FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007706529

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007706529
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69608
Sequences flagged as poor quality0
Sequence length246-251
%GC54

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA852112.241409033444432No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA63969.188599011607861No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA58108.3467417538214No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA32194.62446845190208No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA19942.8646132628433514No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA16172.3230088495575223No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA14792.124755775198253No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAACAGTCCAGCA14362.0629812665210894No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG13361.9193196184346628No Hit
CCTATTTGCTCCCCACGCTTTCGTGCTTCAGTGTCAGCAACAGACCAGAC13331.9150097689920698No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGTA12801.8388690955062637No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA11281.6205033904148947No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGCGTCAGTTGCACTCCGGAC10621.5256867026778531No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA10241.471095276405011No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA8091.1622227330191932No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTGAACGTCAGTTACTGTCCAGCA7431.0674060452821514No Hit
CCTATTTGCTCCCCACGCTTTCGGGACTGAGCGTCAGTTATGCGCCAGAT5990.8605332720376968No Hit
CCTGTTCGATACCCACGCTTTCGAGCTTCAGCGTCAGTTGCGCTACAGCA5600.8045052292839904No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG5210.7484771865302838No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG5160.7412941041259625No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGCA4930.7082519250660844No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCTAGTG4830.6938857602574418No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTGCCGTCCAGTA4670.6708998965636134No Hit
CCTGTTCGATACCCGCGCTTTCGAGCTTCAGCGTCAGTTACGCTCCCGCC4140.5947592230778072No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA3760.540167796804965No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA3760.540167796804965No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGCTGTCCAGCA3500.502815768302494No Hit
CCTATTCGCTCCCCACGCTTTCGTGCTTCAGTGTCAGAATCCAGACCAGA3400.48844960349385125No Hit
CCTGTTTGCTACCCACACTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGTA3110.4467877255487875No Hit
CCTGTTTGCTACCCACGCTTTCGAACCTCAGCGTCAGTTACAGACCAGAG3080.4424778761061947No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTG3060.4396046431444662No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGTGTCAGTCATGGCTTGGCA2920.4194920124123664No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG2890.41518216296977356No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTACGCTACTGTA2860.41087231352718073No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACCGTCCAGTA2740.39363291575680953No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAGTCGTCCAGTA2600.3735202850247098No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTT2540.3649005861395242No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGTA2440.35053442133088153No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCTAGAA2140.3074359269049534No Hit
CCTATTTGCTCCCCACGCTTTCGTGCTTCAGTGTCAGAATCCAGACCAGA2140.3074359269049534No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGCA2030.2916331456154465No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAGGCCCAGTA1770.2542811171129755No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTATCGTCCAGCA1620.2327318699000115No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTACCCAGTA1580.22698540397655442No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTCAGCGTCAGTTACCGTCCAGGT1430.20543615676359037No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGTGTCAGTCATGGCTTTGCA1350.19394322491667623No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA1270.1824502930697621No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCATTGTCAGTCCAGTA1250.17957706010803356No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATACCGGTCCAGGT1210.1738305941845765No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTCCAGAG1130.16233766233766234No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCAGTTTCGGCCCAGAG1050.15084473049074817No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAATCGCCCCTTCGTG1020.14653488104815537No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGCTGTCCAGTT1020.14653488104815537No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCTCAGTA940.13504194920124124No Hit
CCTGTTCGCTCCCCCAGCTTTCGCTCCTCAGCGTCAGTCGAGGCCCAGAA890.1278588667969199No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAACGTCAGTTACCGTCCAGTA880.12642225031605564No Hit
CCTGTTTGCTCCCCACGCTTTCGTGACTCAGCGTCAGTAACAGTCCAGTA870.12498563383519135No Hit
CCTGTTCGATACCCACGCTTTCGAGCTTCAGCGTCAGTTACGCTACAGCA850.12211240087346281No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTTGCGTCCCAGGG800.11492931846914148No Hit
CCTGTTCGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGCAGACCAGAG790.11349270198827721No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGTGTCAGTTGGTGCCCAGTA730.1048730031030916No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTACAGCCCAGCA700.1005631536604988No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA700.1005631536604988No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)