FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007704581

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007704581
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91575
Sequences flagged as poor quality0
Sequence length245-251
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA83299.095277095277094No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA60996.660114660114661No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA46315.057057057057057No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAATCCAGTA26842.930930930930931No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA19222.0988260988260987No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA18632.0343980343980346No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG13951.5233415233415233No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTG13091.4294294294294294No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGTA10701.1684411684411684No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACCGTCCAGTA9941.0854490854490855No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGCA7710.8419328419328419No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA7260.7927927927927928No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA6130.6693966693966694No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTGACAGTCTAGTG4950.5405405405405406No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG4770.5208845208845209No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA4720.5154245154245154No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG4640.5066885066885067No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA4170.45536445536445536No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA3970.43352443352443354No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAACAGTCCAGCA3930.42915642915642915No Hit
CCTGTTTGCTCTCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA3870.4226044226044226No Hit
CCTGTTTGCTCCCCACGCTTGCGAGCCTCAGCGTCAGTTATCGTCCAGTA3850.42042042042042044No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCTCAGTA3760.4105924105924106No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG3560.38875238875238877No Hit
CCTGTTTGCTACCCACACTTGCGAGCCTCAGCGTCAGTTGGTGCCCAGTA3170.34616434616434616No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGCGTCAGTTACAATCCAGTA3040.331968331968332No Hit
CCTATTTGCTCCCCACGCTTTCGGGACTGAGCGTCAGTTATGCGCCAGAT2960.3232323232323232No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTA2790.3046683046683047No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGTA2740.2992082992082992No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGTGTCAGTTGCAGCTTAGCA2670.2915642915642916No Hit
CCTGTTTGCTATCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA2650.28938028938028937No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA2550.27846027846027843No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA2520.2751842751842752No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTGCCGTCCAGTA2310.25225225225225223No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTATCGTCCAGCA2290.25006825006825006No Hit
CCTGTTTGCTCTCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA2280.24897624897624895No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA2090.22822822822822822No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA2010.21949221949221948No Hit
CCTGTTTGCTCCCCACGCTTGCGAGCCTCAACGTCAGTTACCGTCCAGTA1980.21621621621621623No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA1970.21512421512421515No Hit
CCTGGTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA1950.21294021294021295No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACACTCCAGTG1950.21294021294021295No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG1850.20202020202020202No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCGACGTCAGTTACCGTCCAGTA1670.18236418236418236No Hit
CCTGTTTGCGCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA1650.18018018018018017No Hit
CCTGTTTGATCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA1520.165984165984166No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA1510.16489216489216488No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGTA1500.16380016380016382No Hit
CCTGTTTGCTCCCCACGCTCTCGAGCCTCAGCGTCAGTTATCGTCCAGTA1480.1616161616161616No Hit
CCTGTTTGATATCCACACTTTCGAGCATCAGCGTCAGTTACAATCCAGTA1480.1616161616161616No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACTGTCCAGAA1420.15506415506415505No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCTCAGCA1380.1506961506961507No Hit
CCTGTTTGCTACCCACACTCTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA1330.14523614523614525No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCATGTCCCAGAG1260.1375921375921376No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA1230.13431613431613432No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCATTGTCAGTCCAGTA1210.13213213213213215No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA1200.13104013104013104No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCTGTTTCACCCTAGCA1200.13104013104013104No Hit
CCTGTTTGCTATCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA1170.12776412776412777No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG1170.12776412776412777No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTCCAGTA1160.12667212667212668No Hit
CCTGGTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA1150.12558012558012557No Hit
CCTGTTTGCGCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA1120.12230412230412231No Hit
CCTGTTTGATACCCACACTTGCGAGCATCAGCGTCAGTTACAATCCAGTA1120.12230412230412231No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCTAGTA1110.12121212121212122No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCAGTTTCGGCCCAGAG1060.11575211575211575No Hit
CCTGTTTGATACCCACACTTGCGAGCCTCAATGTCAGTTGCAGCTTAGCA1020.11138411138411138No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTCATCGTCCAGTA990.10810810810810811No Hit
CCTGTTTGCGACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA970.10592410592410592No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGCGCCCAGTA970.10592410592410592No Hit
CCTGGTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA930.10155610155610155No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)