FastQCFastQC Report
Thu 15 Dec 2022
EGAF00007707637

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007707637
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88543
Sequences flagged as poor quality0
Sequence length245-251
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG2014222.748269202534363No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAGCGTCAGTTGCACCCCGGAT69197.814282326101442No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA61206.911895914979163No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA25592.8901211840574637No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTATCGTCCAGTA23772.684571338219848No Hit
CCTATTTGCTCCCCACGCTTTCGGGAATGAGCGTCAGTTGCGGCCCAGAC22952.5919609681171862No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA17551.9820877991484365No Hit
CCTGTTCGATACCCGCACCTTCGAGCTTCAGCGTCAGTCGCACTCCCGCA12031.3586618930914924No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA10511.1869938899743626No Hit
CCTATTTGCTCCCCACGCTTTCGTGCTTCAGTGTCAGCAACAGACCAGAC9531.076313203754108No Hit
CCTGTTCGATACCCACGCTTTCGAGCTTCAGCGTCAGTTGCGCTACAGCA6670.7533063031521408No Hit
CCTGTTTGATACCCGCACTTTCGAGCATGAACGTCAGTTGCGGTCTGGCA6480.7318478027624996No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTAACGGCCCAGAG5720.6460138012039348No Hit
CCTGTTCGATACCCGCGCTTTCGAGCTTCAGCGTCAGTTGCGCCCCAGTG5560.6279434850863422No Hit
CCTGTTCGCTCCCCCAGCTTTCGCGCCTCAGCGTCGGTCTCGGCCCAGAG5480.618908327027546No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAG5310.5997086161526037No Hit
CCTGTTTGCTACCCATGCTTTCGAGCCTCAGCGTCAGTTGGTGCCCAGTA5000.5646973786747682No Hit
CCTGTTCGATACCCGCACTTTCGAGCCTCAGCGTCAGTTGCGCTCCAGTG4560.5150040093513886No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGTA3900.44046395536631916No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACTGTCCAGTA3660.4133584811899303No Hit
CCTATTTGCTCCCCACGCTTTCGGGACTGAGCGTCAGTTATGCGCCAGAT3480.39302937555763867No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAGCAGTCCAGTA3210.3625357171092012No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTGAACGTCAGTTACTGTCCAGCA2980.33655963769016184No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTTGCGTCCCAGGG2870.32413629535931693No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTGACGGCCCAGAG2800.31623053205787016No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTTCAGTCCAGTA2660.3004190054549767No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGCGTCAGTTAAAGCCCAGCA2620.29590142642557854No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAGCGTCAGTTGCACCTCGGAT2450.2767017155506364No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGCA2310.2608901889477429No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTACAGTCCAGTA2290.2586313994330438No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTTGCCGTCCAGTA2200.248466846616898No Hit
CCTGTTCGATACACGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG2000.22587895146990727No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGTGTCAGTTACAGTCCAGTG2000.22587895146990727No Hit
CCTGTTCGATACCCGCACTTTCGCGCTTCAGCGTCAGTTGCGCTCCAGTG2000.22587895146990727No Hit
CCTGTTTGATCCCCACGCTTTCGAGCCTCAACGTCAGTTACCGTCCAGTA1980.2236201619552082No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTAACAGTCCAGCA1940.21910258292581006No Hit
CCTGTTTGATACCCGCACTTTCGAGACTCAGCGTCAGTTGCACCCCGGAT1670.18860892447737257No Hit
CCTATTTGCTCCCCACGCTTTCGGGAATGAGCGTCAGTTGCGGCTCAGAC1570.1773149769038772No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGTGTCAGTTACAGACCAGGA1560.17618558214652769No Hit
CCTGTTTGCTCCCCACGCTTTCGAGACTCAACGTCAGTTACCGTCCAGTA1510.17053860835978No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGATGCGCTCCAGTG1500.16940921360243047No Hit
CCTATTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG1410.15924466078628463No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCATTTGCGCTCCAGTG1410.15924466078628463No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAGCGTCAGTTGCACCCCTGAT1380.155856476514236No Hit
CCTGTTTGATACCCGCACTTTCGAGCCTCAGCGTCATTTGCACCCCGGAT1360.15359768699953694No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAATGTCAGTTGCAGCTTAGCA1350.15246829224218741No Hit
CCTATTTGCTCCCCACGCTTTCGGGACTGAGCGTCAGTTGCGGCCCAGAC1340.15133889748483786No Hit
CCTGTTTGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG1300.14682131845543972No Hit
CCTGTTCGATACCCGCACTTTCGATCTTCAGCGTCAGTTGCGCTCCAGTG1300.14682131845543972No Hit
CCTGTTCGATACCCGCACTCTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG1240.1400449499113425No Hit
CCTGTTCGATACCCGCACTTTCGAGATTCAGCGTCAGTTGCGCTCCAGTG1200.13552737088194436No Hit
CCTGTTCGATACCCGCACTTGCGAGCTTCAGCGTCAGTTGCGCTCCAGTG1170.13213918660989576No Hit
CCTGTTTGCTCCCCACACTTTCGAGCCTCAGCGTCAGTTAAAGCCCAGTT1160.13100979185254621No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCACCGTCCAGTA1150.1298803970951967No Hit
CCTATTTGCTCCCCACGCTTTCAGGAATGAGCGTCAGTTGCGGCCCAGAC1120.12649221282314807No Hit
CCTGTTCGAGACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGTG1070.1208452390364004No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTCATGTCCCAGAG1040.11745705476435178No Hit
CCTGTTTGCTCCCCACACTTTCGCGCCTCACCGTCAGTTATCGTCCAGCA1010.11406887049230317No Hit
CCTGTTTGATACACGCACTTTCGAGCCTCAGCGTCAGTTGCACCCCGGAT1000.11293947573495364No Hit
CCTGTTCGATACCCGCACTTTCGAGCCTCAGCGTCAGTTGCACCCCGGAT990.1118100809776041No Hit
CCTGTTTGCTCCCCACGCTTTCGATCCTCAACGTCAGTTACCGTCCAGTA960.10842189670555548No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGACAGTTGCGCTCCAGTG950.10729250194820596No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAGCGTCAGTTGGTGCTCAGTA940.1061631071908564No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCAGTTGCGCTCCAGAG930.10503371243350688No Hit
CCTGTTCGATACCCGCACTTTCGAGCTTCAGCGTCCGTTGCGCTCCAGTG920.10390431767615733No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA890.10051613340410874No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)