FastQCFastQC Report
Wed 22 Feb 2023
EGAF00007700047

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007700047
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68827955
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCTC4593130.6673349513290058Illumina Single End PCR Primer 1 (96% over 32bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA910520.13228927112537342No Hit
CCGGGCCCGGCGCGCCGGGGGCCGCTACCGGCCTCACACCGTCCACGGGC853760.12404262192593112No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCT691700.10049695650553615Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)