FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007706829

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007706829
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92685
Sequences flagged as poor quality0
Sequence length247-251
%GC54

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA75768.173922425419432No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA71747.740195285105465No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA68767.418676161191132No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG34693.742784700868533No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA33523.616550682418946No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA30973.3414252575929217No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGTA24502.6433619247990503No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG22262.4016831202459943No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAATCCAGTA19402.0931110751470032No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG19282.080163996331661No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA15111.6302530074985166No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA13061.4090737444030859No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGCA11111.1986837136537736No Hit
CCTATTTGCTCCCCACGCTTTCGGGACTGAGCGTCAGTTATGCGCCAGAT6990.7541673409936883No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTG6870.741220262178346No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAACGTCAGTTATTGTCCAGAA6640.7164050277822732No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAACAGTCCAGCA5970.6441171710632788No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA5730.6182230134325943No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG5580.6020391649134164No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTTATGTCCCAGGA5410.5836974699250148No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA5340.5761450072827319No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA4570.49306791821761886No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA4450.4801208394022765No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGTA4420.47688406969844094No Hit
CCTGTTTGCTCCCCACGCTTTCGAGACTCAACGTCAGTTACCGTCCAGTA4150.4477531423639208No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCTAGTA3880.4186222150294006No Hit
CCTGTTTGCTACCCACACTTTCGAGACTCAGCGTCAGTTGGTGCCCAGTA3650.3938069806333279No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA3470.37438636241031453No Hit
CCTGTTTGCTCCCCACGCTTTCGAGACTCAGCGTCAGTTATCGTCCAGTA3210.3463343583104062No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTAACAGTCTAGTG3170.3420186653719588No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTA3000.32367697038355725No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCTGTTTCACCCTAGCA2930.3161245077412742No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTGCCGTCCAGTA2630.28375681070291847No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAACGTCAGTTACCGTCCAGTA2610.28159896423369474No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCATTGTCAGTCCAGTA2600.28052004099908295No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCTAGTA2590.27944111776447106No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA2370.2557048066030102No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG2310.24923126719533906No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACCGTCCAGTA2240.24167880455305607No Hit
CCTGTTTGCTCCCCACGCTCTCGAGCCTCAGCGTCAGTTATCGTCCAGTA2190.23628418837999676No Hit
CCTGTTTGCTACCCACACTTTCGCGCCTCAGCGTCAGTTGGTGCCCAGTA2170.23412634191077303No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCATGTCCCAGAG2170.23412634191077303No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTGCTGTCCAGCA2080.22441603279926634No Hit
CCTGTTTGCTACCCACGCTTTCGTGACTCAGCGTCAGTTAAAGCCCAGCA1980.21362680045314775No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTCCAGTA1980.21362680045314775No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTATCGGCCCAGAG1910.20607433781086476No Hit
CCTGTTTGATACCCACACTTTCGAGACTCAATGTCAGTTGCAGCTTAGCA1850.19960079840319359No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCTCAGTA1830.1974429519339699No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCTAGTA1810.1952851054647462No Hit
CCTGTTTGCTACCCATGCTTTCGAGTCTCAGCGTCAGTTGGTGCCCAGTA1770.19096941252629873No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGATGCCCAGTA1770.19096941252629873No Hit
CCTATTTGCTCCCCACGCTTTCGCGACTGAGCGTCAGTTATGCGCCAGAT1760.1898904892916869No Hit
CCTGTTCGCTCCCCACGCTTTCGCTACTCAGCGTCAGTAACGGCCCAGAG1580.17046987106867348No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCCGATATAGATTTGTA1540.16615417813022604No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGTCCCAGAG1540.16615417813022604No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA1520.16399633166100233No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGTGTCAGTTGCAGTCCAGTG1490.16075956195716676No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACATTCCAGCA1450.15644386901871932No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTGACAGTCTAGTG1440.15536494578410745No Hit
CCTGTTTGCTCCCCACGCTTTCGTGACTCAGTGTCAGTTTCAGTCCAGTA1420.15320709931488374No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG1370.14781248314182446No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGCG1350.14565463667260073No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTCAGTCCAGAA1290.1391810972649296No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAACGCCCAGCA1290.1391810972649296No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGTCCCAGAG1280.13810217403031774No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA1270.13702325079570588No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGCGTCAGTTACAATCCAGTA1270.13702325079570588No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTT1260.135944327561094No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCTCAGAG1240.1337864810918703No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCTAGCA1230.13270755785725846No Hit
CCTGTTCGCTCCCCACGCTTTCGCTACTCAGCGTCAGTGACGGCCCAGAG1210.13054971138803476No Hit
CCTGTTTGATACCCACACTTTCGCGCCTCAATGTCAGTTGCAGCTTAGCA1180.12731294168419918No Hit
CCTGTTTGCTACCCACGCTTTCGCGCCTCAGCGTCAGTTAAAGCCCAGCA1150.1240761719803636No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGCTGTCCAGCA1120.12083940227652804No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTGAACGTCAGTTACTGTCCAGCA1100.11868155580730431No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA1090.11760263257269245No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCTCAGCA1080.11652370933808058No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGCTGCCCAGTA1080.11652370933808058No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGCGTCAGTTACAGTCCAGCA1070.11544478610346874No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTCAGCGTCAGTTACCGTCCAGGT1050.11328693963424503No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTACAGCCCAGCA1040.11220801639963317No Hit
CCTGTTTGCTACCCACACTCTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA1010.10897124669579761No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTTCAGTCCAGTA980.10573447699196202No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTTCCCAGTA980.10573447699196202No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTGAACGTCAGTTACTGTCCAGTA970.10465555375735015No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)