FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007706917

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007706917
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72059
Sequences flagged as poor quality0
Sequence length246-251
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA899312.480051069262688No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA26143.6275829528580745No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTG23573.2709307650675137No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA21763.01974770674031No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA21522.9864416658571447No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAATCCAGTA21342.961462135194771No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA19462.7005648149433106No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTTCGTCCAGAA17912.485463300906202No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA13781.9123218473750674No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG13401.8595872826433892No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA13341.8512607724225982No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAACAGTCCAGCA12931.7943629525805243No Hit
CCTATTTGCTCCCCACGCTTTCGGGACTGAGCGTCAGTTATGCGCCAGAT8551.1865277064627597No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAACGTCAGATATAGTTTGGTA8411.1670991826142467No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGTA8261.1462829070622684No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA8211.1393441485449423No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA7791.0810585769994032No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTGAGCGTCAGTTATGTCCCAGGA6340.879834579996947No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGCCGTCCAGTT5820.8076714914167556No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGTA5430.7535491749816123No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG4330.6008964876004386No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGTA4220.5856312188623212No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGCA4120.571753701827669No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA3620.5023661166544082No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAATGTCAGTTACAGGTTAGTA3490.4843253445093604No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA3430.4759988342885691No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA3230.4482438002192648No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAACGTCAGTTACCGTCCAGTA3160.43852953829500824No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTT3100.4302030280742169No Hit
CCTGTTTGCTCCCCACGCTTTCGAGACTCAACGTCAGTTACCGTCCAGTA2770.3844072218598648No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACTGTCCAGCA2530.3511011809766997No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGAA2260.3136318849831388No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTGAACGTCAGTTACTGTCCAGCA2250.3122441332796736No Hit
CCTATTTGCTCCCCACGCTTTCGCGACTGAGCGTCAGTTATGCGCCAGAT2140.2969788645415562No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAGCGTCAGTTATGCCCCGGCA2040.2831013475069041No Hit
CCTGTTCGATACCCACGCTTTCGTGCCTCAGCGTCTGTTTCACCCTAGCA1950.2706115821757171No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCTAGTA1950.2706115821757171No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGCA1930.2678360787687867No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACCGTCCAGTA1900.26367282365839106No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGCTGTCCAGCA1810.2511830583272041No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGCGTCAGTTACAATCCAGTA1690.2345300378856215No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAAGCCCAGCA1520.21093825892671286No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTAACAGTCTAGTG1400.19428523848513024No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAATCCCAGCA1390.19289748678166505No Hit
CCTGTTTGATCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA1390.19289748678166505No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA1350.18734647996780418No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGCGTCAGTTACAGTCCAGCA1350.18734647996780418No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTCCAGAG1340.18595872826433893No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCATTTACCGTCCAGTA1330.18457097656087373No Hit
CCTGTTTGCTCCCCACGCTCTCGAGCCTCAACGTCAGTTACCGTCCAGTA1240.17208121122968678No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTATCGTCCAGTA1220.16930570782275636No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCTAGCA1160.16097919760196505No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTA1140.15820369419503463No Hit
CCTGTTTGCTCCCCACACTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGAA1100.15265268738117377No Hit
CCTGTTTGATCCCCACGCTTTCGTGCTTCAGTGTCAGTTATGGTTTAGTA1100.15265268738117377No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTATCGGCCCAGAG1040.14432617716038246No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTCAGCGTCAGTTACCGTCCAGGT1010.14016292204998682No Hit
CCTGTTTGCTACCCACGCTTTCGCGCCTCAGCGTCAGTTAAAGCCCAGCA1000.1387751703465216No Hit
CCTGTTTGCTCCCCACGCTTTCGAGACTCAGTGTCAGTTACAGTCCAGTG960.13322416353266073No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTATCGTCCAGTA950.13183641182919553No Hit
CCTGTTCGCTCCCCTAGCTTTCGCGCCTCAGCGTCAGTTGCGGCCCAGCA940.13044866012573028No Hit
CCTGTTTGATACCCACACTTTCGCGCCTCAATGTCAGTTGCAGCTTAGCA910.12628540501533467No Hit
CCTGTTTGATACCCACACTTTCGCGCATCAGCGTCAGTTACAATCCAGTA900.12489765331186943No Hit
CCTGTTTGATACCCACACTTTCGCGCATCAGCGTCAGTTACAGTCCAGCA850.11795889479454336No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTAGTGCCCAGTA850.11795889479454336No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAATCTAGTA850.11795889479454336No Hit
CCTATTTGCTCCCCACGCTTTCAGGACTGAGCGTCAGTTATGCGCCAGAT790.10963238457375206No Hit
CCTGTTTGATACCCACACTTTCGAGACTCAATGTCAGTTGCAGCTTAGCA770.10685688116682164No Hit
CCTGTTTGCTCCCCACGCTTTCGATCCTCAACGTCAGTTACCGTCCAGTA750.1040813777598912No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTACAGCCCAGCA740.10269362605642599No Hit
CCTGTTTGCTCCCCACGCTTTCGAGACTCAGCGTCAGTTATCGTCCAGTA740.10269362605642599No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGTCCAGTG740.10269362605642599No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA730.10130587435296078No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)