FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007703577

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007703577
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65225
Sequences flagged as poor quality0
Sequence length247-251
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA53828.25143733231123No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA35185.393637408968954No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGCA28414.355691835952472No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA22933.515523188961288No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA20843.1950939057110004No Hit
CCCTTTCGCTCCCCTGGCCTTCGTGCCTCAGCGTCAGTTAATGTCCAGGA18932.902261402836336No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACTGTCCAGAA18822.8853967037178996No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG18312.8072058259869683No Hit
CCTGTTCGATACCCACGCTTTCGAGCCTCAGCGTCAGTCGCGAGTTGGTA15892.4361824453813723No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA15742.4131851284016865No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA15572.387121502491376No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA14142.1678804139517056No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGTA13742.1065542353392104No Hit
CCTATTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTAACGGCCCAGCA12811.963970870065159No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTATCGTCCAGCA11921.8275201226523572No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG11881.8213875047911074No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA11191.7155998466845532No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTA10491.608279034112687No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA8811.350709083940207No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG8261.2663855883480262No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA7621.1682637025680338No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAG6941.064009198926792No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAACAGTCCAGCA6040.9260252970486776No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA5900.9045611345343043No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG5400.8279034112686854No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTGCTGTCCAGCA4040.6193944039862016No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA3880.5948639325412035No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAGAGCCCAGCA3860.5917976236105787No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAG2880.44154848600996555No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTCCAGAG2780.4262169413568417No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA2640.40475277884246835No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA2550.3909543886546569No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTGTCAGTCCAGAA2460.37715599846684555No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTTTAGTA2300.35262552702184746No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCATTGTCAGTCCAGTA2130.326561901111537No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTCCAGTT1940.29743196627060176No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGTA1900.29129934840935223No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACACTCCAGTG1830.28056726715216557No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTCAGTCCAGAA1820.27903411268685324No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTCCAGTA1750.2683020314296665No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTTGCGTCCCAGGG1700.2606362591031046No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTGCCGTCCAGTA1700.2606362591031046No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTGTCGTCCAGTA1680.25756995017247986No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAATGTCAGTTACAGGTTAGTA1590.24377155998466846No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGCA1480.22690686086623227No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTAGTGCCCAGTA1460.22384055193560753No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTAACAGTCTAGTG1300.19931008049060944No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACAGTCCAGTT1260.1931774626293599No Hit
CCTGTTTGATCCCCATGCTTTCGTGCCTCAGCGTCAGTATATGGCCAGAT1230.18857799923342278No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA1200.18397853583748563No Hit
CCTGTTTGCTACCCACACTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGTA1200.18397853583748563No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACTGTCCAGCA1170.1793790724415485No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTCAGCGTCAGTTACCGTCCAGGT1160.1778459179762361No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGTT1150.17631276351092373No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG1130.17324645458029897No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTAATAGCTTGGAA1080.16558068225373707No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCTAGTG1070.16404752778842469No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA1060.1625143733231123No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGCCGTCCAGTT1050.16098121885779992No Hit
CCTGTTTGATCCCCATGCTTTCGTGCCTCAGCGTCAGTTGACAGCCAGAT960.1471828286699885No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCTCAGTA920.14105021080873897No Hit
CCTGTTCGCTACCCACGCTTTCGTGCCTCAGCGTCAGTAATAGCTTGGAA900.1379839018781142No Hit
CCTGTTTGCTCCCCATGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA890.13645074741280183No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTGAACGTCAGTTACTGTCCAGCA890.13645074741280183No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTG880.13491759294748945No Hit
CCTGTTCGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTGGCGGCCCAGAA870.1333844384821771No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTCATGGCTTGGCA870.1333844384821771No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTCATCGTCCAGTA860.13185128401686472No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCATGTCCCAGAG830.12725182062092755No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCAGTTTCGGCCCAGAG790.12111920275967804No Hit
CCTGTTTTCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA790.12111920275967804No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTA750.11498658489842851No Hit
CCTGTTTGCTCCCCACGCTTTCGTACCTCAGTGTCAGTTACAGTCCAGAA720.11038712150249139No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGTA660.10118819471061709No Hit
CCTGTTTTCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGCA660.10118819471061709No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)