FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007701995

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007701995
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71547
Sequences flagged as poor quality0
Sequence length246-251
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA63248.838945029141684No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA25463.558500006988413No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA20152.8163305239912226No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA19802.767411631514948No Hit
CCTATTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTAACGGCCCAGCA17362.426377066823207No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA14922.085342502131466No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA13851.9357904594182844No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTATCGTCCAGCA13231.8491341356031699No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG11961.6716284400464032No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGCA11011.5388485890393728No Hit
CCTGTTCGATACCCACGCTTTCGAGCCTCAGCGTCAGTCGCGAGTTGGTA10471.4633737263616922No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTCCAGAA10181.4228409297384936No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA8251.153088179797895No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA8031.1223391616699512No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA7741.0818063650467524No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGTA7100.9923546759472793No Hit
CCCGTTTGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA7010.9797755321676659No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA6780.9476288313975428No Hit
CCTGTTTGCTCCCCACGCTTTCGAGACTCAACGTCAGTTACCGTCCAGTA6540.9140844479852405No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTTCTGTCCAGAA6210.8679609207933247No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACAGTCCAGTT5890.8232350762435882No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTG5810.8120536151061539No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG5730.8008721539687199No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAG5670.7924860581156443No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGTA5490.7673277705564174No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAA5320.74356716563937No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACTGTCCAGTA4600.6429340154024626No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA4410.6163780452010567No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACAGTCCAGTA4290.5996058534949054No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTTCAGTCCAGAA4250.5940151229261884No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG4170.5828336617887542No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAGCGTCAGTGCTGCCCCGGCA3950.5520846436608104No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTGCCGTCCAGTA3680.5143472123219701No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCATTGTCAGTCCAGTA3670.5129495296797909No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTA3380.47241673305659215No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATACCGGTCCAGGT3300.46123527191915803No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTCAGTCCAGAA3250.4542468587082617No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA3100.4332816190755727No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACGGTCCAGAA2890.40393028358980804No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACTGTCCAGAA2760.38576040924147764No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTCCAGTT2750.3843627265992984No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCTGTCGCACCCTAGCA2620.36619285225096787No Hit
CCTGTTTGCTACCCACACTTTCGAGACTCAGCGTCAGTTGGTGCCCAGTA2540.35501139111353375No Hit
CCTATTTGCTCCCCACGCTTTCGTCCCTGACCGTCAGTAATAGCCTAGCA2520.3522160258291752No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA2450.34243224733392036No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA2330.3256600556277692No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTATTGTCCAGAA2180.30469481599508014No Hit
CCTGTTCGCTCCCCCGGCTTTCGCGCCTCAGCGTCAGTGACGGCCCAGAG2180.30469481599508014No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTACCGTCCAGTA2080.2907179895732875No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA2030.28372957636239116No Hit
CCTGTTTGCTCCCCACGCTTTCGCGACTCAGCGTCAGTTGTCGTCCAGCA2030.28372957636239116No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTG1980.27674116315149483No Hit
CCTGTTTGCTCCCCACGCTTTCGCGACTCACCGTCAGTTGTCGTCCAGCA1960.2739457978671363No Hit
CCTGTTTGCTACCCATGCTTTCGAGACTCAGCGTCAGTTGGTGCCCAGTA1860.2599689714453436No Hit
CCTATTTGCTCCCCACGCTTTCGTGACTCAGCGTCAGTAACGGCCCAGCA1830.2557759235188058No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAACGTCAGTTACCGTCCAGTA1740.24319677973919243No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA1710.2390037318126546No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAG1710.2390037318126546No Hit
CCTATTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTAACGGCTCAGCA1630.22782227067522048No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG1600.22362922274868266No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACTGTCCAGCA1600.22362922274868266No Hit
CCTGTTTGCTCCCCACACTTTCGCGACTCACCGTCAGTTATCGTCCAGCA1600.22362922274868266No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACACTCCAGTG1440.20126630047381439No Hit
CCCATTTGCTACCCTGGCTTTCGTTCCTCAGCGTCAATTACGGCCCAGTA1420.19847093518945588No Hit
CCTGTTTGCTCCCCACGCTTTCGAGACTCAGCGTCAGTTATCGTCCAGTA1400.19567556990509735No Hit
CCTGTTCGCTCCCCCAGCTTTCGCTCCTCAGCGTCAGTCGAGGCCCAGAA1360.1900848393363803No Hit
CCTATTTGCTACCCACGCTTTCGTACCTCAGCGTCAGTTACAGCCCAGCA1320.1844941087676632No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTCATCGTCCAGTA1290.1803010608411254No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTA1260.1761080129145876No Hit
CCTGTTTGCTCCCCACGCTTTCGTGACTCAGCGTCAGTTACAGTCCAGAA1160.16213118649279495No Hit
CCTGTTTGATCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA1140.15933582120843642No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG1090.15234740799754007No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACCGTCCAGTA1040.14535899478664374No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTCAGCGTCAGTTACCGTCCAGGT1030.14396131214446448No Hit
CCTGTTTGCTCCCCACACTTTCGAGACTCAGCGTCAGTTAAAGCCCAGCA1020.1425636295022852No Hit
CCTGTTCGATACCCACGCTTTCGAGACTCAGCGTCAGTCGCGAGTTGGTA1020.1425636295022852No Hit
CCTGTTTGATACCCACACTTTCGAGACTCAATGTCAGTTGCAGCTTAGCA1000.13976826421792668No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTACCGTCCAGTA990.13837058157574741No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTACCAGTCCAGTA980.13697289893356815No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAGTCGTCCAGTA980.13697289893356815No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTAATAGCTTGGAA980.13697289893356815No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGCGGCCCAGTA950.13277985100703035No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTGCTGTCCAGCA860.12020070722741695No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCTCAGTA850.11880302458523767No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTGAACGTCAGTTACTGTCCAGCA840.1174053419430584No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCATTTACCGTCCAGTA820.11460997665869987No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCATCAGCGTCAGTAATAGCTTGGAA820.11460997665869987No Hit
CCTGTTCGCTCCCCACGCTTTCGAGACTCAGCGTCAGTTACAGTCCAGAA800.11181461137434133No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTTGCGTCCCAGGG800.11181461137434133No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCTCAGTA780.10901924608998281No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTA780.10901924608998281No Hit
CCTGTTTGCTCCCCACACTTTCGTGACTCAACGTCAGTTTCTGTCCAGAA760.10622388080562427No Hit
CCTGTTTGCTCCCCACGCTTTCGAGACTCAACGTCAGTCATCGTCCAGCA750.10482619816344502No Hit
CCTATTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTAACGGCCCAGCA740.10342851552126574No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTT730.10203083287908647No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)