FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007740238

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007740238
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10048908
Sequences flagged as poor quality0
Sequence length31
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAA116723711.61556061613859Illumina Single End Adapter 1 (95% over 24bp)
AAGCTGCCAGTTGAAGAACTGTATCTCGTAT5706365.67858716588907No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTAT2480922.4688453710592237No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGTA2384012.3724070316894137No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTAT2099842.089620086082985No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGTA2040642.030708212275403No Hit
TCCTGTACTGAGCTGCCCCGAGATCTCGTAT1742091.7336112540785524No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTAT1401841.3950172496354827No Hit
AAACCGTTACCATTACTGAGTTTATCTCGTA1342911.3363740617388475No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTAT1221531.2155848177732347No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTAT1162091.1564341120448112No Hit
TACAGTACTGTGATAACTGAATCTCGTATGC1045161.040073209944802No Hit
TCGTTTCCCGGCCAATGCACCAATCTCGTAT1041141.036072775270706No Hit
GTAGTGTTTCCTACTTTATGGATCTCGTATG955690.9510386601210798No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGTA922830.9183385896258578No Hit
TCCTGTACTGAGCTGCCCCGAGTATCTCGTA757550.7538630068063117No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTATG600700.5977763952063249No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTAT560330.5576028758547694No Hit
TGAGGTAGTAGGTTGTGTGGATCTCGTATGC546110.5434520845449078No Hit
TCCTGTACTGAGCTGCCCCGAGAATCTCGTA542340.5397004331216884No Hit
TACAGTACTGTGATAACTGAATATCTCGTAT541180.538546078837621No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTAT498300.49587477564726434No Hit
TCCTGTACTGAGCTGCCCCGAATCTCGTATG463970.4617118596368879No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTAT444950.4427844299101952No Hit
GTACAGTACTGTGATAACTGAATCTCGTATG439890.4377490569124526No Hit
TAGCTTATCAGACTGATGTTGATCTCGTATG430650.42855402796005293No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAAA417890.4158561308353107Illumina Single End Adapter 1 (95% over 23bp)
TCGTATCCGGCTCGAAGGACCAATCTCGTAT377240.37540397424277344No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTAT366540.36475605110525444No Hit
GTGATCGTATAGTGGTTAGTACTCTGCATCT348470.3467739977318929No Hit
GGATATCATCATATACTGTAAGATCTCGTAT342520.34085295636102947No Hit
TGAGGTAGTAGGTTGTATAGATCTCGTATGC338380.33673310572651277No Hit
TCGTATCCCACTCCTGACACCAATCTCGTAT335670.3340362952870103No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTAT323850.322273823185564No Hit
CTGGGTCGGTCGGGCTGGGGCGATCTCGTAT320250.31869134437294083No Hit
TCAGTGCACTACAGAACTTTGATCTCGTATG296770.29532562145060937No Hit
AAACCGTTACCATTACTGAGTTATCTCGTAT281600.28022945378741654No Hit
CATTGCACTTGTCTCGGTCTGATCTCGTATG281590.2802195024573814No Hit
GTAGTGTTTCCTACTTTATGGAATCTCGTAT275510.2741690937960622No Hit
CCCCCCACTGCTAAATTTGACTGGCTTATCT270230.26891479153754816No Hit
TGTAAACATCCTTGACTGGATCTCGTATGCC257300.25604772180220975No Hit
GGCGGCGACTCTGGACGCGAGCATCTCGTAT249960.2487434455564724No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCGATCTC246970.24576799787598813No Hit
TACCCTGTAGATCCGAATTTGTATCTCGTAT243250.24206610310294413No Hit
TGAGGTAGTAGGTTGTGTGGTATCTCGTATG234980.23383635316394577No Hit
CAAAGTGCTGTTCGTGCAGGTAGAATCTCGT234790.23364727789327955No Hit
TACAGTACTGTGATAACTGAAGATCTCGTAT232500.23136842331524976No Hit
CGGCGGCGACTCTGGACGCGAGCATCTCGTA230060.22894029878669403No Hit
AAACCGTTACCATTACTGAGTTTAGATCTCG225150.22405419573947738No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTAT224130.2230391600759008No Hit
CGCGACCTCAGATCAGACGTGGCATCTCGTA223820.2227306688448138No Hit
TAGCAGCACGTAAATATTGGCGATCTCGTAT219730.2186605748604724No Hit
CATTGCACTTGTCTCGGTCTGTATCTCGTAT212740.21170459516596232No Hit
TTCCGGCTCGAAGGACCAATCTCGTATGCCG200600.199623680503394No Hit
TAGCACCATCTGAAATCGGTTATCTCGTATG197370.19640940090206818No Hit
TTTGGCAATGGTAGAACTCACACTATCTCGT197030.19607105568087596No Hit
TACAGTACTGTGATAACTGAAATCTCGTATG197000.19604120169077077No Hit
CTGGGTCGGTCGGGCTGGGGCATCTCGTATG196160.19520528996782538No Hit
TCTCCCAACCCTTGTACCAGATCTCGTATGC192900.19196115637639433No Hit
CTTTCAGTCGGATGTTTACAGATCTCGTATG188870.18795077037226335No Hit
TCGTATCCCAGCGGTGCCTCCAATCTCGTAT185880.18497532269177905No Hit
CTAAGCCAGGGATTGTGGGTATCTCGTATGC185390.18448770752006088No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCATCT183520.18262680880350382No Hit
TCTCCCAACCCTTGTACCAGTGATCTCGTAT182400.1815122598395766No Hit
TAGCTTATCAGACTGATGTTGACATCTCGTA180480.1796016044728442No Hit
TATTGCACTTGTCCCGGCCTGTTATCTCGTA176730.17586985570969504No Hit
TGAGGTAGTAGATTGTATAGTATCTCGTATG166280.16547071582305262No Hit
TGGTTAGTACTCTGCGATCTCGTATGCCGTC163620.16282366203372545No Hit
CGCGACCTCAGATCAGACGTGGCGATCTCGT158890.15811668292713996No Hit
CAAAGTGCTTACAGTGCAGGTAGATCTCGTA158390.15761911642538673No Hit
TCGTTGCCCGCATCCTCCACCAATCTCGTAT157960.15719120923387894No Hit
TGTAGTGTTTCCTACTTTATGGATCTCGTAT153560.15281262401845055No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCG145510.14480180334022363No Hit
TCCGGCTCGAAGGACCAATCTCGTATGCCGT144720.14401564826745356No Hit
TCGGATCCGGCTCGAAGGACCAATCTCGTAT144170.143468325115525No Hit
TCGGATCCCACTCCTGACACCAATCTCGTAT141940.1412491785177056No Hit
TCCCCGGCATCTCCACCAATCTCGTATGCCG140500.13981618699265633No Hit
AAACCGTTACCATTACTGAGTTTAATCTCGT137680.13700991192276812No Hit
TAAAGTGCTTATAGTGCAGGTAGATCTCGTA137320.1366516640415058No Hit
AAACCGTTACCATTACTGAGTTTAGTATCTC136100.13543760177722794No Hit
GCGGCGACTCTGGACGCGAGCATCTCGTATG134710.13405436690235398No Hit
CGCGACCTCAGATCAGAATCTCGTATGCCGT133700.13304928256881246No Hit
AACTCGTATGCCGTCTTCTGCTTGAAAAAAA133190.13254176473702417Illumina Single End Adapter 1 (95% over 24bp)
ATCCGGCTCGAAGGACCAATCTCGTATGCCG132700.132054149565306No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC131050.13041218010952035No Hit
AAGCTGCCAGTTGAAGAACTGTAATCTCGTA130670.1300340295681879No Hit
TGTAAACATCCTTGACTGGAAGATCTCGTAT129770.1291384098650321No Hit
TCGTTTCCCGGCCAATGCACCATCTCGTATG129690.12905879922475158No Hit
CGGCGACTCTGGACGCGAGCATCTCGTATGC129640.12900904257457627No Hit
CGCGACCTCAGATCAGACGTATCTCGTATGC128300.12767556434987762No Hit
CCCCCCACTGCTAAATTTGACTGGATCTCGT127280.12666052868630104No Hit
CGGCGGCGGCGACTCTGGACGCGAGCATCTC126390.1257748603131803No Hit
TGTAAACATCCTTGACTGGAAGCATCTCGTA126320.12570520100293484No Hit
TCTCCCAACCCTTGTACCAGTATCTCGTATG125190.12458070070897255No Hit
CGCGACCTCAGATCAGACGTGGATCTCGTAT125110.12450109006869203No Hit
CATTGCACTTGTCTCGGTCTGAAATCTCGTA124760.12415279351746479No Hit
CATGCCTTGAGTGTAGGACCGTATCTCGTAT123060.12246106741150382No Hit
TTTGGCAATGGTAGAACTCACAATCTCGTAT122940.12234165145108304No Hit
GTCGCTATCTCGTATGCCGTCTTCTGCTTGA122550.12195354957971552RNA PCR Primer, Index 31 (96% over 28bp)
TTCAAGTAATTCAGGATAGGTTATCTCGTAT120290.11970454899179095No Hit
TAGCACCATTTGAAATCGGTTATCTCGTATG118910.11833126544695204No Hit
CCCCGGCATCTCCACCAATCTCGTATGCCGT117990.11741574308372611No Hit
TGACCTATGAATTGACAGCCAGATCTCGTAT114990.11443034407320675No Hit
AAGCTGCCAGTTGAAGAACTGTTATCTCGTA114360.11380341028099769No Hit
GTTTGTGATGACTTACATGGAATCTCGTATG113750.11319637914885877No Hit
TGGAGAGAAAGGCAGTTCCTGAATCTCGTAT111410.11086776792065367No Hit
TTCTCACTACTGCACTTGACTAGATCTCGTA110740.11020102880830433No Hit
TCCCCGGCACCTCCACCAATCTCGTATGCCG110630.11009156417791863No Hit
CCGCACTGTGGGTACTTGCTGATCTCGTATG108600.10807144418080053No Hit
GTAAACATCCTTGACTGGAAGCTATCTCGTA106270.1057527842826305No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCT106160.10564331965224479No Hit
TTCTCGTATGCCGTCTTCTGCTTGAAAAAAA105140.10462828398866823Illumina Paired End PCR Primer 2 (95% over 24bp)
CTCGTACGACTCTTAGCATCTCGTATGCCGT105130.10461833265863316No Hit
AGGGCTGGGTCGGTCGGGCTGGGGCATCTCG103460.10295646054277738No Hit
TATTGCACTTGTCCCGGCCTGTAGATCTCGT102740.10223996478025274No Hit
TATTGCACTTGTCCCGGCCTGTAAATCTCGT102650.10215040280993717No Hit
TGTAAACATCCTTGACTGGAAGCTATCTCGT101710.1012149777866411No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)