FastQCFastQC Report
Thu 19 Jan 2023
EGAF00007731250

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007731250
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences210979719
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT51520622.4419702635019624Clontech SMART CDS Primer II A (100% over 26bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11123910.5272502045563915Clontech SMART CDS Primer II A (100% over 24bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT4801800.2275953358341519Clontech SMART CDS Primer II A (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4391710.2081579225157656No Hit
GTTCTGGCGCACCTGCGAGGTGGACGCGGTCGTCATGGCGGCGACTAAGG2345370.11116566137809672No Hit
GGCGGCCTCTGAGTCCTGGTGGTAGTTCTGGCGCACCTGCGAGGTGGACG2264730.1073434930492063No Hit
GGTAAACGTAGGAGGCGTAGAGCTCCAGGTTGATCTGGCGGTTGATGGCG2235410.10595378601295795No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2206680.10459204374994925Clontech SMART CDS Primer II A (100% over 23bp)
GTCCTGGTGGTAGTTCTGGCGCACCTGCGAGGTGGACGCGGTCGTCATGG2178110.10323788515425977No Hit
GGCGCACCTGCGAGGTGGACGCGGTCGTCATGGCGGCGACTAAGGAGAGG2159280.10234538230662826No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)