FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007705037

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007705037
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88001
Sequences flagged as poor quality0
Sequence length244-251
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA70668.029454210747605No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA66567.563550414199839No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA57136.491971682139976No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA49635.639708639674549No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG41454.710173748025591No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGTA30843.504505630617834No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA29713.3760979988863764No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG20522.331791684185407No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTA20272.303382916103226No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG13701.5568004909035125No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGTA10571.201122714514608No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTG10081.1454415290735331No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACCGTCCAGTA9081.0318064567448098No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA7760.8818081612708947No Hit
CCTATTTGCTCCCCACGCTTTCGGGACTGAGCGTCAGTTATGCGCCAGAT7670.8715810047613095No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGTGTCAGTTGCAGCTTAGCA7650.8693083033147351No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGTA6890.7829456483449052No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTGACAGTCTAGTG6870.7806729468983307No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTA5350.6079476369586709No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAACAGTCCAGCA4890.5556755036874581No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA4770.5420392950080113No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA4340.4931762139066601No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG3670.4170407154464154No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTTATGTCCCAGGA3620.4113589618299792No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA3560.4045408574902558No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA3380.38408654447108553No Hit
CCTGTTTGCTCTCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA3210.3647685821752026No Hit
CCTGTTTGCTATCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA3050.3465869706026068No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCTCAGTA2920.33181441119987276No Hit
CCTGTTTGATATCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA2590.29431483733139396No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCTCAGAG2500.28408768082180885No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTGAACGTCAGTTACTGTCCAGCA2490.2829513300985216No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTGCCGTCCAGTA2470.28067862865194715No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA2450.27840592720537266No Hit
GCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA2410.2738605243122237No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTCCAGAG2410.2738605243122237No Hit
GCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA2310.26249701707935136No Hit
GCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA2250.25567891273962795No Hit
GCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG2040.23181554755059602No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA1980.22499744321087262No Hit
CCTGTTTGCTCTCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA1890.21477028670128748No Hit
CCTGTTTGCTCCCCACGCTTTCGTGACTCAGCGTCAGTAACAGTCCAGTA1860.21136123453142577No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTGAACGTCAGTTACTGTCCAGTA1790.20340677946841515No Hit
CCTGTTCGCTCTCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG1760.19999772729855345No Hit
GCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA1750.1988613765752662No Hit
CCTGTTTGATACCCACACTTGCGAGCCTCAATGTCAGTTGCAGCTTAGCA1650.18749786934239382No Hit
CCTGTTTGCTACCCACACTTGCGAGCCTCAGCGTCAGTTGGTGCCCAGTA1560.1772707128328087No Hit
GCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA1430.16249815343007465No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTGGTCGTCCAGTA1420.16136180270678743No Hit
CCTGTTTGCTCTCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGTA1410.1602254519835002No Hit
CCTGTTTGCTCCCCACGCTTGCGAGCCTCAACGTCAGTTACCGTCCAGTA1400.15908910126021295No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCATTGTCAGTCCAGTA1390.1579527505369257No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCGACGTCAGTTACCGTCCAGTA1370.15568004909035124No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCTCAGAG1370.15568004909035124No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAG1340.15227099692048954No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCTCAGTA1320.14999829547391508No Hit
CCTGTTTGCTATCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA1310.14886194475062783No Hit
GCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG1200.13636208679446826No Hit
GCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGTA1180.13408938534789377No Hit
CCTGTTTGCTCCCCACGCTTGCGAGCCTCAGCGTCAGTTATCGTCCAGTA1180.13408938534789377No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGCG1090.12386222883830865No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGTCGGCCCAGAG1050.11931682594515973No Hit
CCTGTTCGCTCCCCACGCTTGCGCTCCTCAGCGTCAGTGACGGCCCAGAG1000.11363507232872354No Hit
CCTGTTCGCTCTCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG990.11249872160543631No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG980.11136237088214906No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTCCAGTT960.10908966943557459No Hit
CCTGTTTGCTCTCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTA890.10113521437256395No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTCATCGTCCAGTA890.10113521437256395No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)