FastQCFastQC Report
Wed 22 Feb 2023
EGAF00007700161

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007700161
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52945413
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCTC625670611.81727678656506Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT15524342.9321406936612244Illumina Single End PCR Primer 1 (96% over 32bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6211201.1731327886704745No Hit
AGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACC4358910.8232837847539315No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAAT3781250.714178960130125No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTG3486390.6584876389575052No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCGTCTTCAGAGGGGGATA3382350.6388372114502158No Hit
AGTATGGCCCGGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTA3322460.6275255610906275No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCAGAAGACCTAAGCTAGC3193470.6031627329075703No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAG3057090.5774041275303678No Hit
CCGGGCCCGGCGCGCCGGGGGCCGCTACCGGCCTCACACCGTCCACGGGC2941540.5555797628776642No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCGTCTTCAGAGGGGGATAGC2811890.5310922780033843No Hit
GGGGGATCCTAGAGACCATTCGCGATTCCATGAGACTCCAAGGGTTCTGC2803880.5295793990689996No Hit
GGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTAAGACCGTCTG2718240.5134042490139797No Hit
AGTATGGCCCGGGGGATCCTAGAGACCATTCGCGATTCCATGAGACTCCA2613330.4935895013228058No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCGTCTTC2495330.47130239592238143No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCTCTAAACGTGGTAAATT2468770.4662859084695401No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTG2433900.4596998799499401No Hit
CGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCG2306520.4356411385439566No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCACGCCCATATAAACGAT2271280.42898522672776207No Hit
AGTATGGCCCGGGGGATCCGTATACGTTTCTAATTTGTAGTTAACGGTTG2131960.40267133245329484No Hit
GGGGGATCCGTTAGCTATCGTTCGCGAGAAAGTTAGTAGACACACAGGAC2059280.3889439865168301No Hit
GGGGGATCCGTATACGTTTCTAATTTGTAGTTAACGGTTGGATACCACTT2046930.3866113954007687No Hit
GGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACCCTTTAAGAGT2010460.37972316884184093No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCGAGTCACTGTTAGAAGT1948680.3680545470482967No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACG1808810.34163677219781063No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCAGAAGACCTAAGCTAGCGT1755710.3316075747676196No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCAGAAGA1696830.320486686920357No Hit
GCAGGATATCGCGGCCGCGTCTTCAGAGGGGGATAGCATGACCTCACGCC1666910.31483558358492736No Hit
AGTATGGCCCGGGGGATCCGTTAGCTATCGTTCGCGAGAAAGTTAGTAGA1652820.312174352101097No Hit
GGGGGATCCGCTCGCACTTAGCCTGTTAAGGGGTTCGCGCTCGTCTAGTC1641900.31011185048268486No Hit
GCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1581430.29869065333384026No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCTCTAAA1573620.29721554915437154No Hit
GCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAGGGTTATAAGCTGG1519830.2870560288197204No Hit
GATCCGTTAGCTATCGTTCGCGAGAAAGTTAGTAGACACACAGGACCCAG1464280.27656409064180876No Hit
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGAC1437950.2715910441571209No Hit
GATCCGTATACGTTTCTAATTTGTAGTTAACGGTTGGATACCACTTTGAG1398630.26416452734064044No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCACGGTCATCAATTCTTT1298830.24531492463756963No Hit
CTTATCTGTCAAAACCGCTAATGTCCGTTCTAAGACCGTCTGGAGAACAC1237430.23371807487836577No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCACGCCC1236690.23357830828517664No Hit
CCTTCCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCT1207970.22815385347924289No Hit
AGTATGGCCCGGGGGATCCGCTCGCACTTAGCCTGTTAAGGGGTTCGCGC1146850.21660988837692136No Hit
GATCCGCTCGCACTTAGCCTGTTAAGGGGTTCGCGCTCGTCTAGTCTGTG1146780.21659666721270074No Hit
GTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACC1134940.2143604017216751No Hit
GCAGGATATCGCGGCCGCAGAAGACCTAAGCTAGCGTTTAGAGTGCAATT1117970.21115521376705476No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCTCTAAACGTGGTAAATTGT1104290.20857142053080216No Hit
CTGCAGGATATCGCGGCCGCGTCTTCAGAGGGGGATAGCATGACCTCACG1092860.20641259328735428No Hit
CTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAGGGTTATAAGCT1089390.20575720128956212No Hit
CTAGAGACCATTCGCGATTCCATGAGACTCCAAGGGTTCTGCACAACTTA1076130.2032527350386331No Hit
GTTGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1066050.201348887390868No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCACGCCCATATAAACGATCG1006980.19019211352643525No Hit
GCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGC985950.1862200980470206No Hit
GGGGGATCCTTGGACCGTTAATTCATATATCGAAGTAGCAGGTTGTTGCC985170.1860727765028483No Hit
GCAGGATATCGCGGCCGCTCTAAACGTGGTAAATTGTTAACTCCGAGCTA971180.18343043239647597No Hit
GAGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT970340.18327177842582887No Hit
GGGGGATCCTTAAGTCGTGTCCTTCTCCTACGATCTTGTGAACGATGGAT919630.1736939893168838No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT896150.1692592330897485No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCGAGTCA884210.16700408022126487No Hit
CGGGTTGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT883450.16686053615258417No Hit
CTTCCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCTA866740.16370445537935457No Hit
CGAGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT856960.16185726986396348No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT845070.15961156068420884No Hit
CCCGACTCCGGGAAGACCCGGGCCCGGCGCGCCGGGGGCCGCTACCGGCC842130.15905627178694404No Hit
CTGCAGGATATCGCGGCCGCAGAAGACCTAAGCTAGCGTTTAGAGTGCAA813250.15360159717707744No Hit
TGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAGGGTTATAAGCTG796290.15039829796020288No Hit
GCTGCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGCCCGGCGCGCC790610.14932549492058925No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCGAGTCACTGTTAGAAGTCC778610.14705900962563084No Hit
CGGCGCGCCGGGGGCCGCTACCGGCCTCACACCGTCCACGGGCTGGGCCT775280.14643005995627986No Hit
TGCAGGATATCGCGGCCGCGTCTTCAGAGGGGGATAGCATGACCTCACGC774280.1462411861817No Hit
GCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGG762580.14403136301911554No Hit
GATCCTTGGACCGTTAATTCATATATCGAAGTAGCAGGTTGTTGCCCCGC747630.14120770008914654No Hit
CGGGAAGACCCGGGCCCGGCGCGCCGGGGGCCGCTACCGGCCTCACACCG746740.14103960242977046No Hit
GGGGGATCCGGCGCGCCTAGGCCTTGACGGCCTTCACTCAATTCGCCCTA727240.13735656382546305No Hit
GCAGGATATCGCGGCCGCACGCCCATATAAACGATCGGATAAATAGCTGA719790.13594945420484303No Hit
AGTATGGCCCGGGGGATCCTTGGACCGTTAATTCATATATCGAAGTAGCA714640.1349767542657567No Hit
CCGGGTTGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT675100.1275086852188687No Hit
GGGCCATACTAGTACTGGATGCATCTGCAGGATATCGCGGCCGCACGGTC643620.12156293879509447No Hit
CTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAGG627220.11846540889198466No Hit
TGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCG626460.11832186482330395No Hit
GGAAGACCCGGGCCCGGCGCGCCGGGGGCCGCTACCGGCCTCACACCGTC621440.11737371847491303No Hit
CTGCAGGATATCGCGGCCGCTCTAAACGTGGTAAATTGTTAACTCCGAGC611630.11552086674628452No Hit
GTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGACTCCGGGAAG605640.11438951283655112No Hit
GCATTCCCAAGCAACCCGACTCCGGGAAGACCCGGGCCCGGCGCGCCGGG604960.1142610786698368No Hit
CTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTC582630.11004352728346835No Hit
CGGGCCCGGCGCGCCGGGGGCCGCTACCGGCCTCACACCGTCCACGGGCT575470.1086911910574765No Hit
CGGCGGGGCGTGCGCGTCCGGCGCCGTCCGTCCTTCCGTTCGTCTTCCTC566090.10691955505191734No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCACGGTCATCAATTCTTTAA558440.10547467067638136No Hit
AGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT555030.104830611105064No Hit
GATCCTTAAGTCGTGTCCTTCTCCTACGATCTTGTGAACGATGGATATTT547840.10347260866583476No Hit
CCTAGAGACCATTCGCGATTCCATGAGACTCCAAGGGTTCTGCACAACTT543350.10262456541797116No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)