FastQCFastQC Report
Wed 22 Feb 2023
EGAF00007700080

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007700080
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63698963
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTATG25507724.004416837994678TruSeq Adapter, Index 1 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT19673573.0885228068783475TruSeq Adapter, Index 1 (97% over 36bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7474031.1733362127104014No Hit
AGTATGGCCCGGGGGATCCGCTCGCACTTAGCCTGTTAAGGGGTTCGCGC1987000.3119360043585011No Hit
AGTATGGCCCGGGGGATCCTTGGACCGTTAATTCATATATCGAAGTAGCA1961030.30785901491049394No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCTCTAAACGTGGTAAATT1859820.29197021621843355No Hit
AGTATGGCCCGGGGGATCCTTAAGTCGTGTCCTTCTCCTACGATCTTGTG1847440.2900266994927374No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCACGCCCATATAAACGAT1840310.2889073720085522No Hit
AGTATGGCCCGGGGGATCCGTTAGCTATCGTTCGCGAGAAAGTTAGTAGA1737820.2728176281299901No Hit
AGTATGGCCCGGGGGATCCGTATACGTTTCTAATTTGTAGTTAACGGTTG1732650.2720059979626356No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCAGAAGACCTAAGCTAGC1644200.2581203715985141No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCGAGTCACTGTTAGAAGT1573700.24705268749822507No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAAT1562390.24527714838937018No Hit
AGTATGGCCCGGGGGATCCTAGAGACCATTCGCGATTCCATGAGACTCCA1521280.23882335415727254No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTG1468670.23056419301519868No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCGTCTTCAGAGGGGGATA1455380.22847781682097398No Hit
GATCCGCTCGCACTTAGCCTGTTAAGGGGTTCGCGCTCGTCTAGTCTGTG1397320.21936306875199835No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCACGGTCATCAATTCTTT1386160.21761107790718667No Hit
AGTATGGCCCGGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTA1353510.2124854057671237No Hit
AGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACC1300510.20416501913853763No Hit
GATCCTTGGACCGTTAATTCATATATCGAAGTAGCAGGTTGTTGCCCCGC1249850.19621198542902496No Hit
GATCCGTTAGCTATCGTTCGCGAGAAAGTTAGTAGACACACAGGACCCAG1246490.195684504314458No Hit
GGGGGATCCGCTCGCACTTAGCCTGTTAAGGGGTTCGCGCTCGTCTAGTC1181060.18541275153882802No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCACGCCC1100380.17274692525214264No Hit
GGGGGATCCGTTAGCTATCGTTCGCGAGAAAGTTAGTAGACACACAGGAC1070220.1680121542951963No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCTCTAAA1025870.16104971755976624No Hit
GGGGGATCCTTGGACCGTTAATTCATATATCGAAGTAGCAGGTTGTTGCC1025340.16096651369348036No Hit
GATCCGTATACGTTTCTAATTTGTAGTTAACGGTTGGATACCACTTTGAG996730.15647507479831343No Hit
GATCCTTAAGTCGTGTCCTTCTCCTACGATCTTGTGAACGATGGATATTT982090.15417676422770024No Hit
GGGGGATCCTTAAGTCGTGTCCTTCTCCTACGATCTTGTGAACGATGGAT978640.15363515415470735No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCGAGTCA941490.14780303409334936No Hit
CCTCTCCCCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCG889180.13959096947936186No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCACGGTC871370.136795005595303No Hit
GGGGGATCCGGCGCGCCTAGGCCTTGACGGCCTTCACTCAATTCGCCCTA704300.11056694910402232No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCAGAAGA667600.10480547383479383No Hit
CTAGTATGGCCCGGGGGATCCGCTCGCACTTAGCCTGTTAAGGGGTTCGC662610.1040221015842911No Hit
CTAGTATGGCCCGGGGGATCCTAGAGACCATTCGCGATTCCATGAGACTC656570.10307389148548621No Hit
GGGGGATCCTAGAGACCATTCGCGATTCCATGAGACTCCAAGGGTTCTGC650820.10217120803049806No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)