FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007700631

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007700631
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99190
Sequences flagged as poor quality0
Sequence length246-251
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA77067.768928319387035No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA61916.241556608529086No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA45234.55993547736667No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG38443.87539066438149No Hit
CCTGTTTGATCCCCGCACTTTCGTGCATCAGCGTCAATCGCGGTTTCGTG34063.4338138925294888No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA25532.5738481701784455No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAA25212.541586853513459No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA20292.045569109789293No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG15281.5404778707531002No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA13961.4073999395100312No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACTGTCCAGAA11281.1372114124407702No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA9600.9678394999495916No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGCA8460.8529085593305776No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG8410.8478677286016735No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTT8180.8246799072487145No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA8030.8095574150620023No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTTACAGCTTAGCA7970.8035084181873172No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTCCAGTA7830.7893940921463857No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA7710.7772960983970159No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTG7520.7581409416271802No Hit
CCTGTTCGATCCCCACGCTTTCGCGCCTCAGCGTCGATCCCGTGCTCGTG7440.7500756124609336No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA6860.6916019760056458No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA6430.6482508317370702No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA6340.6391773364250428No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTCGCGGCTTAGCA6310.6361528379877004No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGCA6180.6230466780925497No Hit
CCTGTTTGCTCCCCACGCTTTCGCATCTCAGCGTCAGATATTGGCCAGAT5620.5665893739288235No Hit
CCCGTTTGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA5400.5444097187216453No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGTA5320.5363443895553988No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTGCCGTCCAGTA5280.5323117249722754No Hit
CCTATTTGCTCCCCACGCTTTCGGGACTGAGCGTCAGTTATGCGCCAGAT5130.5171892327855631No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACAGTCCAGTT5070.5111402359108781No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAACGTCAGTTACAGTCCAGTA4700.4738380885169876No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA4610.4647645932049602No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAG4560.45972376247605606No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAACAGTCCAGCA4560.45972376247605606No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTCAGTCCAGAA4250.42847061195685043No Hit
CCTATTTGCTCCCCACGCTTTCGTCCCTGACCGTCAGTAATAGCCTAGCA3720.3750378062304668No Hit
CCTGTTCGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTGGCGGCCCAGAA3590.36193164633531605No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCCGTGCCGGCGCGGCA3530.3558826494606311No Hit
CCTATTTGCTACCCACGCTTTCGTGACTGAGCGTCAGTATCGGGCTAGTA3520.3548744833148503No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAA3470.3498336525859462No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG3370.3397519911281379No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA3340.33672749269079544No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAGGCCCAGTA3270.32967032967032966No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGTA2950.2974090130053433No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTA2940.2964008468595625No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGTA2700.27220485936082267No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTATCGTCCAGCA2680.270188527069261No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA2640.2661558624861377No Hit
CCTATTTGCTCCCTGTGCTTTCGTGACTGAGCGTCAGTATTGTGCTGGTA2630.2651476963403569No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTGAACGTCAGTTACTGTCCAGCA2630.2651476963403569No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA2550.2570823671741103No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACAGTCCAGTA2150.2167557213428773No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAG2140.21574755519709649No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGCTGTCCAGCA2140.21574755519709649No Hit
CCTGTTCGATACCCGCACCTTCGAGCATCAGCGTCAGTAATGCTGCGGCA2110.212723056759754No Hit
CCTATTTGCTACCCACGCTTTCGTGCTTCAGTGTCAGCAACAGACCAGAC2070.2086903921766307No Hit
CCTATTTGCTCCCCACGCTTTCGTGCTTCAGTGTCAGCAACAGACCAGAC2020.20364956144772656No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTA2010.20264139530194575No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTCCAGAG1980.19961689686460327No Hit
CCTGTTCGATACCCGCGCCTTCGAGCATCAGCGTCAGTGTGCAACTGGCA1970.19860873071882246No Hit
CCTGTTCGCTCCCCCGGCTTTCGCGCCTCAGCGTCAGTGACGGCCCAGAG1940.19558423228147997No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGTT1860.1875189031152334No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA1800.18146990624054843No Hit
CCTGTTTGATCCCCGCACTTTCGTGCCTCAGCGTCAATCGCGGTTTCGTG1660.16735558019961688No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCCGATATAGATTTGTA1650.16634741405383607No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACTGTCCAGCA1580.1592902510333703No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTCCTGTCCAGAA1490.15021675572134288No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCTCAGTA1420.1431595927008771No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACGGTCCAGAA1370.13811876197197298No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAATGGCGGATTCGTG1370.13811876197197298No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGCCGTCCAGTT1310.13206976509728804No Hit
CCTGTTTGATACCCGCACCTTCGAGCCTCAGCGTCAGTCGGCCACCGGGC1300.1310615989515072No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGCGTCCGTAAAGGTCCAGCA1300.1310615989515072No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCATTGTCAGTCCAGTA1300.1310615989515072No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCATGTCCCAGAG1250.12602076822260308No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACCGTCCAGTA1210.12198810363947979No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAA1190.11997177134791814No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTTCAGTCCAGTA1160.11694727291057566No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCCTCAGCGTCAATAGCGGATTCGTG1140.11493094061901402No Hit
CCTGTTTGCTACCCACGCTTTCGCGCCTCAGCGTCAATACCGGTCCAGAC1120.11291460832745237No Hit
CCTGTTTGCTACCCACACTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGTA1100.11089827603589072No Hit
CCTGTTTGCTACCCATGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGTA1100.11089827603589072No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACGGCCCAGCA1080.10888194374432905No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTG1020.10283294686964412No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGAA1010.10182478072386328No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)