FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007702933

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007702933
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142255
Sequences flagged as poor quality0
Sequence length244-251
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG3921327.565287687603245No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA68244.797019436926646No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA53023.727109767670732No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA38632.7155460265017046No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA27821.9556430353941865No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA27781.9528311834381922No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA22591.5879933921479035No Hit
CCTGTTCGATACCCGCACTTTCGCGCTTCAGCGTCAGTTGCGCTCCAGTG19041.3384415310533901No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG18841.3243822712734175No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTA18511.3011844926364626No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA17461.2273733787916066No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA16781.1795718955396999No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA15261.0727215212119083No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG10300.7240518786685881No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG9460.6650029875927033No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA9100.6396963199887526No Hit
CCTGTTCGATACCCGCACTTTCGAGCCTCAGCGTCAGTTGCGCTCCAGTG8950.6291518751537731No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA8400.5904889107588486No Hit
CCTGTTTGCTACCCACGCTTTCGAACCTCAGCGTCAGTTACAGACCAGAG7720.5426874275069418No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGATGCCCAGTA7690.5405785385399459No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCTAGTG7640.5370637235949527No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAATGTCAGTTACAGCTTGGTG7210.5068363150680117No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTG7190.5054303890900144No Hit
CCTGTTCGATACCCGCACTCTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG6970.4899652033320445No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAGGCCCAGTA6660.468173350673087No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGCA6500.456925942849109No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAACAGTCCAGCA6400.44989631295912275No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTTATGTCCCAGGA6330.4449755720361323No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGTA6050.42529260834417065No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG5970.41966890443218163No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGTA5910.4154511264981899No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTCCAGTA4720.331798530807353No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTCCAGAG4500.31633334504938315No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA4230.29735334434642013No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTTTAGTA3910.274858528698464No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTGCCGTCCAGTA3860.2713437137534709No Hit
CCTGTTCGATACCCGCACTTGCGAGCTTCAGCGTCAGTTGCGCTCCAGTG3800.26712593581947913No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG3680.2586903799514956No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG3530.24814593511651611No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGCA3490.2453340831605216No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA3330.2340866753365435No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTTCGCTCCAGTG3270.22986889740255176No Hit
CCTGTTCGATACCCGCACTTTCGATCTTCAGCGTCAGTTGCGCTCCAGTG3260.22916593441355315No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAATGTCAGTTACAGGTTAGTA3250.2284629714245545No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACCGTCCAGTA3210.22565111946855998No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGATGCGCTCCAGTG3130.22002741555657096No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTGAACGTCAGTTACTGTCCAGCA2950.20737408175459565No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCATTTGCGCTCCAGTG2720.19120593300762714No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAG2570.1806614881726477No Hit
CCTGTTTGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG2530.17784963621665317No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTT2280.16027556149168745No Hit
CCTGTTTGCTACCCACACTTTCGAGACTCAGCGTCAGTTGGTGCCCAGTA2060.1448103757337176No Hit
CCTATTTGCTCCCCACGCTTTCGGGACTGAGCGTCAGTTATGCGCCAGAT1870.13145407894274366No Hit
CCTGTTTGCTACCCACACTTTCGCGCCTCAGCGTCAGTTGGTGCCCAGTA1860.13075111595374506No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTATCGTCCAGCA1840.1293451899757478No Hit
CCTGTTCGATACCCTCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG1830.12864222698674913No Hit
CCTGTTTGCTCCCCACGCTTTCGAGACTCAGCGTCAGTTATCGTCCAGTA1720.12090963410776423No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTCCAGTA1680.1180977821517697No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGAG1640.1152859301957752No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTACGCTCCAGTG1630.11458296720677658No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCATTGTCAGTCCAGTA1620.11388000421777794No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCTCAGTA1600.11247407823978069No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTTAGTTGCGCTCCAGTG1530.10755333731679026No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCTAGTA1520.10685037432779164No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTATTCGTCCAGTA1480.10403852237179713No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA1430.10052370742680398No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)