FastQCFastQC Report
Wed 22 Feb 2023
EGAF00007700204

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007700204
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78316211
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATG5345020.682492159892669TruSeq Adapter, Index 2 (97% over 36bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1636310.20893630821848622No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT1383140.1766096676970238TruSeq Adapter, Index 2 (97% over 37bp)
CACCCGTTTGCCTGTCAGTGGTTTCACGTCCTGTTGAACTCTCTCTTCAA1196870.1528253199072667No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)