FastQCFastQC Report
Wed 22 Feb 2023
EGAF00007699905

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007699905
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108292982
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCTC5891150.5440010877159149Illumina Single End PCR Primer 1 (96% over 33bp)
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA1581740.14606117319772394No Hit
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT1486600.13727574701008785No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT1336010.12336995208055127Illumina Single End PCR Primer 1 (97% over 34bp)
GTCGGGTTCAGATCCCCGAATCCGGAGTGGCGGAGATGGGCGCCGCGAGG1165320.10760808119588026No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)