FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007740239

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007740239
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13596999
Sequences flagged as poor quality0
Sequence length31
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAA212957015.662058958745234Illumina Single End Adapter 1 (95% over 24bp)
TACCCTGTAGATCCGAATTTGTATCTCGTAT154968611.397264940594612No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTAT5825784.284607213694728No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGTA4988723.6689860755303427No Hit
ACCCTGTAGATCCGAATTTGTATCTCGTATG2840032.0887182531969004No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGTA2763342.032316101516224No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTAT1608601.183055172689209No Hit
CACCCGTAGAACCGACCTTGCGATCTCGTAT1570911.1553358207939854No Hit
ACCCTGTAGATCCGAATTTGTGAATCTCGTA1535201.1290726725801774No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGTA1403621.0323013188424888No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTAT1236250.9092079803786115No Hit
TATTGCACTTGTCCCGGCCTGTAAATCTCGT1146870.8434728869215921No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTAT1117090.8215709951879823No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTAT1116170.8208943752956076No Hit
AACATTCAACGCTGTCGGTGAGTATCTCGTA993880.7309554115580945No Hit
TATTGCACTTGTCCCGGCCTGTATATCTCGT961000.7067736049697437No Hit
AACATTCAACGCTGTCGGTGAGATCTCGTAT905320.6658233923529744No Hit
TACCCTGTAGATCCGAATTTGTGAATCTCGT818480.601956358164033No Hit
TATTGCACTTGTCCCGGCCTGTAGATCTCGT781130.5744870614464265No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAAA757430.5570567446537283Illumina Single End Adapter 1 (95% over 23bp)
ACCCTGTAGATCCGAATTTGTGTATCTCGTA741290.5451864782809795No Hit
TACCCTGTAGATCCGAATTTGTGTATCTCGT684780.5036258368482633No Hit
GTTTGTGATGACTTACATGGAATCTCGTATG644030.47365598835448913No Hit
AACATTCAACGCTGTCGGTGAGAATCTCGTA596070.43838349918242986No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTAT582590.4284695468463298No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCATCT549690.4042730311298839No Hit
TACCCTGTAGATCCGAATTTGATCTCGTATG533480.39235128280880216No Hit
CACCCGTAGAACCGACCTTGCATCTCGTATG518430.3812826639172364No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTATG467650.34393618768376755No Hit
TCGTACCGTGAGTAATAATGCGATCTCGTAT454460.33423551770504656No Hit
TATTGCACTTGTCCCGGCCTGTTATCTCGTA444340.326792698888924No Hit
GTAGTGTTTCCTACTTTATGGATCTCGTATG437050.32143122169825855No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTAT384470.28276092393623037No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTAT334620.24609842215918382No Hit
CGCGACCTCAGATCAGAATCTCGTATGCCGT328400.2415238833216065No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTAT324620.23874385811163182No Hit
ACCATCGACCGTTGATTGTACCATCTCGTAT321850.23670664387045992No Hit
TACCCTGTAGATCCGAATTTGTGATCTCGTA309180.2273884112222116No Hit
TACAGTACTGTGATAACTGAATCTCGTATGC308430.22683681891864524No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTAT301340.22162243300893084No Hit
ACCCTGTAGATCCGAATTTGTGATCTCGTAT284860.20950211145856523No Hit
CACTAGATTGTGAGCTCCTGGAATCTCGTAT279500.20556006512907737No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTAT267960.19707289821820242No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTAT257410.1893138331480351No Hit
TATTGCACTTGTCCCGGCCTGTGAATCTCGT252190.18547475071521297No Hit
TGAGGTAGTAGGTTGTGTGGATCTCGTATGC240920.1771861570336219No Hit
CAAAGTGCTTACAGTGCAGGTAGATCTCGTA234050.1721335715329537No Hit
TATTGCACTTGTCCCGGCCTGTAATATCTCG229300.16864015361036652No Hit
TGTCAGTTTGTCAAATACCCCAATCTCGTAT223640.1644774703594521No Hit
AACTCGTATGCCGTCTTCTGCTTGAAAAAAA222020.1632860309837487Illumina Single End Adapter 1 (95% over 24bp)
TTCTCGTATGCCGTCTTCTGCTTGAAAAAAA218560.16074135182329571Illumina Paired End PCR Primer 2 (95% over 24bp)
CAAAGTGCTGTTCGTGCAGGTAGAATCTCGT216130.1589541927597406No Hit
TTGTAGTGTGCTGATGATCTCGTATGCCGTC211860.1558137939114359No Hit
TGAGGTAGTAGGTTGTATAGATCTCGTATGC207630.15270281331932142No Hit
TAGGTAGTTTCCTGTTGTTGGATCTCGTATG198740.1461646058810477No Hit
CATTGCACTTGTCTCGGTCTGATCTCGTATG195860.14404649143535275No Hit
CGCGACCTCAGATCAGACGTGGCGATCTCGT194900.14334045328678777No Hit
TGTAAACATCCTTGACTGGAAGCATCTCGTA186130.1368905006170847No Hit
CATTGCACTTGTCTCGGTCTGTATCTCGTAT182300.1340737025868723No Hit
TAAAGTGCTTATAGTGCAGGTAGATCTCGTA172650.12697654828098465No Hit
CTTTCAGTCGGATGTTTACAGATCTCGTATG166350.12234317293102692No Hit
CTCGTACGACTCTTAGCATCTCGTATGCCGT156690.11523866406109172No Hit
CACCCGTAGAACCGACCTTGCGAATCTCGTA152050.11182614634302761No Hit
TGTAAACATCCCCGACTGGAAGCATCTCGTA151770.11162021854969614No Hit
CTCGTACGACTCTTAGCGGATCTCGTATGCC145810.10723689837735517No Hit
CGCGACCTCAGATCAGACGTGGATCTCGTAT144170.10603074987355667No Hit
GTAGTGTTTCCTACTTTATGGAATCTCGTAT142470.10478047398547283No Hit
TACAGTACTGTGATAACTGAATATCTCGTAT140470.10330956117596242No Hit
ACCACTGACCGTTGACTGTACCATCTCGTAT139750.10278003256453869No Hit
TGTAAACATCCTTGACTGGATCTCGTATGCC137990.10148562929216956No Hit
TTCCGGCTCGAAGGACCAATCTCGTATGCCG136580.10044863576146473No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTAT136200.10016916232765774No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)