FastQCFastQC Report
Wed 22 Feb 2023
EGAF00007699899

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007699899
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70493227
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCTC7521601.0669961243226955Illumina Single End PCR Primer 1 (96% over 32bp)
CCGGGCCCGGCGCGCCGGGGGCCGCTACCGGCCTCACACCGTCCACGGGC4560490.6469401663226454No Hit
CGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCG4043960.5736664601834727No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCT3586000.5087013536775667Illumina Single End PCR Primer 1 (96% over 33bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2696450.3825119255783254No Hit
CCTTCCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCT2066760.293185613420705No Hit
CGGCGCGCCGGGGGCCGCTACCGGCCTCACACCGTCCACGGGCTGGGCCT1444540.20491897753524604No Hit
CTTCCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCTA1390580.1972643414380789No Hit
CCCGGGCCCGGCGCGCCGGGGGCCGCTACCGGCCTCACACCGTCCACGGG1238220.17565091749878325No Hit
CTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTC1086030.15406160935149132No Hit
CTGCAGGATATCGCGGCCGCGTCTTCAGAGGGGGATAGCATGACCTCACG1084220.15380484709545217No Hit
CTACGTTCCAAATGCAGCGAGCTCGTATAACCCTTTAAGAGTTGCTCTTT1083480.15369987247143616No Hit
CTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAGGGTTATAAGCT1071120.1519465125351688No Hit
CTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAATCTAAAACTTCGAT1032000.1463970432223226No Hit
CCCACCACCACGCCCGCGGTCGGCGGGAGAGGCCGGGAGGGAGGAAGACG1002570.14222217405368603No Hit
GTTGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT952460.13511368971660215No Hit
AGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACC950630.13485409030856255No Hit
AGTATGGCCCGGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTA914470.1297245194917804No Hit
GGGGGATCCGTTAGCTATCGTTCGCGAGAAAGTTAGTAGACACACAGGAC911680.12932873678772006No Hit
AGTATGGCCCGGGGGATCCTAGAGACCATTCGCGATTCCATGAGACTCCA889630.12620077670724308No Hit
AGTATGGCCCGGGGGATCCGTTAGCTATCGTTCGCGAGAAAGTTAGTAGA878890.1246772260830108No Hit
AGTATGGCCCGGGGGATCCGTATACGTTTCTAATTTGTAGTTAACGGTTG869480.12334234606680725No Hit
CTCCGCGGCTCCCAAACCACGCTCCCCGGACCCCGTCCCGGCCCGGAGCG860710.12209825491461754No Hit
GGGGGATCCTAGAGACCATTCGCGATTCCATGAGACTCCAAGGGTTCTGC847760.12026119899433743No Hit
CCCGACTCCGGGAAGACCCGGGCCCGGCGCGCCGGGGGCCGCTACCGGCC847090.12016615440232294No Hit
CTGCAGGATATCGCGGCCGCAGAAGACCTAAGCTAGCGTTTAGAGTGCAA829260.11763683339393725No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCAGAAGACCTAAGCTAGC825390.11708784448185355No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCGTCTTCAGAGGGGGATA824690.1169885441618384No Hit
CGGGCCCGGCGCGCCGGGGGCCGCTACCGGCCTCACACCGTCCACGGGCT818520.11611328276970496No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTG801150.11364921625732922No Hit
GGGGGCCCGGCGGGGCGTGCGCGTCCGGCGCCGTCCGTCCTTCCGTTCGT786000.11150007361700152No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAAT784920.11134686740897817No Hit
CTGCAGGATATCGCGGCCGCTCTAAACGTGGTAAATTGTTAACTCCGAGC777320.11026874964881377No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCGTCTTC771680.10946867278469179No Hit
GGGGGATCCGTATACGTTTCTAATTTGTAGTTAACGGTTGGATACCACTT767750.10891117241660679No Hit
CGGGAAGACCCGGGCCCGGCGCGCCGGGGGCCGCTACCGGCCTCACACCG737720.10465118868795721No Hit
CCCACCCCCACCACCACGCCCGCGGTCGGCGGGAGAGGCCGGGAGGGAGG737370.10460153852794964No Hit
AGTATGGCCCGGGGGATCCGCTCGCACTTAGCCTGTTAAGGGGTTCGCGC732300.10388232049583998No Hit
CCGGCGGGGCGTGCGCGTCCGGCGCCGTCCGTCCTTCCGTTCGTCTTCCT718450.10191759273554041No Hit
GGGGGATCCGCTCGCACTTAGCCTGTTAAGGGGTTCGCGCTCGTCTAGTC718290.10189489551953694No Hit
GGAAGACCCGGGCCCGGCGCGCCGGGGGCCGCTACCGGCCTCACACCGTC705680.10010607118326417No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)