FastQCFastQC Report
Wed 15 May 2024
EGAF00007700147

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007700147
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73008298
Total Bases5.5 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCTC7282720.9975194874423726Illumina Single End Sequencing Primer (100% over 31bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCT2207430.30235330236023306Illumina Single End Sequencing Primer (100% over 32bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA982660.13459565924958283No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)