FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007704587

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007704587
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85047
Sequences flagged as poor quality0
Sequence length245-251
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTACCCACGCTTTCGCATATGAGCGTCAGTATTTGCCCAGGA871210.243747574870365No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA33083.8896139781532564No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA22172.6067938904370527No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA17502.0576857502322246No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA15481.8201700236339908No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAGAGCCCAGCA15381.8084118193469494No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA13611.6002916034663188No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTGCAGTCCAGAA10471.2310839888532223No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGCA10291.2099192211365482No Hit
CCGGTTCGCGCCCCTAGCTTTCGTCCCTCACCGTCAGAATCGTTCCAGTC9421.1076228438392888No Hit
CCTGTTTGCTACCCACGCTTTCGCATCTGAGCGTCAGTATTTGCCCAGGA8350.9818100579679471No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGTA7920.9312497795336697No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA7860.9241948569614449No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTGTCAGTCCAGAA7740.9100850118169954No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGTA6570.7725140216586124No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA6540.7689865603724999No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTG6530.7678107399437958No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTCCAGTA6320.7431185109410091No Hit
CCTATTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTAACGGCCCAGCA5970.7019647959363646No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA5760.6772725669335778No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTATTGTCCAGAA5490.6455254153585664No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAA5180.6090749820687384No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATACCGGTCCAGGT5150.6055475207826261No Hit
CCTGTTTGCTACCCACGCTTTCGCATATGAGCGTCAGTATTTGCTCAGGA5030.5914376756381765No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA4660.5479323197761238No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGCTGTCCAGCA4590.5397015767751948No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAACAGTCCAGCA4170.4903171187696215No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACAGTCCAGAA4110.4832621961973967No Hit
CCTATTTGCTCCCCACGCTTTCGGGAATGAGCGTCAGTTGCGGCCCAGAC3990.46915235105294717No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA3980.46797653062424305No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA3830.4503392241936811No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG3540.4162404317612614No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACAGTCCAGTT3470.4080096887603325No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAGTAGCGCGCTGGAA3440.40448222747422014No Hit
CCTGTTTGCTATCCACGCTTTCGCATATGAGCGTCAGTATTTGCCCAGGA3330.39154820275847474No Hit
CCTATTTGCTCCCCACGCTTTCGTGCTTCAGTGTCAGAATCCAGACCAGA3330.39154820275847474No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTCCAGAA3020.35509776946864674No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTACAGTCCAGTA2990.3515703081825344No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTCAGCGTCAGTTACCGTCCAGGT2980.3503944877538302No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGTA2960.34804284689642195No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTA2920.34333956518160547No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCATTGTCAGTCCAGTA2870.33746046303808486No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTCCAGTT2860.3362846426093807No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACCGTCCAGTA2830.3327571813232683No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG2830.3327571813232683No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG2800.3292297200371559No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAGCGTCAGTGCTGCCCCGGCA2650.311592413606594No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACAGTCCAGTA2520.29630674803344037No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTAGTGCCCAGTA2480.2916034663186238No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTTCAGTCCAGAA2480.2916034663186238No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTAATGTCCAGTA2410.2833727233176949No Hit
CCTGTTTGCTACCCACGCTTGCGCATATGAGCGTCAGTATTTGCCCAGGA2390.2810210824602867No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGTA2320.27279033945935777No Hit
CCTGTTCGCTCCCCCGGCTCTCGCGCCTCAGCGTCAGTGCCCGCCCAGAT2260.265735416887133No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTTCTGTCCAGAA2250.2645595964584289No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTT2240.26338377602972474No Hit
CCTGTTCGCTCCCCATGCTTTCGCTCCTCAGCGTCAGTCACGGCCCAGTA2180.25632885345749995No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGCA2140.25162557174268346No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACTGTCCAGTA2130.25044975131397934No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCAGTTTCGGCCCAGAG2040.23986736745564216No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACACTCCAGTG2040.23986736745564216No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTCCAGTA2020.23751572659823392No Hit
CCCGTTCGCTGCCCTGGCTTTCGCACATGAGCGTCAGTTGTGTGCCAGGA1970.23163662445471328No Hit
CCTGTTTGCTCCCCACGCTTTCGTACCTCAGTGTCAGTTACAGTCCAGAA1950.22928498359730504No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA1900.2234058814537844No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTATCGTCCAGTA1890.22223006102508025No Hit
CCTGTTCGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACGGCCCAGTA1870.219878420167672No Hit
ACTGTTTGCTACCCACGCTTTCGCATATGAGCGTCAGTATTTGCCCAGGA1800.2116476771667431No Hit
CCTGTTCGCTCCCCCGGCTTTCGCGCCTCAGCGTCAGTGACGGCCCAGAG1780.20929603630933485No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA1710.20106529330840595No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTCATCGTCCAGTA1710.20106529330840595No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTGCCGTCCAGTA1600.18813126859266052No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTAATGGCTTGGCA1590.1869554481639564No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA1580.18577962773525228No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTGAACGTCAGTTACTGTCCAGCA1490.1751972438769151No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCGCATCCCAGGG1490.1751972438769151No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGCGTCAGTTGCACTCCGGAC1420.16696650087598622No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACGGTCCAGAA1390.16343903958987382No Hit
CCTGTTTGCTACCCACACTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGTA1370.16108739873246558No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTGCTGTCCAGCA1320.15520829658894494No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA1270.1493291944454243No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCTCAGCA1220.14345009230190364No Hit
CCCATTCGCTCCCCTAGCTTTCGTCTCTCAGTGTCAGTGTCGGCCCAGCA1210.14227427187319952No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAG1160.13639516972967888No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAGTGTCAGTAACAGTCCAGAA1160.13639516972967888No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTTTAGTA1150.13521934930097476No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGTT1140.1340435288722706No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA1130.1328677084435665No Hit
CCTGTTTGCTACCCACGCTTTCGCATATGAGCGTCAGTATTTGCCTAGGA1120.13169188801486237No Hit
CCTGTTCGATACCCACGCTTTCGAGCTTCAGCGTCAGTTGCGCTACAGCA1120.13169188801486237No Hit
CCTGTTTGCTCTCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA1070.12581278587134173No Hit
CCTATTTGCTCCCCACGCTTTCGTGCTTCAGTGTCAGCAACAGACCAGAC1070.12581278587134173No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTTTTGCCCCAGGG1060.1246369654426376No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAACCCAGTA1050.12346114501393347No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG1040.12228532458522935No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTAACAGTCCAGTA1040.12228532458522935No Hit
CATGTTTGCTACCCACGCTTTCGCATATGAGCGTCAGTATTTGCCCAGGA1010.11875786329911696No Hit
CCTGTTCGATACCCGCGCTTTCGAGCTTCAGCGTCAGTTACGCTCCCGCC1010.11875786329911696No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGTA1010.11875786329911696No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCAGTCGTGGCCCAGAG1000.11758204287041284No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAGTGTCCAGCA980.11523040201300457No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTGCAGTCCAGTT960.11287876115559632No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCTCAGTA920.1081754794407798No Hit
CCTGTTTGCTCCCCACGCTTGCGCGCCTCACCGTCAGTTGTCGTCCAGCA870.10229637729725916No Hit
CCTGTTTGCTACCCACGCTTTCGCATATGAGCGTCAGTATTTGCCCAGGT860.10112055686855503No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)