FastQCFastQC Report
Wed 22 Feb 2023
EGAF00007700060

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007700060
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88967421
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTATG7194090.8086207197126688TruSeq Adapter, Index 6 (97% over 35bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4324390.4860644437473354No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT2154530.24217067054242247TruSeq Adapter, Index 6 (97% over 36bp)
AGTATGGCCCGGGGGATCCTTGGACCGTTAATTCATATATCGAAGTAGCA1037140.11657525736302955No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCTCTAAACGTGGTAAATT941220.10579378264769528No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAAT896380.10075373546008488No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)