FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007740240

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007740240
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15707360
Sequences flagged as poor quality0
Sequence length31
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAA229449514.607769860753175Illumina Single End Adapter 1 (95% over 24bp)
AAGCTGCCAGTTGAAGAACTGTATCTCGTAT10075796.414693494005358No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTAT6232733.96803154699453No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGTA5450523.470042069450245No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTAT3442122.1914058123070967No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGTA2976871.895207087632804No Hit
TACAGTACTGTGATAACTGAATCTCGTATGC2453441.5619684020739322No Hit
TAGCTTATCAGACTGATGTTGACATCTCGTA2076701.322119057562824No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTAT1924231.2250499129070704No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGTA1760591.1208694522822422No Hit
TAGCTTATCAGACTGATGTTGATCTCGTATG1627001.0358201505536258No Hit
TTTGGCAATGGTAGAACTCACAATCTCGTAT1428370.9093635085717777No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTAT1425450.9075045074410976No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCATCT1320890.8409369875014006No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTATG1277780.8134912550549551No Hit
TGAGGTAGTAGGTTGTATAGATCTCGTATGC1256650.8000389626264376No Hit
GTAGTGTTTCCTACTTTATGGATCTCGTATG1184810.7543024416579235No Hit
TTTGGCAATGGTAGAACTCACACTATCTCGT1120020.7130542624604007No Hit
TCTCCCAACCCTTGTACCAGTGATCTCGTAT998280.6355491947723869No Hit
TGAGGTAGTAGGTTGTGTGGATCTCGTATGC929000.5914424830143321No Hit
CACCCGTAGAACCGACCTTGCGATCTCGTAT919130.5851588045349442No Hit
CCCCCCACTGCTAAATTTGACTGGATCTCGT896920.571018936345764No Hit
TCGTACCGTGAGTAATAATGCGATCTCGTAT831830.5295797638813906No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTAT827490.5268167279542838No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAAA762280.48530115818317016Illumina Single End Adapter 1 (95% over 23bp)
TCCTGTACTGAGCTGCCCCGAGATCTCGTAT714850.4551051226940746No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTAT691450.4402076478797201No Hit
GTACAGTACTGTGATAACTGAATCTCGTATG688020.43802395819539375No Hit
TCTCCCAACCCTTGTACCAGATCTCGTATGC648290.4127300832221328No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTAT615780.39203277953774535No Hit
TCTCCCAACCCTTGTACCAGTATCTCGTATG610110.3884230067942671No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTAT527470.33581072821912783No Hit
TATTGCACTTGTCCCGGCCTGTTATCTCGTA527300.3357024987012458No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTAT491270.3127642073524768No Hit
GTAGTGTTTCCTACTTTATGGAATCTCGTAT467820.2978349003269805No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTAT434700.27674924366666326No Hit
TACAGTACTGTGATAACTGAATATCTCGTAT433890.2762335618461664No Hit
TGTAAACATCCTTGACTGGATCTCGTATGCC420950.2679953856026729No Hit
GTTTGTGATGACTTACATGGAATCTCGTATG379980.2419120717930957No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTAT379820.24181020871744202No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTAT356510.2269700318831427No Hit
TCCTGTACTGAGCTGCCCCGAGAATCTCGTA352750.22457624960528058No Hit
TGAGGTAGTAGATTGTATAGTATCTCGTATG352370.22433432480060303No Hit
CTTTCAGTCGGATGTTTACAGATCTCGTATG328490.20913126075928737No Hit
AACATTCAACGCTGTCGGTGAGATCTCGTAT326890.2081126300027503No Hit
TGAGGTAGTAGGTTGTGTGGTATCTCGTATG317910.20239556488168606No Hit
CATTGCACTTGTCTCGGTCTGATCTCGTATG314420.20017367654398954No Hit
TCCTGTACTGAGCTGCCCCGAATCTCGTATG313000.1992696417475629No Hit
CACTAGATTGTGAGCTCCTGGAATCTCGTAT312160.19873486060038095No Hit
CCCCCACTGCTAAATTTGACTGGATCTCGTA298460.1900128347475324No Hit
CGCGGGTGATGCGAACTGGAGTCTGAGCATC283170.18027854458037507No Hit
TTTGGCAATGGTAGAACTCAATCTCGTATGC280510.1785850709476322No Hit
TATTGCACTTGTCCCGGCCTGTATATCTCGT275860.17562467531144635No Hit
TAGCTTATCAGACTGATGTTGACTATCTCGT271150.17262608102189037No Hit
CAAAGTGCTGTTCGTGCAGGTAGAATCTCGT260320.16573122408858013No Hit
TTATGGTTTGCCTGGGACTGAATCTCGTATG260220.16566755966629657No Hit
TGAGGTAGTAGGTTGTATAGAATCTCGTATG255340.16256073585885852No Hit
AACTCGTATGCCGTCTTCTGCTTGAAAAAAA254640.16211508490287355Illumina Single End Adapter 1 (95% over 24bp)
TCAGTGCACTACAGAACTTTGATCTCGTATG248920.15847347994825356No Hit
TACAGTACTGTGATAACTGAAATCTCGTATG248210.15802146255004024No Hit
TGTAGTGTTTCCTACTTTATGGATCTCGTAT240890.15336122683888317No Hit
CGCGACCTCAGATCAGAATCTCGTATGCCGT240570.15315750068757578No Hit
TAGCACCATCTGAAATCGGTTATCTCGTATG230740.14689928797710117No Hit
CATTGCACTTGTCTCGGTCTGTATCTCGTAT225790.14374789907406466No Hit
TCCTGTACTGAGCTGCCCCGAGTATCTCGTA222480.1416406066964786No Hit
TACAGTACTGTGATAACTGAAGATCTCGTAT220420.1403291195974371No Hit
ATGTGTGACTGAAAGGTATTTTCTGAGCATC220050.14009356123498792No Hit
TTCAAGTAATCCAGGATAGGATCTCGTATGC216680.13794807020403174No Hit
TTTGGCAATGGTAGAACTCACACTGATCTCG215180.13699310386977825No Hit
AACATTCAACGCTGTCGGTGAGAATCTCGTA213760.1360890690733516No Hit
TATTGCACTTGTCCCGGCCTGTAAATCTCGT213530.1359426409020994No Hit
CTAGACTGAAGCTCCTTGAGGATCTCGTATG208560.13277851911460614No Hit
TAAAGTGCTTATAGTGCAGGTAGATCTCGTA204230.13002184962972774No Hit
CACCCGTAGAACCGACCTTGCATCTCGTATG200870.1278827250409999No Hit
TCGTTTCCCGGCCAATGCACCAATCTCGTAT189490.12063771378513002No Hit
CAAAGTGCTTACAGTGCAGGTAGATCTCGTA183310.11670325248800562No Hit
TTGTAGTGTGCTGATGATCTCGTATGCCGTC183130.1165886565278952No Hit
GCGCAGTGATGACCCTCATCTATCACCCTTG180900.11516893991097166No Hit
CTTTCAGTCGGATGTTTACAATCTCGTATGC180320.11479968626172699No Hit
AAGCTGCCAGTTGAAGAACTGTAATCTCGTA178360.11355186358496909No Hit
TAGCAGCACGTAAATATTGGCGATCTCGTAT175710.11186475639445458No Hit
TGAGGTAGTAGATTGTATAGATCTCGTATGC172260.10966833382567154No Hit
AAGCTGCCAGTTGAAGAACTGTTATCTCGTA171310.10906352181397766No Hit
TGTCGGTGCTGAAATCCAGAGGCTGTTTCTG170610.10861787085799268No Hit
TGAGGTAGTAGGTTGTGTGAATCTCGTATGC170340.10844597691782706No Hit
CACCATGATGGAACTGAGGATCTGAGGATCT169130.10767563740819591No Hit
CTGGGTCGGTCGGGCTGGGGCGATCTCGTAT165900.10561927656843671No Hit
TTCTCGTATGCCGTCTTCTGCTTGAAAAAAA161230.10264614804779415Illumina Paired End PCR Primer 2 (95% over 24bp)
TTTGGCAATGGTAGAACTCACACTGGATCTC159710.10167844882908393No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)