FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007701355

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007701355
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43609
Sequences flagged as poor quality0
Sequence length247-251
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA41929.612694627255841No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA36888.45696989153615No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA26015.964365153981976No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA22555.170950950491871No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA12872.951225664427068No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGTA12542.8755532114930404No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG10432.391708133642138No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA6261.4354835011121556No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA5861.3437593157375771No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACCGTCCAGTA5451.249742025728634No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAATGTCAGTTACAGGTTAGTA5411.2405696071911763No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG5351.2268109793849893No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTA4060.9310004815519732No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA3850.8828452842303195No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAATCCAGTA3730.8553280286179459No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA3640.8346900869086656No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAACAGTCCAGCA3570.8186383544681144No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGTA3240.742965901534087No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGTGTCAGTTGCAGCTTAGCA2970.6810520764062464No Hit
CCTATTTGCTCCCCACGCTTTCGGGACTGAGCGTCAGTTATGCGCCAGAT2940.6741727625031531No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTGCCGTCCAGTA2770.6351899837189571No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG2310.5297071705381916No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGTA2260.5182416473663692No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTG2150.49301749638836023No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG2140.49072439175399574No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA2060.47237955467908005No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGCA1820.4173450434543328No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCTCAGTA1730.39670710174505264No Hit
CCTGTTTGCTCTCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA1710.3921208924763237No Hit
CCTGTTTGCTCTCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA1660.38065536930450133No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGCGTCAGTTACAGTCCAGTA1580.36231053222958565No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG1470.33708638125157653No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCTGTTTCACCCTAGCA1460.3347932766172121No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG1400.3210346488110252No Hit
CCTGTTTGCTCCCCACGCTTGCGAGCCTCAGCGTCAGTTATCGTCCAGTA1310.30039670710174504No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAGGCCCAGTA1290.2958104978330161No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCGACGTCAGTTACCGTCCAGTA1240.2843449746611938No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCTAGTG1210.2774656607581004No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTTGTCCAGGG1160.26600013758627805No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA1130.2591208236831846No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA1050.24077598660826893No Hit
CCTGTTTGATATCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA1020.23389667270517553No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA1010.23160356807081106No Hit
CCTGTTTGCTCCCCACGCTTGCGAGCCTCAACGTCAGTTACCGTCCAGTA990.22701735880208215No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAGTCGTCCAGTA990.22701735880208215No Hit
CCTGTTTGCTATCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA960.22013804489898875No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTGAACGTCAGTTACTGTCCAGCA950.21784494026462425No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG920.2109656263615309No Hit
CCTGTTTGATACCCACACTTGCGAGCCTCAATGTCAGTTGCAGCTTAGCA910.2086725217271664No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTAACAGTCTAGTG910.2086725217271664No Hit
CCTGTTTGCTACCCACACTTGCGAGCCTCAGCGTCAGTTGGTGCCCAGTA890.2040863124584375No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCTCAGTA870.19950010318970854No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTGACAGTCTAGTG850.1949138939209796No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGCGTCAGTTACAGTCCAGCA830.19032768465225067No Hit
CCTGTTTGATCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA770.1765690568460639No Hit
CCTGTTTGCTATCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA730.16739663830860604No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGCTGTCCAGCA710.16281042903987708No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCTTGGCCCAGGA700.1605173244055126No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTCAGCGTCAGTTACCGTCCAGGT640.14675869659932583No Hit
CCTGTTTGATATCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGTA610.13987938269623243No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTACAGGTTAGTA550.12612075489004565No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA540.12382765025568117No Hit
CCTGTTTGATCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA530.12153454562131669No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTT530.12153454562131669No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGCGTCAGTTACAATCCAGTA520.11924144098695223No Hit
CCTGTTTGCTCCCCACGCTCTCGAGCCTCAACGTCAGTTACCGTCCAGTA510.11694833635258776No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCTAGTA500.11465523171822331No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA490.11236212708385883No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACTGTCCAGTA480.11006902244949437No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTCCAGTT460.10548281318076545No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG450.10318970854640097No Hit
CCTGTTTGCTCCCCACGCTTTCGTGACTCAGCGTCAGTAACAGTCCAGTA450.10318970854640097No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)