FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007702035

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007702035
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95665
Sequences flagged as poor quality0
Sequence length241-251
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA70147.331835049391104No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA49525.176396801338003No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA34663.623059635185282No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA22892.392724611927037No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA21672.2651962577745257No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTG18261.9087440547744736No Hit
CCTGTTTGATCCCCATGCTTTCGTGCCTCAGCGTCAGTATATGGCCAGAT14601.5261589923169392No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA13671.4289447551351069No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACAGTCCAGTT13271.387132180003136No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA11931.2470600533110334No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA11491.201066220665865No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA10841.1331207860764125No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA9941.0390424920294778No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGCA9661.009773689437098No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACGGCTTAGTG9550.9982752312758062No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTATCGTCCAGCA8840.9240579104165578No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA8730.9125594522552658No Hit
CCTGTTTGCTCCCCACGCTTTCGTGTCTCAGCGTCAGTATTGGCCTAGTG8460.8843359640411854No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGTA7890.8247530444781268No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGTA6810.7118590916218053No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTCCAGTA6760.7066325197303089No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCATGTCCCAGAG6580.687816860920922No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA6550.6846809177860241No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGTGTCAGTCATGGCTTGGCA6410.6700465164898344No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGCA6270.6554121151936445No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTTCTGTCCAGAA6200.6480949145455496No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTGCCGTCCAGTA5980.6250979982229655No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA5460.5707416505514034No Hit
CCTGTTCGATACCCGCGCTTTCGAGCTTCAGCGTCAGTTACGCTCCCGCC4760.49756964407045423No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA4350.454711754560184No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTA4160.4348507813724978No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACTGTCCAGAA3680.38467569121413264No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAGTCGTCCAGTA3550.37108660429624213No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTCCAGTA3530.36899597553964353No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG3510.366905346783045No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCTGTCGCACCCTAGCA3500.36586003240474574No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCATTGTCAGTCCAGTA3500.36586003240474574No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGTGTCAGTCATGGCTTTGCA3380.35331625986515447No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA3350.3501803167302566No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAACAGTCCAGCA3260.34077248732556314No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGCTGTCCAGCA3240.33868185856896466No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTTCAGTCCAGAA3180.33240997229916897No Hit
CCTGTTTGATACCCGCACTTTCGAGCATCAACGTCAGTTACGGTCCAGCA3040.31777557100297915No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGTA2860.2989599121935922No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTCAGTCCAGAA2810.29373334030209586No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA2760.28850676841059947No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACCGTCCAGTA2750.2874614540323002No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG2750.2874614540323002No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAATGTCAGTTACAGGTTAGTA2700.28223488214080383No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAATGTCAGTTACAGTCCAGTA2680.2801442533842053No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGTA2400.2508754507918256No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG2310.24146762138713218No Hit
CCTGTTTGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTTAATGTCCAGCA2290.23937699263053364No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCATTTACAGTCCAGTA2150.22474259133434382No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAACGTCAGTTACAGTCCAGTA2110.2205613338211467No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTACCGTCCAGTA2100.21951601944284743No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACACTCCAGTG2040.21324413317305183No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCTAGTA2000.2090628756598547No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTGCTGTCCAGCA1810.1892019024721685No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAACGTCAGTTACCGTCCAGTA1730.18083938744577432No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGTGTCAGTCATGGCTTGGGA1670.1745675011759787No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATACCGGTCCAGGT1670.1745675011759787No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTCATCGTCCAGTA1580.1651596717712852No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTTTAGTA1490.15575184236659176No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG1460.1526158992316939No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGTT1440.1505252704750954No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCTCAGTA1380.14425338420529973No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTTAGTGTCAGTTTCAGTCCAGTA1360.14216275544870122No Hit
CCTGTTTGCTCCCCACACTTTCGTGTCTCAGCGTCAGTAATCGTCCAGTA1310.13693618355720483No Hit
CCTGTTCGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTGGCGGCCCAGAA1250.1306642972874092No Hit
CCTATTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTATGGCCCAGTA1240.1296189829091099No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCAGTTTCGGCCCAGAG1220.12752835415251138No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTTATGTCCCAGGA1200.1254377253959128No Hit
CCTGTTTGCTACCCACACTTTCGCGCCTCAGCGTCAGTTGGTGCCCAGTA1190.12439241101761356No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTGAACGTCAGTTACTGTCCAGCA1190.12439241101761356No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCTAGTA1140.11916583912611718No Hit
CCTGTTTGCTCCCCACGCTTTCGTGTCTCAGCGTCAGTTGTGGTCCAGTA1130.11812052474781791No Hit
CCTGTTTGCTCCCCACGCTTTCGTGTCTCAGCGTCAGTGGTCGTCCAGTA1120.11707521036951862No Hit
CCTATTTGCTCCCCACGCTTTCGGGACTGAGCGTCAGTTATGCGCCAGAT1120.11707521036951862No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAGTGTCCAGCA1080.11289395285632153No Hit
CCTGTTTGCTCCCCACGCTTTCGTACCTCAGCGTCAGTTACAGTCCAGAA1070.11184863847802226No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTCCAGTT1070.11184863847802226No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCTAGTA1060.110803324099723No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGTGTCAGTCATGGCTTTGGA1030.10766738096482517No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAG990.10348612345162807No Hit
CCTGTTCGATACCCACGCTTTCGTGCTTCAGCGTCAGTTATAGTATGGTA970.10139549469502954No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)