTTGAG2514020.2390040383565536No Hit
ACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCCTTCTG2491230.23683742789436724No Hit
AGTATGGCCCGGGGGATCCTTGGACCGTTAATTCATATATCGAAGTAGCA2440140.23198037969282692No Hit
CCCGTCGCTACTACCGATTGGATGGTTTAGTGAGGCCCTCGGATCGGCCC2372540.22555375102921127No Hit
GGGGGATCCGCTCGCACTTAGCCTGTTAAGGGGTTCGCGCTCGTCTAGTC2345110.22294602285993648No Hit
CCTGCCCTTTGTACACACCGCCCGTCGCTACTACCGATTGGATGGTTTAG2307310.2193524346427076No Hit
GATCCGTTAGCTATCGTTCGCGAGAAAGTTAGTAGACACACAGGACCCAG2215810.21065366951370118No Hit
AGTATGGCCCGGGGGATCCTTAAGTCGTGTCCTTCTCCTACGATCTTGTG1996230.18977853457351296No Hit
CGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCCTTCTGA1980130.18824793218469324No Hit
CTAGTATGGCCCGGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTC1949240.18531126710452922No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTAACTCGTATG1944300.18484162885603422TruSeq Adapter, Index 19 (97% over 37bp)
CTACGTTCCAAATGCAGCGAGCTCGTATAACCCTTTAAGAGTTGCTCTTT1907860.18137733375984852No Hit
CTGCCCTTTGTACACACCGCCCGTCGCTACTACCGATTGGATGGTTTAGT1892060.1798752519124354No Hit
CTAGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAA1863920.17720002512849833No Hit
CTAGTATGGCCCGGGGGATCCTAGAGACCATTCGCGATTCCATGAGACTC1796380.170779100573164No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCGAGTCACTGTTAGAAGT1756500.1669877699355162No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCACGCCC1658440.15766535563441927No Hit
GTTTAGTGAGGCCCTCGGATCGGCCCCGCCGGGGTCGGCCCACGGCCCTG1608080.15287770741696835No Hit
GTCGCTACTACCGATTGGATGGTTTAGTGAGGCCCTCGGATCGGCCCCGC1595550.15168649947088692No Hit
GATCCGCTCGCACTTAGCCTGTTAAGGGGTTCGCGCTCGTCTAGTCTGTG1541730.14656991434254676No Hit
CTTCGTCCTGGGAAACGGGGCGCGGCCGGAAAGGCGGCCGCCCCCTCGCC1489240.1415797702811091No Hit
CTAGTATGGCCCGGGGGATCCGTATACGTTTCTAATTTGTAGTTAACGGT1469660.13971832961197309No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCACGGTCATCAATTCTTT1466770.13944358172975638No Hit
CCAGTAAGTGCGGGTCATAAGCTTGCGTTGATTAAGTCCCTGCCCTTTGT1422650.13524916077356225No Hit
GCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAGGGTTATAAGCTGG1331390.12657321207768113No Hit
GGGGGATCCTTGGACCGTTAATTCATATATCGAAGTAGCAGGTTGTTGCC1324140.12588396566035548No Hit
GCAGGATATCGCGGCCGCGTCTTCAGAGGGGGATAGCATGACCTCACGCC1321850.12566625886095195No Hit
CGTTGATTAAGTCCCTGCCCTTTGTACACACCGCCCGTCGCTACTACCGA1292010.12282941567571094No Hit
GCCCTTCGTCCTGGGAAACGGGGCGCGGCCGGAAAGGCGGCCGCCCCCTC1267310.12048122443323599No Hit
GCGGGTCATAAGCTTGCGTTGATTAAGTCCCTGCCCTTTGTACACACCGC1201520.11422666970277338No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGCCCGTGGACGGTGTGAGG1193030.11341953838096719No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTACCTCGTATG1181810.11235287013236117TruSeq Adapter, Index 19 (97% over 37bp)
TACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCCTTCT1154170.10972517758410176No Hit
CTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAGGGTTATAAGCT1139970.10837520529085705No Hit
CTTATCTGTCAAAACCGCTAATGTCCGTTCTAAGACCGTCTGGAGAACAC1137120.10810426014749455No Hit
GATCCTTGGACCGTTAATTCATATATCGAAGTAGCAGGTTGTTGCCCCGC1131220.10754335616649852No Hit
CTAGAGACCATTCGCGATTCCATGAGACTCCAAGGGTTCTGCACAACTTA1129700.10739885209003852No Hit
GGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCTCTAAAC1106800.10522178409600304No Hit
CCCGGCGCCGCTCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCAGCCC1082360.10289831065608045No Hit
GGGGGATCCTTAAGTCGTGTCCTTCTCCTACGATCTTGTGAACGATGGAT1052370.10004720720013617No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)