FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007706313

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007706313
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52956
Sequences flagged as poor quality0
Sequence length247-251
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTCGATACCCACGCTTTCGAGCTTCAGCGTCAGTTGCGCTACAGCA774414.623460986479342No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA52609.932774378729512No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA49789.400256816980136No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA21364.033537276229322No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA17993.3971599063373366No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGCGTCAGTTGCACTCCGGAC13282.5077422766069946No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG11602.1904977717350254No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCGCATCCCAGGG11402.1527305687740768No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA10612.003550117078329No Hit
CCCGTTCGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA7921.495581237253569No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG7261.3709494674824383No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGCA6851.2935267014124934No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTTGTCCAGGG6761.2765314600800663No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA6071.1462346098647933No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA5921.117909207644082No Hit
CCTATTTGCTCCCCACGCTTTCGGGAATGAGCGTCAGTTGCGGCCCAGAC5290.9989425183170935No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGCA4870.9196313920991012No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG4820.9101895913588639No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTA3990.7534556990709268No Hit
CCTGTTCGATACCCACGCTTTCGAGCCTCAGCGTCAGTCGCGAGTTGGTA3860.7289070171463101No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGTA3850.7270186569982626No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG3830.7232419367021679No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACCGTCCAGTA3720.7024699750736461No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA3630.6854747337412191No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAACAGTCCAGCA3540.6684794924087922No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA3520.6647027721126973No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAGGCCCAGTA3430.6477075307802704No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACTGTCCAGAA2760.5211874008610922No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAGCGTCAGTGCTGCCCCGGCA2720.5136339602689025No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTATCGTCCAGCA2590.48908527834428583No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA2400.45320643553138457No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTG2170.4097741521262935No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAGCGTCAGTTATGCCCCGGCA2160.4078857919782461No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA2050.3871138303497243No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG1980.37389530931339227No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG1940.3663418687212025No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTCCAGAG1940.3663418687212025No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA1890.3569000679809653No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA1870.35312334768487047No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA1780.33612810635244356No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTCCAGTT1730.32668630561220635No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAG1700.32102122516806403No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA1500.2832540222071153No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA1480.2794773019110205No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGTA1470.27758894176297305No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGAA1390.2624820605785936No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGCTGTCCAGCA1340.2530402598383564No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGTA1330.25115189969030893No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTGCCGTCCAGTA1260.2379333786539769No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG1210.22849157791373972No Hit
CCTGTTCGATACCCACGCTTTCGAGCCTCAGCGTCAGTTGCGCTACAGCA1190.22471485761764481No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTGAACGTCAGTTACTGTCCAGCA1090.2058312561371705No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTTTAGTA990.18694765465669613No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAGAGCCCAGCA980.1850592945086487No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAATCCAGTA950.1793942140645064No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGTA840.1586222524359846No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTACAGTCCAGTA840.1586222524359846No Hit
CCTGTTTGCTACCCACACTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGTA830.15673389228793716No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTAACAGTCTAGTG820.15484553213988972No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTCAGTCCAGAA820.15484553213988972No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCATGTCCCAGAG810.15295717199184228No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCATTGTCAGTCCAGTA730.1378502908074628No Hit
ACTGTTCGATACCCACGCTTTCGAGCTTCAGCGTCAGTTGCGCTACAGCA720.13596193065941536No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAGCGTCAGTGCTGCCCCCGCA680.12840849006722563No Hit
CCTGTTTGCTACCCATGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGTA680.12840849006722563No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGTA670.1265201299191782No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATACCGGTCCAGGT620.117078329178941No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTCCAGTA560.10574816829065639No Hit
CCTGTTTGCTACCCATGCTTTCGTGCTTCAGCGTCAGTTAAAGCCCAGTA530.10008308784651408No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)