FastQCFastQC Report
Wed 22 Feb 2023
EGAF00007699773

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007699773
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89674248
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTC10663771.1891674854078509Illumina Single End PCR Primer 1 (96% over 33bp)
CGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCG7112140.7931084072207665No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT4168830.46488597261501424Illumina Single End PCR Primer 1 (97% over 34bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3303140.36834878169259916No Hit
CCTTCCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCT3135450.349648875784272No Hit
CTTCCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCTA2229170.2485853017691322No Hit
CTACGTTCCAAATGCAGCGAGCTCGTATAACCCTTTAAGAGTTGCTCTTT1988100.2217024446081778No Hit
CTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAATCTAAAACTTCGAT1954800.21798900393343695No Hit
CTGCAGGATATCGCGGCCGCGTCTTCAGAGGGGGATAGCATGACCTCACG1949790.21743031511120117No Hit
CTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAGGGTTATAAGCT1838220.20498861612979458No Hit
CTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTC1779100.19839586499794232No Hit
AGTATGGCCCGGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTA1575780.17572268908237737No Hit
AGTATGGCCCGGGGGATCCTAGAGACCATTCGCGATTCCATGAGACTCCA1567970.1748517590022054No Hit
AGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACC1541480.1718977336726593No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCGTCTTCAGAGGGGGATA1529720.17058632038932736No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCAGAAGACCTAAGCTAGC1493250.16651937800470878No Hit
AGTATGGCCCGGGGGATCCGTATACGTTTCTAATTTGTAGTTAACGGTTG1438660.16043178862230326No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTG1435780.1601106261855689No Hit
CGGCGCGCCGGGGGCCGCTACCGGCCTCACACCGTCCACGGGCTGGGCCT1408780.1570997283411844No Hit
GTTGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1397420.1558329209518434No Hit
CTGCAGGATATCGCGGCCGCAGAAGACCTAAGCTAGCGTTTAGAGTGCAA1353460.15093073320224554No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAAT1318800.14706563248793567No Hit
AGTATGGCCCGGGGGATCCGTTAGCTATCGTTCGCGAGAAAGTTAGTAGA1258590.14035133029495828No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCTCTAAACGTGGTAAATT1222440.13632007262553236No Hit
GGGGGATCCTAGAGACCATTCGCGATTCCATGAGACTCCAAGGGTTCTGC1214990.1354892878499522No Hit
CTGCAGGATATCGCGGCCGCTCTAAACGTGGTAAATTGTTAACTCCGAGC1090250.12157893980889586No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCGTCTTC1085510.12105035996510392No Hit
CCGGCGGGGCGTGCGCGTCCGGCGCCGTCCGTCCTTCCGTTCGTCTTCCT1083000.12077045798031114No Hit
CGGCGGGGCGTGCGCGTCCGGCGCCGTCCGTCCTTCCGTTCGTCTTCCTC1080640.12050728320576493No Hit
CTTATCTGTCAAAACCGCTAATGTCCGTTCTAAGACCGTCTGGAGAACAC1041350.11612586926851062No Hit
GGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTAAGACCGTCTG971190.10830199546251004No Hit
CCTACGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCG959470.10699504276857723No Hit
GGGGGATCCGTTAGCTATCGTTCGCGAGAAAGTTAGTAGACACACAGGAC955960.10660362604880723No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCGTCTTCAGAGGGGGATAGC955240.10652333543962364No Hit
CCCACCACCACGCCCGCGGTCGGCGGGAGAGGCCGGGAGGGAGGAAGACG950340.10597691323823534No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAG944300.10530336423897305No Hit
GATCCGTATACGTTTCTAATTTGTAGTTAACGGTTGGATACCACTTTGAG939950.10481827514182221No Hit
GGGGGATCCGTATACGTTTCTAATTTGTAGTTAACGGTTGGATACCACTT901700.10055283652894419No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTG899540.10031196470139342No Hit
GATCCGTTAGCTATCGTTCGCGAGAAAGTTAGTAGACACACAGGACCCAG897990.10013911686217877No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)