FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007703069

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007703069
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51499
Sequences flagged as poor quality0
Sequence length246-251
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTACCCACGCTTTCGCATATGAGCGTCAGTATTTGCCCAGGA593211.518670265442047No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG545610.59438047340725No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA21564.186489058039962No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTCCAGTT16943.2893842598885414No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA13842.687430823899493No Hit
CCCGTTTGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA13612.6427697625196607No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA13152.5534476397599954No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAA10472.033049185421076No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA10111.9631449154352512No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA9551.8544049399017457No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA9381.821394590186217No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTCCAGAA9111.7689663876968484No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG6791.3184722033437541No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTCCAGTA6151.1941979455911764No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA5981.1611875958756481No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTA5511.0699236878385987No Hit
CCTATTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTAACGGCCCAGCA5291.02720441173615No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTCATCGTCCAGTA4380.8505019514942038No Hit
CCTATTTGCTCCCCACGCTTTCGGGAATGAGCGTCAGTTGCGGCCCAGAC4280.8310840987203635No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA4240.8233169576108275No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG4130.8019573195596031No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA4030.7825394667857628No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA3850.7475873317928503No Hit
CCTGTTTGCTACCCACGCTTTCGCCTATGAGCGTCAGTATTTGCCCAGGA3530.6854502029165614No Hit
CCTGTTTGCTACCCACGCTTTCGCATATGAGCGTCAGTATTTGCCCAGTA3430.6660323501427212No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGTA3180.6174877182081205No Hit
CCTGTTCGATACCCACGCTTTCGAGCCTCAGCGTCAGTCGCGAGTTGGTA3150.6116623623759685No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTATCGTCCAGCA2830.5495252334996796No Hit
CCTGTTTGCTCCCCACGCTTTCGTGTCTCAGCGTCAGTAATGGTCCAGTA2710.5262238101710712No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAACAGTCCAGCA2700.5242820248936872No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA2160.41942561991494987No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTG2160.41942561991494987No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTT2130.4136002640827977No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA2090.4058331229732616No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTGCTGTCCAGCA2030.3941824113089574No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAG2020.3922406260315734No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA1900.3689392027029651No Hit
CCCTTTCGCTCCCCTGGCCTTCGTGCCTCAGCGTCAGTTAATGTCCAGGA1890.36699741742558106No Hit
CCTATTTGCTCCCCACGCTTTCGTGCTTCAGTGTCAGCAACAGACCAGAC1890.36699741742558106No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAG1830.3553467057612769No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAGCGTCAGTTGCACCCCGGAT1830.3553467057612769No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGCA1770.34369599409697277No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTGTCAGTCCAGAA1760.34175420881958873No Hit
CCTGTTTGCTACCCACGCTTTCGCATCTGAGCGTCAGTATTTGCCCAGGA1750.3398124235422047No Hit
CCTGTTTGATTCCCGCACTTTCGAGCCTCAGCGTCAGTAACACCCCAGAA1710.3320452824326686No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATACCGGTCCAGGT1630.31651100021359635No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTTTTGCCCCAGGG1590.30874385910406027No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGTA1530.2970931474397561No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGCTGTCCAGCA1490.28932600633022004No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTGCAGTCCAGAA1480.28738422105283595No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCATTGTCAGTCCAGTA1400.2718499388337638No Hit
CCTATTTGCTACCCACGCTTTCGTACCTCAGCGTCAGTGATGGCCCAGCA1390.2699081535563797No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAA1360.26408279772422766No Hit
CCTGTTCGATACCCACGCTTTCGAGCTTCAGCGTCAGTTGCGCTACAGCA1360.26408279772422766No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAGCGTCAGTTATGCCCCGGCA1350.2621410124468436No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACTGTCCAGTA1340.2601992271694596No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAGAGCCCAGCA1330.25825744189207556No Hit
CCTATTTGCTCCCCACGCTTTCGTGTCTCAGCGTCAGTGATGGCCCAGCA1330.25825744189207556No Hit
CCTATTTGCTCCCCACGCTTTCGTGACTGAGCGTCGGTATCGGTCTGGCG1320.2563156566146915No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTGAACGTCAGTTACTGTCCAGCA1260.24466494495038738No Hit
CCGGTTCGCGCCCCTAGCTTTCGTCCCTCACCGTCAGAATCGTTCCAGTC1230.2388395891182353No Hit
CCCGTTCGCTCCCCTGGCTTTCGTGCCTCAGCGTCAGTTGTCGTCCAGAA1120.217479951067011No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG1110.21553816578962695No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACGGCCCAGTA1100.21359638051224294No Hit
CCTGTTTGCTACCCATGCTTTCGTGCCTCAGCGTCAGTTAGAGCCCAGTA1090.21165459523485894No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTGCCGTCCAGTA1040.2019456688479388No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACAGTCCAGTA1030.20000388357055476No Hit
CCTGTTTGATCCCCACGCTTTCGTGCCTCAGCGTCAATGCCTGTTTCGTG980.19029495718363465No Hit
CCTGTTTGCTCCCCACGCTCTCGCGCCTCAGCGTCAGTAACCGGCCAGAT970.18835317190625062No Hit
CCTGTTCGCTCCCCCAGCTTTCGCTCCTCAGCGTCAGTTGTGGCCCAGAA960.18641138662886658No Hit
CCTGTTTGCTACCCACGCTTTCGCATATCAGCGTCAGTATTTGCCCAGGA950.18446960135148255No Hit
CCTGTTTGCTCCCCACGCTTTCGTGTCTCAGCGTCAGTTGTGGTCCAGTA940.18252781607409851No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA850.16505174857764227No Hit
CCGGTTCGCGCCCCTGGCTTTCGTTACTCACCGTCAAGATCGTTCCAGTT750.145633895803802No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGTT750.145633895803802No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACCGTCCAGTA710.1378667546942659No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTCAGTCCAGAA700.1359249694168819No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGCA690.13398318413949786No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAGTGTCCAGCA670.1300996135847298No Hit
CCTGTTCGATACCCACGCTTTCGAGCTTCAGCGTCAGTTACGCTACAGCA660.12815782830734576No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGTA660.12815782830734576No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGCA660.12815782830734576No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCAGTTTCGGCCCAGAG640.12427425775257772No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTAGTGCCCAGTA620.12039068719780968No Hit
CCTGTTCGCTCCCCCCGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG570.11068176081088953No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTACCGTCCAGTA550.10679819025612147No Hit
CCTGTTCGATACCCGCACTTTCGAGCCTCAGCGTCAGTTGCGCTCCAGTG550.10679819025612147No Hit
CCTGTTTGCTACCCACGCTTTCGCCTATGAGCGTCAGTATTTGCCCAGTA550.10679819025612147No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACTGTCCAGCA540.10485640497873747No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGATGCCGGCCCAGGA540.10485640497873747No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAGGCCCAGTA540.10485640497873747No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGTC530.10291461970135343No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTA520.1009728344239694No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCTAGTG520.1009728344239694No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)