FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007700941

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007700941
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79699
Sequences flagged as poor quality0
Sequence length246-251
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG996512.503293642329263No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA54776.872106299953576No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA41895.256025797061444No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA41285.179487822933789No Hit
CCTGTTCGATACCCGCGCTTTCGAGCTTCAGCGTCAGTTGCGCCCCAGTG24003.0113301296126678No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA16382.0552328134606457No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAG12421.5583633420745555No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA9421.181947075872972No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG9171.1505790536895069No Hit
CCTGTTCGATACCCGCACTTTCGAGCCTCAGCGTCAGTTGCGCTCCGGAT7810.9799370130114556No Hit
CCTATTTGCTCCCCACGCTTTCGGGAATGAGCGTCAGTTGCGGCCCAGAC7800.9786822921241171No Hit
CCTGTTCGATACCCGCGCTTTCGAGCTTCAGCGTCAGTTACGCTCCCGCC5670.7114267431209927No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG5540.6951153715855908No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAACAGTCCAGCA5350.6712756747261571No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTGCCGTCCAGTA5300.6650020702894641No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG5170.6486906987540622No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA5000.6273604436693058No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGTA4320.5420394233302802No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGTA4270.5357658188935871No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA3740.46926561186464066No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTCCAGTT3670.4604825656532704No Hit
CCTGTTCGATACCCGCACTTTCGCGCTTCAGCGTCAGTTGCGCTCCAGTG3540.44417119411786843No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGTA3490.4378975896811754No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA3470.43538814790649816No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTCCAGAG3050.38268987063827653No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTCCAGTA3010.37767098708892205No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA2970.3726521035395676No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTG2920.36637849910287457No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAG2830.3550860111168271No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA2810.3525765693421498No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA2420.303642454735944No Hit
ACTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG2300.28858580408788065No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACCGTCCAGTA2280.28607636231320344No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA2270.2848216414258648No Hit
CCTGTTCGATACCCGCACTTTCGAGCCTCAGCGTCAGTTGCGCTCCAGTG2120.2660008281157856No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA1860.23337808504498175No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTATCGTCCAGCA1710.21455727173490258No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAGAGCCCAGCA1690.21204782996022534No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCTAGTG1670.20953838818554812No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTACCAGTCCAGTA1600.20075534197417783No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACAGTTTAGCA1580.19824590019950064No Hit
CCTGTTCGATACCCGCACTCTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG1500.18820813310079174No Hit
CCTGTTTGCTACCCACACTTTCGCGCCTCAGCGTCAGTTGGTGCCCAGTA1490.18695341221345313No Hit
CCTGTTCGATACCCACGCTTTCGAGCCTCAGCGTCAGTCGCGAGTTGGTA1430.17942508688942144No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCTCAGTA1410.17691564511474422No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCTAGTA1410.17691564511474422No Hit
CCTGTTCGCTCCCCCGGCTTTCGCGCCTCAGCGTCAGTGACGGCCCAGAG1370.17189676156538977No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGCTGTCCAGCA1360.17064204067805117No Hit
CCTGTTTGCTACCCACACTTTCGAGACTCAGCGTCAGTTGGTGCCCAGTA1350.16938731979071256No Hit
CCTGTTCGCTCCCCCGGCTTTCGCGCCTCAGCGTCGGTGACGGCCCAGAG1340.16813259890337395No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTT1310.1643684362413581No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCATTGTCAGTCCAGTA1300.1631137153540195No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTCCAGTA1200.15056650648063338No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAACGTCAGTTACCGTCCAGTA1180.14805706470595617No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTGAACGTCAGTTACTGTCCAGCA1180.14805706470595617No Hit
CCTGTTCGATACCCGCACTTGCGAGCTTCAGCGTCAGTTGCGCTCCAGTG1170.14680234381861754No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGCA1120.14052873938192448No Hit
CATGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG1080.13550985583257005No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCTAGTA1050.1317456931705542No Hit
CCTATTTGCTCCCCACGCTTTCGCGAATGAGCGTCAGTTGCGGCCCAGAC1040.1304909722832156No Hit
ACTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA1020.12798153050853836No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTCAGTCCAGAA1010.12672680962119975No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTAGTGCCCAGTA1000.12547208873386118No Hit
CCTGTTTGCTCCCCACGCTTTCGAGACTCAACGTCAGTTACCGTCCAGTA1000.12547208873386118No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA980.12296264695918394No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCCCCAGTG940.11794376340982948No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCGCATCCCAGGG910.11417960074781364No Hit
CCTGTTCGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTGGCGGCCCAGAA900.11292487986047503No Hit
CCTGTTTGCTACCCACACTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGTA890.11167015897313644No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTTTAGTA880.11041543808579782No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAGGCCCAGTA830.10414183364910476No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCTAGTA830.10414183364910476No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)