FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007702315

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007702315
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79301
Sequences flagged as poor quality0
Sequence length246-251
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTG52846.6632198837341265No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA45465.732588491948399No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA40095.055421747518947No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA16592.092029104298811No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG16142.0352832877265103No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGCA15641.9722323804239543No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAGCGTCAGTTGCACCCCGGAT14891.8776560194701202No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA13781.7376830052584458No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA12411.564923519249442No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA11381.4350386502061765No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTCCAGTA10601.336679234814189No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACAGTCCAGTT10321.3013707267247576No Hit
CCTGTTCGATACCCGCGCTTTCGAGCTTCAGCGTCAGTTGCGCCCCAGTG7420.9356754643699323No Hit
CCTATTTGCTCCCCACGCTTTCGTGCTTCAGTGTCAGAATCCAGACCAGA7350.9268483373475744No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG6890.8688415026292229No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA6350.8007465227424623No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA6020.7591329239227752No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA5950.7503057969004174No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA5790.7301295065635994No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAGGCCCAGTA5390.6796887807215546No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGTA4710.5939395467900783No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAACAGTCCAGCA4670.5888954742058738No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGCA3980.5018852221283464No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG3980.5018852221283464No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAGCGTCAGTTATGCCCCGGCA3960.49936318583624417No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTG3490.4400953329718415No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTATCGTCCAGCA3320.4186580244889724No Hit
CCTGTTTGCTCCCCACGCTTTCGAGACTCAACGTCAGTTACCGTCCAGTA3250.40983089746661455No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTCCAGAG3170.39974275229820555No Hit
CCTGTTTGCTACCCACACTTTCGAGACTCAGCGTCAGTTGGTGCCCAGTA3160.39848173415215443No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA3020.3808274801074387No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGTA2850.3593901716245697No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGTT2740.34551897201800735No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA2680.3379528631417006No Hit
CCTGTTTGCTCCCCACGCTTTCGTGTCTCAGCGTCAGTAATGGTCCAGTA2670.33669184499564947No Hit
CCTGTTCGATACCCACGCTTTCGAGCCTCAGCGTCAGTCGCGAGTTGGTA2620.3303867542653939No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGTA2420.30516639134437146No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTGCCGTCCAGTA2360.29760028246806475No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCATGTCCCAGAG2290.28877315544570686No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACGGTCCAGAA2280.28751213729965575No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGTA2220.27994602842334904No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA2140.26985788325494003No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCATTGTCAGTCCAGTA2100.26481381067073556No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTCAGTCCAGAA2030.2559866836483777No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCTGTTTCACCCTAGCA1990.2509426110641732No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTCCAGTT1960.24715955662601985No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGCTGTCCAGCA1830.23076632072735526No Hit
CCTGTTTGATACCCACACTTTCGAGACTCAATGTCAGTTGCAGCTTAGCA1630.20554595780633284No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTGCTGTCCAGCA1600.20176290336817948No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTCATCGTCCAGTA1600.20176290336817948No Hit
CCTATTTGCTCCCCACGCTTTCGGGACTGAGCGTCAGTTATGCGCCAGAT1600.20176290336817948No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACCGTCCAGTA1500.18915272190766824No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA1480.186630685615566No Hit
CCTGTTCGCTCCCCACGCTTTCGCTACTCAGCGTCAGTGACGGCCCAGAG1460.18410864932346377No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG1450.18284763117741265No Hit
CCTTTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTG1450.18284763117741265No Hit
TCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCCGTG1430.18032559488531041No Hit
CCTTTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA1340.1689764315708503No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACTGTCCAGAA1310.16519337713269694No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAGAGCCCAGCA1280.16141032269454358No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAG1260.15888828640244132No Hit
CCTTTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA1240.15636625011033908No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCTCAGTA1220.15384421381823685No Hit
TCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA1180.14880014123403237No Hit
CCTGTTCGATACCCGCACTTTCGCGCTTCAGCGTCAGTTGCGCTCCCGTG1160.1462781049419301No Hit
CCTGTTTGCTCCCCACGCTTTCGAGACTCAGCGTCAGTAAAAGCCCAGCA1140.14375606864982787No Hit
CCTGTTTGATACCCGCACTTTCGAGACTCAGCGTCAGTTGCACCCCGGAT1090.13745097791957225No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTGAACGTCAGTTACTGTCCAGCA1090.13745097791957225No Hit
CCTGTTCGCTACCCACGCTCTCGCGCATCAGCGTCAGATCGAGCCTGGGA1010.12736283275116328No Hit
TCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA1000.12610181460511216No Hit
CCTATTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTGATGGCCCAGCA950.11979672387485654No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTACCGTCCAGTA950.11979672387485654No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG950.11979672387485654No Hit
CCTGTTTGCTCCCCACACTTTCGCGACTCAGCGTCAGTTACAGTCCAGTT910.11475265129065207No Hit
CCTGTTTGCTCCCCACGCTTTCGCGACTCAGCGTCAGTTGTCGTCCAGAA900.11349163314460095No Hit
CCTGTTTGCTACCCATGCTTTCGAGACTCAGCGTCAGTTGGTGCCCAGTA870.1097085787064476No Hit
CCTGTTCGATACCCGCACTTTCGAGCCTCAGCGTCAGTTGCGCTCCCGTG860.10844756056039646No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAACGTCAGTTACCGTCCAGTA850.10718654241434535No Hit
CCTGTTTGATCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA850.10718654241434535No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAACAGCCCAGCA830.1046645061222431No Hit
CCTGTTTGCTCCCCACGCTTTCGAGACTCAGCGTCAGTTATCGTCCAGTA830.1046645061222431No Hit
CCTTTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG820.10340348797619199No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAGGCCCAGCA810.10214246983014086No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)