FastQCFastQC Report
Wed 22 Feb 2023
EGAF00007700108

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007700108
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66787999
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTATG15743242.357195938749415TruSeq Adapter, Index 6 (97% over 35bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6575070.9844687815845479No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT3838670.5747544555122844TruSeq Adapter, Index 6 (97% over 36bp)
GTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAAT1914080.2865904097531055No Hit
AGTATGGCCCGGGGGATCCGTATACGTTTCTAATTTGTAGTTAACGGTTG1905860.28535964971790817No Hit
AGTATGGCCCGGGGGATCCTAGAGACCATTCGCGATTCCATGAGACTCCA1885940.2823770779537803No Hit
AGTATGGCCCGGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTA1876170.2809142402963742No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTG1813660.2715547743839428No Hit
AGTATGGCCCGGGGGATCCGTTAGCTATCGTTCGCGAGAAAGTTAGTAGA1767390.26462688304226634No Hit
AGTATGGCCCGGGGGATCCGCTCGCACTTAGCCTGTTAAGGGGTTCGCGC1751730.26228215042046704No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCGTCTTCAGAGGGGGATA1636270.24499461347838852No Hit
AGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACC1626150.24347937119661273No Hit
AGTATGGCCCGGGGGATCCTTGGACCGTTAATTCATATATCGAAGTAGCA1572970.23551686284237983No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCAGAAGACCTAAGCTAGC1570500.23514703592182779No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCTCTAAACGTGGTAAATT1421300.21280769319050866No Hit
AGTATGGCCCGGGGGATCCTTAAGTCGTGTCCTTCTCCTACGATCTTGTG1387910.20780829202563775No Hit
GGGGGATCCTAGAGACCATTCGCGATTCCATGAGACTCCAAGGGTTCTGC1321220.19782296517073375No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTG1264120.18927352502356No Hit
GGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTAAGACCGTCTG1211320.18136791311864278No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCGTCTTC1141700.17094388469401517No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCACGCCCATATAAACGAT1115340.16699706784148452No Hit
GGGGGATCCGTTAGCTATCGTTCGCGAGAAAGTTAGTAGACACACAGGAC1050120.15723184040893334No Hit
GGGGGATCCGTATACGTTTCTAATTTGTAGTTAACGGTTGGATACCACTT986660.14773013337321275No Hit
GGGGGATCCGCTCGCACTTAGCCTGTTAAGGGGTTCGCGCTCGTCTAGTC948980.14208840124106728No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCGAGTCACTGTTAGAAGT923510.13827484186193392No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCTCTAAA904780.1354704458206631No Hit
GATCCGTATACGTTTCTAATTTGTAGTTAACGGTTGGATACCACTTTGAG888290.13300143937535844No Hit
GATCCGTTAGCTATCGTTCGCGAGAAAGTTAGTAGACACACAGGACCCAG835830.12514673481982894No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCAGAAGA817350.12237977065310791No Hit
CTAGTATGGCCCGGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTC815020.1220309055823038No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACG798830.1196068173864589No Hit
GGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACCCTTTAAGAGT787180.11786249203243834No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCACGGTCATCAATTCTTT770660.11538899376218772No Hit
GCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAGGGTTATAAGCTGG755580.11313110308934393No Hit
GATCCGCTCGCACTTAGCCTGTTAAGGGGTTCGCGCTCGTCTAGTCTGTG750230.11233006097397828No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCACGCCC731780.10956758863220323No Hit
CTAGTATGGCCCGGGGGATCCTAGAGACCATTCGCGATTCCATGAGACTC722270.10814368012432893No Hit
GCAGGATATCGCGGCCGCGTCTTCAGAGGGGGATAGCATGACCTCACGCC719240.10769000580478538No Hit
GGGGGATCCTTGGACCGTTAATTCATATATCGAAGTAGCAGGTTGTTGCC710460.1063753983705965No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)