FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007702363

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007702363
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133060
Sequences flagged as poor quality0
Sequence length245-251
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA126619.515256275364498No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG107348.067037426724784No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA74235.578686306929205No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA45833.4443108372162934No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA42923.2256125056365548No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA41303.103862918983917No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA32802.4650533593867427No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCTAGTG19641.4760258529986472No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG18021.3542762663460095No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG15891.1941981061175408No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGTA13000.9770028558545018No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA12920.9709905305877047No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG12450.9356681196452727No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGTGTCAGTTGCAGCTTAGCA11360.8537501878851645No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTCCAGAG11360.8537501878851645No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCTCAGAG10840.8146700736509846No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCTCAGTA8880.6673681046144597No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTGAACGTCAGTTACTGTCCAGCA8680.6523372914474673No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA8350.62753644972193No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA8230.6185179618217346No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGCA7950.5974748233879452No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACCGTCCAGTA7000.5260784608447316No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAG6650.49977453780249514No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG6590.49526529385239737No Hit
CCTGTTTGCTATCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA6090.45768826093491655No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAG5990.4501728543514204No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTATCGGCCCAGAG5450.4095896588005411No Hit
CCTGTTCGCTCTCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG5150.38704343905005256No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAATGTCAGTTACAGGTTAGTA4820.36224259732451525No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTGAACGTCAGTTACTGTCCAGTA4730.3554787313993687No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG4610.3464602434991733No Hit
CCTGTTTGCTACCCACACTTGCGAGCCTCAGCGTCAGTTGGTGCCCAGTA4520.33969637757402676No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA4380.3291748083571321No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGAA4310.32391402374868483No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCACCGTCCAGTA4260.3201563204569367No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAATCCAGTA3950.2968585600480986No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA3940.296107019389749No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGCG3720.27957312490605746No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGTA3640.2735607996392605No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAACAGTCCAGCA3640.2735607996392605No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAA3610.2713061776642116No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTA3610.2713061776642116No Hit
CCTGTTTGATATCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA3570.2683000150308132No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG3450.25928152713061775No Hit
CCTGTTCGCTCCCCACGCTTGCGCTCCTCAGCGTCAGTAACGGCCCAGAG3400.2555238238388697No Hit
CCTATTTGCTCCCCACGCTTTCGGGACTGAGCGTCAGTTGCAGGCCAGAT3370.2532692018638208No Hit
CCTGTTTGATACCCACACTTGCGAGCCTCAATGTCAGTTGCAGCTTAGCA3320.24951149857207275No Hit
CCTATTTGCTCCCCACGCTTTCGGGACTGAGCGTCAGTTGCAGCCCAGAT2880.21644370960468962No Hit
CCTATTTGCTCCCCACGCTTTCGGGACTGAGCGTCAGTTATGCGCCAGAT2690.2021644370960469No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTGCCGTCCAGTA2540.19089132722080265No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGTA2430.18262437997895686No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTG2390.1796182173455584No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCTCAGCA2370.17811513602885917No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA2260.16984818878701338No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA2230.16759356681196452No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAAGCCAGAG2190.16458740417856604No Hit
CCTGTTTGCTCTCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA2180.16383586352021645No Hit
CCTGTTTGCTCTCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA2100.15782353825341952No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGTGTCAGTTGCAGTCTAGTG2070.15556891627837066No Hit
CCCGTTCGCTCTCCTGGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA1890.14204118442807756No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCTAGTA1770.13302269652788215No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCTTGGCA1770.13302269652788215No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCGACGTCAGTTACCGTCCAGTA1770.13302269652788215No Hit
CCTGTTTGCTCCCCACGCTTGCGAGCCTCAGCGTCAGTTATCGTCCAGTA1760.13227115586953253No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAACGTCAGTTACAGTCCAGAA1690.12701037126108525No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATCAGTGTCAGTGACAGTCCAGTA1630.12250112731098753No Hit
CCTGTTTGCTACCCACACTCTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA1620.12174958665263791No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTATCGGCTCAGAG1600.12024650533593868No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGCGTCAGTTACAGTCCAGTA1550.1164888020441906No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCTGTTTCACCCTAGCA1520.11423418006914172No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGCGCCCAGTA1450.10897339546069443No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGTCCCAGAG1430.1074703141439952No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCTCAGAG1400.10521569216894633No Hit
CCTGTTTGCTCCCCACGCTTGCGAGCCTCAACGTCAGTTACCGTCCAGTA1390.10446415151059672No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG1360.10220952953554788No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA1340.10070644821884864No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)