FastQCFastQC Report
Wed 22 Feb 2023
EGAF00007699814

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007699814
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88242780
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3297790.37371782711288104No Hit
CTCGGATCGGCCCCGCCGGGGTCGGCCCACGGCCCTGGCGGAGCGCTGAG3287930.3726004552440438No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTATG2431160.2755080925600939TruSeq Adapter, Index 6 (97% over 35bp)
GGCGTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCC2147170.24332528961576233No Hit
GTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCCTTC1470610.1666549943236149No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT1170540.13264994597858318TruSeq Adapter, Index 6 (97% over 36bp)
CCGGCGGGGCGTGCGCGTCCGGCGCCGTCCGTCCTTCCGTTCGTCTTCCT951060.10777765614365278No Hit
GGGGGCCCGGCGGGGCGTGCGCGTCCGGCGCCGTCCGTCCTTCCGTTCGT887220.10054306992594748No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)