FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007702312

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007702312
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51716
Sequences flagged as poor quality0
Sequence length248-251
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGAAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA1594030.822182690076573No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA1488728.7860623404749No Hit
TACGTAGGTGGCAAACGTTGTCCGGATTTATTGGGCGTAAAGGGAGCGCA684513.235749091190346No Hit
TACGTAGGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA20493.9620233583417126No Hit
TACGTAGGGTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA9851.9046329955913064No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA7391.4289581560832239No Hit
TACGTAGGGTGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGGGCTCGTA5130.9919560677546602No Hit
TACGTAGGGTGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA4890.9455487663392375No Hit
TACGTATGTCCCGAGCGTTATCCGGATTTATTGGGCGTAAAGCGAGCGCA3050.5897594554876634No Hit
TACGCAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA2400.4640730141542269No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA2360.45633846391832317No Hit
TACGTAGGGCGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA2310.44667027612344345No Hit
TACGTAGGTGGCAACCGTTGTCCGGATTTATTGGGCGTAAAGGGAGCGCA2120.40993116250290046No Hit
TACGGAGGGTGCAAGCGTTACTCGGAATTACTGGGCGTAAAGCGCACGCA1620.31324928455410317No Hit
TACGAAGGGTGCAAGCGTTACTCGGAATTACTGGGCGTAAAGCGCGCGTA1200.23203650707711346No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA1070.20689921881042617No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAGGCGCACGCA950.1836955681027148No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA940.18176193054373888No Hit
TACGAAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAGGCGCGCGTA930.17982829298476294No Hit
TACGAAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA730.14115554180524403No Hit
TACGTAGGGTGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTTGTA660.12762007889241242No Hit
TACGGAGGGTGCAAGCGTTATTCGGAATTACTGGGCGTAAAGCGCACGCA660.12762007889241242No Hit
TACGGAGGGTGCAAGCGTTAATCGGAGTTACTGGGCGTAAAGCGCACGCA630.12181916621548457No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGCA620.11988552865650863No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA620.11988552865650863No Hit
TATGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA590.11408461597958079No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGGGAACGCA580.11215097842060483No Hit
TACGAAGGGTGCAAGCGTTATTCGGAATTACTGGGCGTAAAGCGCGCGTA560.10828370330265295No Hit
TACGAAGGGTGCAAGCGTTGATCGGAATTACTGGGCGTAAAGCGCGCGTA540.10441642818470105No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)