FastQCFastQC Report
Wed 22 Feb 2023
EGAF00007699892

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007699892
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81564876
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTATG2302180.28225139458312914TruSeq Adapter, Index 7 (97% over 34bp)
GGCGTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCC1652200.20256268151501877No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT1450240.1778020235082562TruSeq Adapter, Index 7 (97% over 35bp)
CTCGGATCGGCCCCGCCGGGGTCGGCCCACGGCCCTGGCGGAGCGCTGAG1299080.15926953655884918No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1280410.15698056109347852No Hit
GTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCCTTC1036360.12705959364175334No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)