FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007740237

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007740237
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9748849
Sequences flagged as poor quality0
Sequence length31
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAA170558817.49527559612422Illumina Single End Adapter 1 (95% over 24bp)
TAGCTTATCAGACTGATGTTGAATCTCGTAT7375937.5659495803043No Hit
TAGCTTATCAGACTGATGTTGACATCTCGTA1973122.0239517506117903No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTAT1811701.858373229496118No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGTA1649401.6918920377164526No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTAT1138161.1674814124210973No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTAT938490.9626674902852634No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTAT866730.889058800685086No Hit
TAGCTTATCAGACTGATGTTGATCTCGTATG844310.8660612139956215No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGTA762140.7817743407452511No Hit
CCCCCCACTGCTAAATTTGACTGGATCTCGT635390.6517589922666769No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTAT628680.6448761284537282No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAAA572750.5875052531842476Illumina Single End Adapter 1 (95% over 23bp)
TGAGGTAGTAGATTGTATAGTTATCTCGTAT452100.4637470536265358No Hit
GCGGCGGCGGCGACTCTGGACGCGATCTCGT451110.4627315491295434No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTATG419360.43016360187751396No Hit
TCTTTGGTTATCTAGCTGTATGAATCTCGTA410070.420634271799676No Hit
CCTGGGAATACCGGGTGCTGTAGGCTTATCT380460.3902614554805393No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTAT348650.35763196250141943No Hit
GCGGCGGCGGCGACTCTGGACGATCTCGTAT314040.32213033559141185No Hit
GCGGCGGCGGCGACTCTGGACGCGAGATCTC309060.3170220402429046No Hit
TGAGGTAGTAGGTTGTGTGGTTAATCTCGTA296210.3038409970243667No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTAT294900.30249724864955857No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGTA291870.29938918943149084No Hit
GTAGTGTTTCCTACTTTATGGATCTCGTATG290810.2983018815862262No Hit
CGGCGACTCTGGACGCGAGCCGGGATCTCGT285710.293070494783538No Hit
TCTTTGGTTATCTAGCTGTATGATCTCGTAT262160.2689137969005367No Hit
CTTCTCACTACTGCACTTGACTAGATCTCGT260640.26735463848091195No Hit
GCGGCGGCGGCGACTCTGGACGCGAATCTCG248210.2546044153520072No Hit
TGAGGTAGTAGGTTGTGTGGTATCTCGTATG245700.25202975243539005No Hit
TGTCAGTTTGTCAAATACCCCAATCTCGTAT211020.21645632217711036No Hit
TTCTCACTACTGCACTTGACTAGATCTCGTA189900.19479222624127218No Hit
GCGGCGACTCTGGACGCGAGCCGGGATCTCG189110.1939818741679146No Hit
CCCCCACTGCTAAATTTGACTGGATCTCGTA188530.19338693213937358No Hit
TAGCTTATCAGACTGATGTTGACAATCTCGT188370.19322281020046572No Hit
TGAGGTAGTAGGTTGTGTGGATCTCGTATGC182430.18712978321851123No Hit
TTCTCGTATGCCGTCTTCTGCTTGAAAAAAA180200.18484233369498285Illumina Paired End PCR Primer 2 (95% over 24bp)
GTAGTGTTTCCTACTTTATGGAATCTCGTAT175930.1804623294503792No Hit
TAGCTTATCAGACTGATGTTGAAATCTCGTA172720.17716963305104017No Hit
GCGGCGGCGGCGACTCTGGACGCATCTCGTA170430.17482063780042137No Hit
TGAGGTAGTAGGTTGTATAGTTAATCTCGTA169770.17414363480242642No Hit
CTCCCACTGCTTCACTTGACTAGATCTCGTA168660.1730050388512531No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC161270.1654246567979461No Hit
TGAGGTAGTAGGTTGTGTGGTTTATCTCGTA158210.16228582471633318No Hit
ACTCCCACTGCTTCACTTGACTAGATCTCGT156580.1606138324637093No Hit
CCCCACTGCTAAATTTGACTGGATCTCGTAT151380.1552798694492037No Hit
CGCGACCTCAGATCAGACGTGGCATCTCGTA141420.1450632787521891No Hit
GGGCGCGCGCCGCGGCTGGACGAGATCTCGT140350.14396571328574276No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTAT139090.14267325301684333No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTAT137560.14110383697603687No Hit
TAAAGTGCTTATAGTGCAGGTAGATCTCGTA131400.13478514232808406No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTAT128710.1320258422301956No Hit
TCCCACTGCTTCACTTGACTAGATCTCGTAT123040.12620977102014813No Hit
CGGCGGCGGCGACTCTGGACGCGATCTCGTA121050.12416850440498156No Hit
GCGGCGGCGGCGACTCTGGACGCGAGCATCT118660.12171693294254532No Hit
GCGGCGGCGGCGACTCTGGACATCTCGTATG117290.12031163884064673No Hit
CGGCGGCGGCGACTCTGGACGCGAGATCTCG115460.11843449416438802No Hit
TAGGTAGTTTCCTGTTGTTGGATCTCGTATG114770.11772671830284785No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTAT108180.11096694594408017No Hit
GGCGACTCTGGACGCGAGCCGGGATCTCGTA108150.11093617308053495No Hit
TGTAAACATCCTTGACTGGAAGCATCTCGTA104980.10768450716592287No Hit
GGGCGCGCGCCGCGGCTGGACGATCTCGTAT104700.10739729377283411No Hit
GGGCGCGCGCCGCGGCTGGACGAATCTCGTA104180.10686389747138354No Hit
GTACAGTACTGTGATAACTGAATCTCGTATG103120.10577658962611894No Hit
CGGCGACTCTGGACGCGAGCCGGATCTCGTA101850.10447387173603775No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCATCT100770.10336604864840968No Hit
TGAGGTAGTAGGTTGTATAGATCTCGTATGC100520.10310960811886613No Hit
CAAAGTGCTTACAGTGCAGGTAGATCTCGTA99980.10255569657505209No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG97590.10010412511261585No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)