FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007706201

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007706201
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62193
Sequences flagged as poor quality0
Sequence length245-251
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTACCCACGCTTTCGAACCTCAGCGTCAGTTACAGACCAGAG668410.747190198253822No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA46067.405978164745229No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTGACAGTCTAGTG44797.201775119386427No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG26454.252890196645924No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA26354.236811216696413No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA23933.8476999019182223No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA21063.3862331773672283No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA19873.1948933159680353No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG16082.5854999758815302No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA15422.479378708214751No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA14012.252665090926632No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG13882.231762416992266No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCATTGTCAGTCCAGTA9751.5677005450774204No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTTTAGTA9591.5419741771582012No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG8991.445500297461129No Hit
CCTATTTGCTCCCCACGCTTTCGGGAATGAGCGTCAGTTGCGGCCCAGAC6731.082115350602158No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTATCGTCCAGTA5850.9406203270464523No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG4050.6511986879552362No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTTGCGTCCCAGGG3820.6142170340713584No Hit
CCTATTTGCTCCCCACGCTTTCGTACTTCAGCGTCAGTTTCAGACCAGGC3070.4936246844500185No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTCAGCGTCAGTTACCGTCCAGGT2710.43574035663177524No Hit
CCTGTTTGATCCCCACGCTTTCGTGCATCAGCGTCAGTCATGGCTTGGCA2370.3810718248034345No Hit
CCTGTTTTCTACCCACGCTTTCGAACCTCAGCGTCAGTTACAGACCAGAG2320.3730323348286785No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCATGTCCCAGAG2020.3247953949801425No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA1990.3199717009952889No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGTGTCAGTGACAGTCTAGTG1920.30871641503063046No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGAA1790.2878137410962649No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTCCAGAG1630.26208737317704567No Hit
CCTGTTTTATACCCACACTTTCGAGCATCAGTGTCAGTGACAGTCTAGTG1230.19777145337899765No Hit
CCTGTTTTATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA1120.18008457543453443No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGCGTCAGTTACAGTCCAGCA1110.17847667743958323No Hit
CCTGTTCTCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG1080.17365298345472963No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA1050.16882928946987602No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCTCAGAG1010.16239769749007124No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGCTGTCCAGCA1010.16239769749007124No Hit
CCTGTTTGCTACCCACGCTTTCGAACCTCAGCGTCAGTTACAGACTAGAG960.15435820751531523No Hit
CCTGTTTTCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA930.14953451353046163No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCATTTGCAGCTTAGCA910.14631871754055922No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACTGTCCAGCA900.14471081954560802No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCATGCCCCAGAG900.14471081954560802No Hit
CCTGTTTTCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA850.136671329570852No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTATCGGCCCAGAG830.1334555335809496No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGTTCTCGGCCCAGAG800.12863183959609603No Hit
CCTGTTTGATACCCACGCTTTCGAACCTCAGCGTCAGTTACAGACCAGAG780.12541604360619363No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCATTGACAGTCTAGTG720.11576865563648642No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTGTCAGTCTAGTG700.11255285964658403No Hit
CCTGTTTTCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA670.10772916566173041No Hit
CCTGTTCTCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG660.10612126766677923No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)