FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007701509

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007701509
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47258
Sequences flagged as poor quality0
Sequence length244-251
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA684914.492784290490498No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG621313.146980405434No Hit
CCTGTTTGATACCCACACTTTCGTGCCTCAGTGTCAGTTATAGTTTAGTA31216.604172838461213No Hit
CCCGTTTGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA31116.583012400016928No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGAA24295.1398704981167205No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA15633.3073765288416777No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGCA15393.256591476575395No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA14833.138093021287401No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTATCGTCCAGTA12492.642938761691142No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA10502.2218460366498793No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG8351.7668966100977612No Hit
CCTGTTCGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTCGTGGCCCAGAA8191.733039908586906No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTA7551.5976131025434845No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTGATCGTCCAGTA6611.3987049811672096No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACTGTCCAGCA6041.2780904820347878No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA3830.8104447924160988No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTTGTCCAGGG3180.6729019425282492No Hit
CCTATTTGCTCCCCACGCTTTCGGGACTGAGCGTCAGTTGCAGGCCAGAT2940.6221168902619663No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA2920.6178848025731093No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACCGTCCAGTA1700.35972745355283764No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTA1530.32375470819755386No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGCG1510.31952262050869695No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGCA1440.30471031359769774No Hit
CCTATTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGACCAGGC1420.3004782259088408No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTACAGCCCAGCA1120.23699691057598712No Hit
CCTGTTTGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG1100.23276482288713024No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTGGTCGTCCAGTA1010.2137204282872741No Hit
GCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA930.19679207753184647No Hit
CCTGTTTGATCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA840.17774768293199036No Hit
CCTGTTTGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA780.1650514198654196No Hit
GCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG730.15447120064327732No Hit
CCTATTTGCTCCCCACGCTTTCGGGAATGAGCGTCAGTTACAGGCCAGAC680.14389098142113504No Hit
GCTGTTTGATACCCACACTTTCGTGCCTCAGTGTCAGTTATAGTTTAGTA660.13965889373227813No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCTCAGAG630.13331076219899277No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTATCGGCCCAGAG620.1311947183545643No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTGCCGTCCAGTA620.1311947183545643No Hit
CCTGTTTGATACCCACACTTTCGCGCCTCAGTGTCAGTTATAGTTTAGTA610.12907867451013585No Hit
CCTGTTCGATCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG590.12484658682127894No Hit
CCTGTTTGCTACCCATGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGTA590.12484658682127894No Hit
CCTGTTCGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTTGCGGCCCAGCA550.11638241144356512No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGTGTCAGTTATAGTTTAGTA540.11426636759913665No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)