FastQCFastQC Report
Wed 22 Feb 2023
EGAF00007700189

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007700189
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70985670
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCTC32298644.550022560891516Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTAAGATTAGTGTAGATCT4821870.6792737182025612Illumina Single End PCR Primer 1 (96% over 32bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1627040.22920682441963286No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCTCGACGTAGAACTCAAT1364140.192171180465015No Hit
AGTATGGCCCGGGGGATCCTACGTTCCAAATGCAGCGAGCTCGTATAACC1328380.1871335439955698No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTG1262240.17781617050314522No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCGTCTTCAGAGGGGGATA1243270.17514380015008663No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCAGAAGACCTAAGCTAGC1181380.16642513904566936No Hit
AGTATGGCCCGGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTA953120.13426935323706884No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCCATCTGCCCTACGTTTGAG925000.13030799033100623No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCTCTAAACGTGGTAAATT905800.12760321907224373No Hit
ACTGGATGCATCTGCAGGATATCGCGGCCGCGTCTTCAGAGGGGGATAGC874000.12312344167491832No Hit
GTACTGGATGCATCTGCAGGATATCGCGGCCGCACGCCCATATAAACGAT811900.11437519713485836No Hit
GGGGGATCCTAGAGACCATTCGCGATTCCATGAGACTCCAAGGGTTCTGC778550.10967706580778909No Hit
CCGGGCCCGGCGCGCCGGGGGCCGCTACCGGCCTCACACCGTCCACGGGC769010.10833313258859147No Hit
GGGGGATCCTTATCTGTCAAAACCGCTAATGTCCGTTCTAAGACCGTCTG734780.10351103257882895No Hit
GGGGGATCCGTTAGCTATCGTTCGCGAGAAAGTTAGTAGACACACAGGAC724490.10206144423233592No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)