FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007707331

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007707331
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60329
Sequences flagged as poor quality0
Sequence length245-251
%GC55

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG35725.920867244608729No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA35425.871139916126573No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAGCGTCAGTTGCACCCCGGAT28814.775481111903065No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA24634.082613668385022No Hit
CCTGTTCGATACCCACGCTTTCGAGCTTCAGCGTCAGTTGCGCTACAGCA20653.422897777188417No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTCAGCGTCAGTCGCACTCCCGCA20473.393061380099123No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA18633.0880670987418988No Hit
CCTGTTCGATACCCACGCTTTCGAGCTTCAGCGTCAGTTACGCTACAGCA16422.721742445590015No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTACGCTACTGCA14712.4382966732417244No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA12632.093520529098775No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAATCGTCCAGTA10721.7769232044290473No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTCAGCGTCAGTCGCACTCCCACA10051.665865504152232No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA9981.654262460839729No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTA8151.350925757098576No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG7621.263074143446767No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGCA7071.171907374562814No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA6891.1420709774735203No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAGGCCCAGTA6521.0807406056788609No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGTA6381.0575345190538548No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA6000.9945465696431236No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGAA5410.8967494902948829No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA5400.8950919126788112No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAACAGTCCAGCA5310.8801737141341643No Hit
CCTATTTGCTCCCCACGCTTTCGTACTTCAGCGTCAGTTTCAGACCAGGC3500.5801521656251554No Hit
CCTGTTCGATACCCGCGCTTTCGAGCCTCAGCGTCAGTTGCGCTACCGTC3470.5751794327769397No Hit
CCTATTTGCTCCCCACGCTTTCGTGCTTCAGTGTCAGAATCCAGACCAGA3450.5718642775447961No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTATCGTCCAGCA3280.5436854580715742No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA2910.48235508627691487No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTGTCGTCCAGCA2840.47075204296441175No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA2760.4574914220358368No Hit
CCTGTTCGATACCCACGCTTTCGAGCTTCAGCGTCAGTTGCACTACAGCA2700.4475459563394056No Hit
CCTGTTCGATACCCGCGCTTTCGAGCTTCAGCGTCAGTTACGCTCCCGCC2680.4442308011072618No Hit
CCTATTTGCTCCCCACGCTTTCGTGCTTCAGTGTCAGCAACAGACCAGAC2660.4409156458751181No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG2560.42433986971439935No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGCA2490.41273682640189624No Hit
CCCGTTTGCTACCCTGGCTTTCGCATCTCAGCGTCAGACACAGTCCAGAA2130.35306403222330884No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG1990.3298579455983026No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTACGCTACTGTA1980.3282003679822308No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGCGTCAGTTACAGTCCAGCA1900.3149397470536558No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTCCAGAG1900.3149397470536558No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTTGCGTCCCAGGG1850.3066518589732964No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG1780.29504881566079333No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA1700.28178819473221833No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAATGAACCCCTCGTG1690.28013061711614645No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAG1650.273500306651859No Hit
CCTGTTCGATCCCCACGCTTTCGTGCCTCAGCGTCAATCGCCCCTTCGTG1580.26189726333935587No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGCTGTCCAGCA1480.24532148717863714No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGTA1440.23869117671434964No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGTA1400.23206086625006217No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCACCGTCAGTTACCGTCCAGTA1390.23040328863399032No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTAAAGGCCCAGCA1290.21382751247327159No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCAGTCGTGGCCCAGAG1220.20222446916076847No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTGCCGTCCAGTA1210.2005668915446966No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTTCAGTCCAGAA1200.1989093139286247No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTT1180.19559415869648097No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAG1170.1939365810804091No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTCCAGTA1130.1873062706161216No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGCGTCAATACCGGTCCAGGT1100.18233353776790598No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAGCGTCAGTGCTGCCCCGGCA1050.17404564968754663No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCTCAGAG990.1641001839911154No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAGAGCCCAGCA930.15415471829468416No Hit
CCTGTTCGCTCCCCCGGCTTTCGCGCCTCAGCGTCAGTGACGGCCCAGAG910.1508395630625404No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGCG910.1508395630625404No Hit
CCTATTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTCTCTGTCTAGGT880.14586683021432478No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCATTGTCAGTCCAGTA870.1442092525982529No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTG740.12266074358931857No Hit
CCTGTTTGATACCCGCACTTTCGAGCATGAACGTCAGTTGCGGTCTGGCA740.12266074358931857No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCTCAGTA720.11934558835717482No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGATGCCGGCCCAGGA710.11768801074110294No Hit
CCTATTTGCTCCCCACGCTTTCGGGACTGAGCGTCAGTTATGCGCCAGAT700.11603043312503108No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTGAACGTCAGTTACTGTCCAGCA680.11271527789288734No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA670.11105770027681545No Hit
GCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG660.1094001226607436No Hit
CCTGTTTGCTACCCACGCTTTCGCACCTCAGCGTCAGTTACCGTCCAGGT660.1094001226607436No Hit
CCTGTTCGATACCCACGCTTTCGAGCCTCAGCGTCAGTCGCGAGTTGGTA640.10608496742859984No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCACGGCCCAGAG640.10608496742859984No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)