FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007703159

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007703159
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70608
Sequences flagged as poor quality0
Sequence length246-251
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA785911.130466802628597No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACGGATTAGTG710310.059766598685702No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA43876.213176977113076No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA31374.4428393383186044No Hit
CCTATTTGCTCCCCACGCTTTCGGGAATGAGCGTCAGTTGCGGCCCAGAC24323.4443689100385226No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG15642.215046453659642No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG14192.0096872875594833No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG13751.947371402673918No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGCA13681.9374575118966688No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG12651.7915816904600046No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGAA11461.6230455472467709No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGTA9941.4077724903693631No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA8481.2009970541581692No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA7050.9984704282800816No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA6170.8738386585089509No Hit
CCTGTTTGCTCCCCACGCTTTCGAGACTCAGCGTCAGTTATCGTCCAGTA5680.8044414230682075No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCATGTCCCAGAG5640.7987763426240653No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTCATGGCTTGGCA4870.6897235440743259No Hit
CCTATTTGCTCCCCACGCTTTCGCGAATGAGCGTCAGTTGCGGCCCAGAC4240.6004985270790846No Hit
CCTGTTTGATACCCACACTTTCGAGCCTGAACGTCAGTTACGGATTAGTG4200.5948334466349422No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGCA3900.552345343303875No Hit
CCTGTTTGCTCCCCACGCTTTCGAGACTCAACGTCAGTTACCGTCCAGTA3740.5296850215273057No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGTA3610.5112735100838431No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA3410.4829481078631317No Hit
CCTGTTTGATACCCACACTTTCGAGACTCAATGTCAGTTGCAGCTTAGCA3220.45603897575345576No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTGATCGTCCAGTA2800.39655563108996145No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAACAGTCCAGCA2660.3767278495354634No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA2170.3073306140947201No Hit
ACTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACGGATTAGTG2170.3073306140947201No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTTTAGTA2170.3073306140947201No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCATTGTCAGTCCAGTA2060.29175164287332883No Hit
CCTATTTGCTCCCCACGCTTTCGGGAATGAGCGTCAGTTGCGGCTCAGAC1630.23085202809879898No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTCCAGTA1570.22235440743258555No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAGCGTCAGTTACGGATTAGTG1450.20535916610015864No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA1450.20535916610015864No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTGCCGTCCAGTA1450.20535916610015864No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAG1440.20394289598912305No Hit
CCTGTTCGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTGGCGGCCCAGAA1410.19969408565601632No Hit
CCTGTTCGCTCCCCACGCTTTCGCTACTCAGCGTCAGTGACGGCCCAGAG1370.194029005211874No Hit
CCTGTTTGATACCCACACTTTCGCGCATGAACGTCAGTTACGGATTAGTG1350.19119646498980286No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA1320.18694765465669613No Hit
CCTGTTCGCTCCCCACGCTTTCGCTACTCAGCGTCAGTAACGGCCCAGAG1290.1826988443235894No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACCGTCCAGTA1260.17845003399048265No Hit
ACTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA1250.1770337638794471No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG1230.17420122365737592No Hit
CCTGTTTGCTCCCCACGCTTTCGAGACTCAGTGTCAGTTACAGTCCAGCA1210.17136868343530479No Hit
CCTGTTCGCTCCCCACGCTTTCGAGACTCAGCGTCAGTTACAGACCAGAG1160.1642873328801269No Hit
CCTGTTTGATCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA1100.15578971221391344No Hit
CCTATTTGCTCCCCACGCTTTCGAGAATGAGCGTCAGTTGCGGCCCAGAC1030.14587582143666442No Hit
CCTATTTGCTCCCCACGCTTTCGGGACTGAGCGTCAGTTGCGGCCCAGAC1030.14587582143666442No Hit
CCTATTTGCTCCCCACGCTTTCAGGAATGAGCGTCAGTTGCGGCCCAGAC960.13596193065941536No Hit
ACTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA960.13596193065941536No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAACGTCAGTTACCGTCCAGTA920.13029685021527304No Hit
CCTGTTTGATACCCACACTTTCGCGCCTCAATGTCAGTTGCAGCTTAGCA910.12888058010423747No Hit
ACTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA910.12888058010423747No Hit
CCTGTTTGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG880.12463176977113076No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAACGTCAGTTACGGATTAGTG840.11896668932698845No Hit
CCTGTTTGCTCCCCACGCTTTCGTGACTCAGTGTCAGTTTCAGTCCAGTA810.11471787899388171No Hit
CCTGTTTGCTCCCCACGCTTTCGAGACTCAACGTCAGTTACTGTCCAGTA800.11330160888284614No Hit
CCTGTTTGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG750.10622025832766825No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAG740.10480398821663267No Hit
CCTATTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA740.10480398821663267No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG730.1033877181055971No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTCATGGCTTTGCA720.10197144799456152No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA710.10055517788352594No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)