FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007704185

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007704185
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87831
Sequences flagged as poor quality0
Sequence length244-251
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA66267.544033427832996No Hit
CCTGTTCGATACCCGCGCTTTCGAGCTTCAGCGTCAGTTGCGCCCCAGTG63057.178558823194544No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA51315.841900923364188No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA43184.916259634980816No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAGCGTCAGTTGCACCCCGGAT42984.89348863157655No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA24602.8008334187245962No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA24482.7871708166820373No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGTA13871.5791690860857786No Hit
CCCGTTCGCTCCCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA10061.1453814712345298No Hit
CCTGTTTGATACCCACACTTTCGAGCATGAACGTCAGTTACGGCTTAGTG8330.9484122917876376No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA7670.8732679805535631No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGTA7420.8448042262982317No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTG7340.8356958249365258No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAACAGTCCAGCA7100.8083706208514079No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG6200.7059011055322153No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA5310.604570140383236No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA5290.6022930400428095No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA4970.5658594345959855No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTGCCGTCCAGTA4850.5521968325534264No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGAA4780.5442269813619337No Hit
CCTGTTCGATACCCGCACTTTCGAGCATGAGCGTCAGTTACGCACCCGTC4750.540811330851294No Hit
CCTATTTGCTCCCCACGCTTTCGGGACTGAGCGTCAGTTATGCGCCAGAT4520.5146246769363892No Hit
CCTGTTCGATACCCGCGCTTTCGAGCTTCAGCGTCAGTTACACTCCAGTA4510.5134861267661759No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGTA4480.5100704762555361No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAGTAGCGCGCTGGAA3700.42126356297890266No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCGCATCCCAGGG3610.41101661144698337No Hit
CCTGTTTGATCCCCATGCTTTCGTGCCTCAGTGTCAATATATAGCCAGAT3600.4098780612767702No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAACGTCAGTTACGGCTTAGTG3470.3950769090639979No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA3450.39279980872357134No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG3420.38938415821293165No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTA3420.38938415821293165No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA3370.3836914073618654No Hit
CCCATTTGCTACCCTGGCTTTCGTTCCTCAGCGTCAATTACGGCCCAGTA3350.38141430702143886No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAGTCGTCCAGTA2980.3392879507235486No Hit
CCTGTTCGATACCCACGCTTTCGAGCTTCAGCGTCAGTTGCGCTACAGCA2920.3324566497022691No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTCCAGTA2730.3108241964682174No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACCGTCCAGTA2560.29146884357459213No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTGAACGTCAGTTACTGTCCAGCA2510.2857760927235259No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAACGTCAGTTACCGTCCAGTA2320.2641436394894741No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA2270.25845088863840787No Hit
CCTGTTCGATACCCGCGCTTTCGCGCTTCAGCGTCAGTTGCGCCCCAGTG2240.25503523812776807No Hit
CCTGTTTGCTACCCACGCTTTCGCGCCTCAGCGTCAATACCGGTCCAGAC2240.25503523812776807No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCATTGTCAGTCCAGTA2210.2516195876171284No Hit
CCTGTTTGCTCCCCACGCTTTCGAGACTCAACGTCAGTTACCGTCCAGTA2070.23567988523414285No Hit
CCTGTTTGATCCCCATGCTTTCGTGCCTCAGCGTCAGTATATGGCCAGAT1960.22315583336179706No Hit
CCTGTTTGATACCCGCACTTTCGCGCCTCAGCGTCAGTTGCACCCCGGAT1900.21632453234051757No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTATCGTCCAGCA1880.2140474320000911No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTCAGTCCAGAA1870.21290888182987786No Hit
CCTGTTCGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTGGCGGCCCAGAA1830.2083546811490248No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCATGTCCCAGAG1670.1901378784256128No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTTTAGTA1630.18558367774475984No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTA1590.18102947706390682No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCTAGTA1580.17989092689369357No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA1560.17761382655326707No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGTA1550.17647527638305383No Hit
CCCATTTGCTACCCTGGCTTTCGTTCCTCAGCGTCAATCACGGCCCAGTA1540.17533672621284055No Hit
CCTGTTTGCTACCCACACTTTCGAGACTCAGCGTCAGTTGGTGCCCAGTA1510.1719210757022008No Hit
CCTGTTTGCTACCCACACTTTCGCGCCTCAGCGTCAGTTGGTGCCCAGTA1480.16850542519156106No Hit
CCTATTTGCTCCCCACGCTTTCGCGACTGAGCGTCAGTTATGCGCCAGAT1430.16281267434049482No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTT1420.16167412417028157No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAGCGTCAGTTGCACCCTGGAT1380.15711992348942855No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCTCAGTA1360.15484282314900205No Hit
CCTGTTTGATACCCGCACTTTCGAGACTCAGCGTCAGTTGCACCCCGGAT1350.1537042729787888No Hit
CCCATTTGCTACCCTGGCTTTCGTTCCTCAGTGTCAATTACGGCCCAGTA1320.15028862246814906No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCTAGTA1320.15028862246814906No Hit
CCTGTTCGATACCCGCGCTTTCGAGCCTCAGCGTCAGTTGCGCCCCAGTG1300.14801152212772256No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTTCTGTCCAGAA1270.14459587161708282No Hit
CCTGTTCGATACCCGCGCTTTCGAGCTTCAGCGTCAGTTGCGCCTCAGTG1250.1423187712766563No Hit
CCTGTTCGATACCCGCGCTTTCGAGCTTCAGCGTCAGTTGCGCCCTAGTG1220.13890312076601655No Hit
CCTGTTTGCTACCCATGCTTTCGAGACTCAGCGTCAGTTGGTGCCCAGTA1210.1377645705958033No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGTA1200.13662602042559005No Hit
CCTGTTTGATCCCCATGCTTTCGAGCCTCAGCGTCAATATACAGCCAGAT1120.12751761906388404No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAGCGTCAGTTGCACCTCGGAT1120.12751761906388404No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCAGTTTCGGCCCAGAG1100.12524051872345757No Hit
CCTGTTCGATACCCGCGCTCTCGAGCTTCAGCGTCAGTTGCGCCCCAGTG1100.12524051872345757No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGCA1050.1195477678723913No Hit
CCTGTTTGCTACCCATGCTTTCGCGCCTCAGCGTCAGTTGGTGCCCAGTA1050.1195477678723913No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAATGTCAGTTACAGGTTAGTA1040.11840921770217806No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAATCCCAGCA1040.11840921770217806No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA1040.11840921770217806No Hit
CCTATTTGCTCCCCACGCTTTCGTGCTTCAGTGTCAGAATCCAGACCAGA1020.11613211736175154No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCTCAGTA1010.11499356719153829No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCTAGTA1010.11499356719153829No Hit
CCTGTTTGCTCCCCACGCTTTCGATCCTCAACGTCAGTTACCGTCCAGTA990.1127164668511118No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGCTGTCCAGCA950.10816226617025879No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGCTGCCCAGTA940.10702371600004555No Hit
CCTGTTTGCTCCCCACGCTCTCGAGCCTCAACGTCAGTTACCGTCCAGTA920.10474661565961904No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG920.10474661565961904No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACAGTCCAGTT910.10360806548940578No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)