FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007706087

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007706087
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60265
Sequences flagged as poor quality0
Sequence length246-251
%GC54

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA815313.528582095743799No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA752812.491495893138637No Hit
CCTGTTTGCTACCCACGCTTTCGTGCATCAGCGTCAGTTAAGGCCCAGCA44297.34920766614121No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG17742.9436654774744877No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACTGTCCAGAA17552.912138056915291No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA16142.678171409607567No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA13852.2981830249730355No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTA10671.77051356508753No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA10231.6975026964241267No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAACAGTCCAGCA9621.5962830830498633No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG6781.1250311125860781No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA6761.1217124367377418No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA5950.9873060648801129No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA5610.9308885754583921No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGTA5350.8877457894300174No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA5050.8379656517049697No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA4500.7467020658757156No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTT4470.7417240521032108No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCATTGTCAGTCCAGTA4250.7052186177715092No Hit
CCCTTTCGCTCCCCTGGCCTTCGTGCCTCAGCGTCAGTTAATGTCCAGGA3850.6388451008047789No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGTA3740.6205923836389281No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG3650.6056583423214137No Hit
CCTGTTCGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTGGCGGCCCAGAA2910.4828673359329627No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTATCGTCCAGCA2490.41317514311789594No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCTCAGTA2400.39824110180038164No Hit
ACTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA2330.38662573633120384No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTGCTGTCCAGCA2310.38330706048286733No Hit
CCTATTTGCTACCCACGCTTTCGTACCTCAGCGTCAGTTACAGCCCAGCA2230.37003235708952126No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTGCCGTCCAGTA2200.3650543433170165No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA2070.34348295030282916No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAGGCCCAGCA1960.32523023313697835No Hit
ACTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA1850.30697751597112755No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGCCCAGTA1730.2870654608811084No Hit
CCTGTTTGCTACCCATGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGTA1720.2854061229569402No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGTA1570.2605160540944163No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTCATCGTCCAGTA1560.2588567161702481No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGCTGTCCAGCA1510.2505600265494068No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACTGTCCAGCA1490.24724135070107026No Hit
CCTGTTTGCTACCCACACTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGTA1440.23894466108022896No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACCGTCCAGTA1420.2356259852318925No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGTA1410.2339666473077242No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA1400.23230730938355598No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTG1350.2240106197627147No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGCGCCCAGTA1330.22069194391437816No Hit
CCTGTTTGCTACCCACGCTTTCGTGCATCAGCGTCAGTTAAGGCTCAGCA1280.21239525429353687No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGCCGTCCAGTT1170.19414253712768606No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGTA1170.19414253712768606No Hit
CCTGTTTGATCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA1160.19248319920351778No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAA1110.18418650958267652No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCAGTCGTGGCCCAGAA1040.1725711441134987No Hit
CCTGTTTGATACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA1010.16759313034099393No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA1000.16593379241682568No Hit
ACTGTTTGCTACCCACGCTTTCGTGCATCAGCGTCAGTTAAGGCCCAGCA960.15929644072015264No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAG940.15597776487181614No Hit
CCTATTTGCTCCCCACGCTTTCGGGAATGAGCGTCAGTTACAGGCCAGAC930.15431842694764789No Hit
CCTGTTTGCTCCCCACACTTTCGTGTCTCAGCGTCAGTTGTCGTCCAGTA910.15099975109931138No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG910.15099975109931138No Hit
CCTGTTTGCTACCCACGCTTTCGTGCATCAGCGTCAGTTACGGCCCAGCA910.15099975109931138No Hit
CCTATTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTAATGGCCCAGTA900.14934041317514313No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTAATAGCTTGGAA880.1460217373268066No Hit
CCTGTTTGCTCCCCACGCTTTCGTACCTCAGCGTCAGTTACAGTCCAGAA870.14436239940263834No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCACTGTCCAGTA840.13938438563013356No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTAGTGCCCAGTA800.13274703393346055No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA780.12942835808512404No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAACGTCAGTTACAGTCCAGAA770.1277690201609558No Hit
CCTGTTTGCTACCCACGCTTTCGCGCATCAGCGTCAGTTAAGGCCCAGCA760.1261096822367875No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGCA740.12279100638845102No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTCCAGCA700.11615365469177799No Hit
CCTGTTTGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA700.11615365469177799No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTGTCGTCCAGTA670.11117564091927322No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)