FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007707040

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007707040
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93887
Sequences flagged as poor quality0
Sequence length248-251
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGTAGGCGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGAGCGCA3248834.60329971135514No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA2020021.51522575010385No Hit
TACAGAGGTCTCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGTGCGTA54015.752660112688657No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA53275.6738419589506535No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA31983.4062223737045603No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA29243.1143821828368146No Hit
TACGTAGGGTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA24272.5850224205694077No Hit
TACGTAGGGGGCGAGCGTTATCCGGATTCATTGGGCGTAAAGCGCGCGTA20752.2101036352210635No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA17941.9108076730537775No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA13811.470917166380862No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGAGGGAGCA8820.9394271837421581No Hit
AACGTAGGGTGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA4670.4974064566979454No Hit
TACGTAGGCGGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGGGAGCGCA4100.4366951761159692No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA2920.31101217420942195No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA1770.18852450285982084No Hit
TACGTAGGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA1400.14911542599081876No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGAGCGCA1360.14485498524822393No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGCGTAAAGGGAGCGTA1320.1405945445056291No Hit
TACGGAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA1260.1342038833917369No Hit
TACGGAGGCGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGAGCGCA1200.12781322227784464No Hit
TACGTAGGCGGCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGAGCGCA1150.12248767134960112No Hit
TACGTAGGCGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA1120.119292340792655No Hit
TACGTAGGCGGCAAGCGTTGTCCGGAATTATTGGGCGTAAGGGGAGCGCA1060.11290167967876277No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCACGCA1060.11290167967876277No Hit
TACGTAGGCGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGAGCGCA1040.11077145930746535No Hit
TACGTAGGGGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGGGAGCGCA1000.10651101856487054No Hit
TACGTAGGCGGCAAGCGCTGTCCGGAATTATTGGGCGTAAAGGGAGCGCA970.10331568800792443No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA950.101185467636627No Hit
TACGTAGGCGGCAAGCGTTGTCCGGAGTTATTGGGCGTAAAGGGAGCGCA940.1001203574509783No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)