FastQCFastQC Report
Wed 22 Feb 2023
EGAF00007700247

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007700247
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87379076
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCTC17191621.9674756002226437Illumina Single End PCR Primer 1 (96% over 31bp)
CCGGGCCCGGCGCGCCGGGGGCCGCTACCGGCCTCACACCGTCCACGGGC4247590.48611065651460994No Hit
CGGAAACCTTGTTACGACTTTTACTTCCTCTAGATAGTCAAGTTCGACCG2256890.2582872357221997No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCCTCTATGTGTAGATCT1939290.221939861208878Illumina Single End PCR Primer 1 (96% over 32bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1718130.19662945394387094No Hit
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGAC1514320.173304647899916No Hit
CCCGGGCCCGGCGCGCCGGGGGCCGCTACCGGCCTCACACCGTCCACGGG1458280.16689121317785507No Hit
CCTTCCGCAGGTTCACCTACGGAAACCTTGTTACGACTTTTACTTCCTCT1073080.12280743275426716No Hit
CCCGACTCCGGGAAGACCCGGGCCCGGCGCGCCGGGGGCCGCTACCGGCC964660.11039942789049406No Hit
GTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACC952720.10903296803001213No Hit
GCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGC908520.10397454878099191No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)