FastQCFastQC Report
Wed 22 Feb 2023
EGAF00007699772

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007699772
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89674248
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGGATCGGCCCCGCCGGGGTCGGCCCACGGCCCTGGCGGAGCGCTGAG10981991.2246537043722965No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTATG10459661.166406212851654TruSeq Adapter, Index 8 (97% over 35bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9376091.0455721914724059No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTAT4126830.46020235374597174TruSeq Adapter, Index 8 (97% over 36bp)
CTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAATCTAAAACTTCGAT2000080.2230383911332047No Hit
CTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAGGGTTATAAGCT1915760.21363546867992694No Hit
CTACGTTCCAAATGCAGCGAGCTCGTATAACCCTTTAAGAGTTGCTCTTT1913560.2133901362629771No Hit
CTGCAGGATATCGCGGCCGCGTCTTCAGAGGGGGATAGCATGACCTCACG1771830.19758515287465805No Hit
AGTATGGCCCGGGGGATCCTAGAGACCATTCGCGATTCCATGAGACTCCA1729900.19290934003706392No Hit
AGTATGGCCCGGGGGATCCGTATACGTTTCTAATTTGTAGTTAACGGTTG1686690.18809078833869897No Hit
GATCCGTATACGTTTCTAATTTGTAGTTAACGGTTGGATACCACTTTGAG1649910.18398927638623744No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCGTCTTCAGAGGGGGATA1639040.18277711121703524No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCAGAAGACCTAAGCTAGC1626700.18140101938741654No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTG1587730.17705529016535496No Hit
GGCGTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCC1584800.17672855199187174No Hit
AGTATGGCCCGGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTA1549740.172818845383571No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAAT1530730.17069895027165435No Hit
AGTATGGCCCGGGGGATCCGTTAGCTATCGTTCGCGAGAAAGTTAGTAGA1497400.16698216415486417No Hit
CCGGCGGGGCGTGCGCGTCCGGCGCCGTCCGTCCTTCCGTTCGTCTTCCT1476240.16462251236274655No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCTCTAAACGTGGTAAATT1456330.16240225398935043No Hit
GATCCGTTAGCTATCGTTCGCGAGAAAGTTAGTAGACACACAGGACCCAG1455310.16228850895967367No Hit
GTTTAGTGAGGCCCTCGGATCGGCCCCGCCGGGGTCGGCCCACGGCCCTG1387840.1547646097907618No Hit
AGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACC1364170.15212505601385137No Hit
CGGCGGGGCGTGCGCGTCCGGCGCCGTCCGTCCTTCCGTTCGTCTTCCTC1274190.14209096016060263No Hit
CCCGTCGCTACTACCGATTGGATGGTTTAGTGAGGCCCTCGGATCGGCCC1257750.1402576579175774No Hit
GGGGGATCCTAGAGACCATTCGCGATTCCATGAGACTCCAAGGGTTCTGC1256250.1400903858151116No Hit
CCCGCTTCTTCGGTTCCCGCCTCCTCCCCGTTCACCGCCGGGGCGGCTCG1193980.13314636326808116No Hit
AGTATGGCCCGGGGGATCCGCTCGCACTTAGCCTGTTAAGGGGTTCGCGC1190280.1327337587486655No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCGTCTTC1184070.13204125224445706No Hit
GTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCCTTC1121860.12510392058152525No Hit
CCCGGCGGGGCGTGCGCGTCCGGCGCCGTCCGTCCTTCCGTTCGTCTTCC1090320.12158674584034426No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCACGCCCATATAAACGAT1070080.11932968760440567No Hit
GGGGGATCCGTTAGCTATCGTTCGCGAGAAAGTTAGTAGACACACAGGAC1043450.11636005021196275No Hit
CTGCAGGATATCGCGGCCGCAGAAGACCTAAGCTAGCGTTTAGAGTGCAA1008590.11247264655065745No Hit
GCCCTTTGTACACACCGCCCGTCGCTACTACCGATTGGATGGTTTAGTGA1005320.11210799336728199No Hit
CTGCAGGATATCGCGGCCGCTCTAAACGTGGTAAATTGTTAACTCCGAGC994200.1108679495143355No Hit
CTAGTATGGCCCGGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTC990570.11046315102636825No Hit
CTTATCTGTCAAAACCGCTAATGTCCGTTCTAAGACCGTCTGGAGAACAC988600.11024346699846316No Hit
CTAGAGACCATTCGCGATTCCATGAGACTCCAAGGGTTCTGCACAACTTA987200.11008734636949505No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTG976810.10892870827308192No Hit
CTAGTATGGCCCGGGGGATCCTAGAGACCATTCGCGATTCCATGAGACTC954140.10640066923114871No Hit
GGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTAAGACCGTCTG942330.10508368021106797No Hit
GGGGGATCCGTATACGTTTCTAATTTGTAGTTAACGGTTGGATACCACTT911100.1016010750377299No Hit
GGATGGTTTAGTGAGGCCCTCGGATCGGCCCCGCCGGGGTCGGCCCACGG907240.1011706281607179No Hit
CTAGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAA906850.10112713741407678No Hit
GATCCGCTCGCACTTAGCCTGTTAAGGGGTTCGCGCTCGTCTAGTCTGTG902050.10059186668618621No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)