FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007703939

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007703939
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30545
Sequences flagged as poor quality0
Sequence length245-251
%GC53

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA23777.781961041086921No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA18506.05663774758553No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA15745.1530528728106075No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA14834.8551317727942385No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA14224.6554264200360125No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA13874.540841381568178No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA13624.4589949255197245No Hit
CCTGTTCGATCCCCGCACTTTCGTGCCTCAGCGTCAGTTGAGCGCCGGCA10543.4506465870027827No Hit
CCTGTTCGATACCCGCACCTTCGAGCATCAGCGTCAGTAATGCTGCGGCA5691.8628253396627925No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTGAACGTCAGTTACTGTCCAGCA5021.6434768374529383No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTCCAGAA4421.4470453429366508No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA3581.1720412506138485No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG3211.0509084956621377No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA2440.7988214110329023No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTCCAGTA2280.7464396791618924No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCGCATCCCAGGG2170.710427238500573No Hit
CCTATTTGCTCCCCACGCTTTCGGGACTGAGCGTCAGTTATGCGCCAGAT2070.6776886560811917No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA2050.6711409395973155No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTTTAGTA1940.6351284989359961No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACACTCCAGTG1890.6187592077263054No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA1800.5892944835488624No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTGCTGTCCAGCA1780.5827467670649861No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTGCCGTCCAGTA1620.5303650351939762No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA1570.5139957439842855No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAATGTCCAGAA1480.4845310198068424No Hit
CCTGTTTGCTCCCCACGCTTTCGTGTCTCAGCGTCAGTGGTCGTCCAGTA1450.474709445081028No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA1410.4616140121132755No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTGAGCGTCAGTAAGGCGCCGGTA1400.4583401538713373No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACAGTCCAGTA1340.43869700441970866No Hit
CCCATTTGCTACCCTGGCTTTCGTTCCTCAGTGTCAATTACGGCCCAGTA1330.43542314617777045No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTGTAGTCCAGAT1280.4190538549680799No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACAGTCCAGTT1270.4157799967261418No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTGACAGTCTAGTG1260.4125061384842036No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGCA1260.4125061384842036No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGTA1260.4125061384842036No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACTGTCCAGAA1240.4059584220003274No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGCGTCAGTTGAGCGCCGGTA1110.36339826485513177No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTCATGGCTTGGCA1090.3568505483712555No Hit
CCCGTTCGCTCCCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA1050.34375511540350306No Hit
CCTGTTCGATCCCCGCACTTTCGTGCCTGAGCGTCAGTATGGCGCCGGTA1000.3273858241938124No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAACAGTCCAGCA1000.3273858241938124No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG980.3208381077099362No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGTA970.31756424946799805No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAGAGCCCAGCA960.3142903912260599No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCTGTTTCACCCTAGCA930.30446881650024554No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGTA880.28809952529055494No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAATGTCAGTTACAGTCCAGTA860.2815518088066787No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGTA840.27500409232280243No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA840.27500409232280243No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGCGTCAGTTACAGTCCAGCA820.2684563758389262No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACAGTCCAGAA820.2684563758389262No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCATTGTCAGTCCAGTA820.2684563758389262No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTACCGTCCAGTA740.2422655099034212No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTG730.23899165166148306No Hit
CCTGTTTGATCCCCATGCTTTCGTGCCTCAGCGTCAGTTGACAGCCAGAT720.23571779341954496No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGTA680.22262236045179243No Hit
CCGGTTCGCGCCCCTAGCTTTCGTCCCTCACCGTCAGAATCGTTCCAGTC660.21607464396791617No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACGGTCCAGTA640.20952692748403995No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACCGTCCAGTA610.19970535275822557No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCTAGTG590.19315763627434931No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTAACAGTCTAGTG580.1898837780324112No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA550.1800622033065968No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTT540.17678834506465868No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTCATCGTCCAGTA540.17678834506465868No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGCGCCCAGTA520.17024062858078245No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA520.17024062858078245No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG510.16696677033884433No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAACGTCAGTTACAGTCCAGAA500.1636929120969062No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTCATGGCTTTGCA490.1604190538549681No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTGTCAGTCCAGAA470.15387133737109182No Hit
CCTGTTCGATACCCACGCTTTCGTGCTTCAGCGTCAGTTGGGCGCCGGTA460.1505974791291537No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGCA460.1505974791291537No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACGGCCCAGTA460.1505974791291537No Hit
CCCGTTCGCTCCCCTAGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA450.1473236208872156No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTATCGTCCAGCA440.14404976264527747No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGCGCCCAGTA440.14404976264527747No Hit
CCTGTTTGCTACCCACACTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGTA430.14077590440333934No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTAGTGCCCAGTA430.14077590440333934No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGCTGCCCAGTA410.1342281879194631No Hit
CCTGTTCGATCCCCGCACTTTCGTGCCTGAGCGTCAGTATGGAGCCGGTA400.13095432967752496No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTGCAGTCCAGTT400.13095432967752496No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAGGCCCAGCA390.12768047143558683No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGCTGTCCAGCA360.11785889670977248No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTACAGCCCAGCA340.11131118022589621No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGTA340.11131118022589621No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTGTCAGTCCAGAA320.10476346374201997No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTA320.10476346374201997No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGCTGCCCAGTA320.10476346374201997No Hit
CCTGTTTGCTACCCACGCTTTCGCGCCTCAGCGTCAGTTAAAGCCCAGCA310.10148960550008185No Hit
TCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA310.10148960550008185No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)