FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007740241

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007740241
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10000425
Sequences flagged as poor quality0
Sequence length31
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAA195558019.554968913821163Illumina Single End Adapter 1 (95% over 24bp)
AGCAGCATTGTACAGGGCTATGAATCTCGTA3444013.4438636357954784No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTAT2765152.76503248611934No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTAT1623701.6236309956826833No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTAT1562671.5626035893474528No Hit
TCTCCCAACCCTTGTACCAGTGATCTCGTAT1361821.361762125109683No Hit
CATTGCACTTGTCTCGGTCTGAATCTCGTAT1215291.2152383523700243No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCATCT983530.9834882017514255No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGTA877970.877932687860766No Hit
TCTCCCAACCCTTGTACCAGATCTCGTATGC763370.7633375581537785No Hit
TAGCACCATCTGAAATCGGTTATCTCGTATG759840.7598077081724027No Hit
TGTCAGTTTGTCAAATACCCCAATCTCGTAT754730.7546979253381731No Hit
CCCCCCACTGCTAAATTTGACTGGCTTATCT695870.6958404267818618No Hit
GTTTGTGATGACTTACATGGAATCTCGTATG677460.6774312091736101No Hit
TCTCCCAACCCTTGTACCAGTATCTCGTATG644890.6448625933397831No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGTA609650.6096240909761335No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTAT590150.5901249196909131No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAAA573060.5730356459850456Illumina Single End Adapter 1 (95% over 23bp)
TAGCTTATCAGACTGATGTTGACATCTCGTA470930.4709099863255812No Hit
CACTAGATTGTGAGCTCCTGGAATCTCGTAT401150.4011329518495464No Hit
CTCGTACGACTCTTAGCATCTCGTATGCCGT352480.35246502023663995No Hit
TGTCAGTTTGTCAAATACCCCAAATCTCGTA343910.343895384446161No Hit
TCGTTTCCCGGCCAATGCACCAATCTCGTAT335420.3354057452558266No Hit
CACCCGTAGAACCGACCTTGCGATCTCGTAT322210.3221963066569671No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTAT298200.29818732703860085No Hit
CTCGTACGACTCTTAGCGGTGATCTCGTATG296000.2959874205346273No Hit
CTCGTACGACTCTTAGCGGATCTCGTATGCC291010.29099763260061445No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTAT287810.2877977685948347No Hit
TAGCTTATCAGACTGATGTTGATCTCGTATG272530.2725184179672364No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTAT258100.2580890312161733No Hit
GTAGTGTTTCCTACTTTATGGATCTCGTATG250650.2506393478277173No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTAT250230.25021936567695874No Hit
TATTGCACTTGTCCCGGCCTGTATATCTCGT248360.2483494451485812No Hit
TATTGCACTTGTCCCGGCCTGTAAATCTCGT228930.22892027088848724No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC214350.21434089051215321No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTAT212020.21201098953294484No Hit
ATGTGTGACTGAAAGGTATTTTCTGAGCATC201920.20191141876470248No Hit
TCGTACCGTGAGTAATAATGCGATCTCGTAT201230.2012214480884562No Hit
AGTCTGTGATGAATTGCTTTGACTTCTGACA200490.20048147953711964No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTAT189730.18972193681768523No Hit
TACAGTACTGTGATAACTGAATCTCGTATGC185080.18507213443428655No Hit
AATGTGTGACTGAAAGGTATTTTCTGAGCAT183470.1834622028563786No Hit
CGCGGGTGATGCGAACTGGAGTCTGAGCATC177390.17738246124539708No Hit
CTTTCAGTCGGATGTTTACAGATCTCGTATG177220.17721246847009003No Hit
CACCATGATGGAACTGAGGATCTGAGGATCT176810.1768024858943495No Hit
CGCGACCTCAGATCAGAATCTCGTATGCCGT176630.17662249354402437No Hit
CTCGTACGACTCTTAGCGATCTCGTATGCCG172580.17257266566170937No Hit
AACTCGTATGCCGTCTTCTGCTTGAAAAAAA163960.16395303199614017Illumina Single End Adapter 1 (95% over 24bp)
CTCGTACGACTCTTAGCGGTGGATCATCTCG163110.1631030681196049No Hit
TCTCCCAACCCTTGTACCAGTGAATCTCGTA160650.16064317266516173No Hit
TAGCACCATCTGAAATCGGTATCTCGTATGC158810.15880325086183839No Hit
TTCACCACCTTCTCCACCCAGCATCTCGTAT148840.14883367456883081No Hit
ACTCGGCGTGGCGTCGGTCGTGGATCTCGTA146530.14652377273965855No Hit
TGAGGTAGTAGGTTGTGTGGATCTCGTATGC145140.14513383181214798No Hit
TAGCACCATCTGAAATCGGTTAATCTCGTAT142990.1429839231832647No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG141040.14103400605474267No Hit
GCGCAGTGATGACCCTCATCTATCACCCTTG140640.1406340230540202No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAAC140190.14018404217820743No Hit
TGGCTCAGTTCAGCAGGAACAGTATCTCGTA139180.13917408510138318No Hit
TATTGCACTTGTCCCGGCCTGTTATCTCGTA138610.1386041093253537No Hit
TATTGCACTTGTCCCGGCCTGTAGATCTCGT138450.1384441161250647No Hit
TGTAAACATCCTTGACTGGATCTCGTATGCC137680.13767414884867393No Hit
TGAGGTAGTAGGTTGTATAGATCTCGTATGC135260.135254251694303No Hit
TGGCTCAGTTCAGCAGGAACAGATCTCGTAT134340.1343342907926413No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTATG134290.134284292917551No Hit
CGCGACCTCAGATCAGACGTGGATCTCGTAT134090.13408430141718977No Hit
GTCGCTATCTCGTATGCCGTCTTCTGCTTGA133160.13315434094051RNA PCR Primer, Index 31 (96% over 28bp)
ATGCAGTGTGGAACACAATGAACTGAACATC130540.13053445228577784No Hit
TCTCCCAACCCTTGTACCAGTGTATCTCGTA127920.12791456363104567No Hit
TGTCAGTTTGTCAAATACCCCATATCTCGTA126560.12655462142858928No Hit
CCCCCACTGCTAAATTTGACTGGCTTATCTC121450.12144483859435973No Hit
CTGTCTGAGCGTCGCTATCTCGTATGCCGTC120170.12016489299204783No Hit
CACCCGTAGAACCGACCTTGCATCTCGTATG119870.11986490574150599No Hit
GTAGTGTTTCCTACTTTATGGAATCTCGTAT118180.11817497756345355No Hit
GAGACTCTGAAATCTGATTATCTCGTATGCC117590.11758500263738791No Hit
CAACGGAATCCCAAAAGCAGCTGATCTCGTA113340.11333518325471166No Hit
CATTGCACTTGTCTCGGTCTGTATCTCGTAT112660.11265521215348348No Hit
CACTAGATTGTGAGCTCCTGGAAATCTCGTA112340.1123352257529055No Hit
AATGACACGATCACTCCCGTTGAATCTCGTA109410.10940535027261342No Hit
AAGCTGCCAGTTGAAGAACTGTAATCTCGTA107900.1078954144448861No Hit
TGAGATGAAGCACTGTAGCTATCTCGTATGC107350.10734543781889269No Hit
CGCGACCTCAGATCAGACGTGGCGATCTCGT105990.1059854956164363No Hit
AACATTCAACGCTGTCGGTGAGATCTCGTAT104770.10476554746423276No Hit
AACTTGACCGCTCTGACCAATCTCGTATGCC102730.10272563416054818Illumina PCR Primer Index 4 (95% over 21bp)
TCAGTGCACTACAGAACTTTGATCTCGTATG102460.10245564563506052No Hit
TTCTCGTATGCCGTCTTCTGCTTGAAAAAAA102390.10238564860993407Illumina Paired End PCR Primer 2 (95% over 24bp)
CAAAGTGCTGTTCGTGCAGGTAGAATCTCGT101200.10119569918278473No Hit
TTTCCGGCTCGAAGGACCAATCTCGTATGCC101190.10118569960776667No Hit
CCTACTGTGGAATCTGAAGACAATCTCGTAT100750.10074571830697196No Hit
TGTCGCTGATGCCATCACCGCAGCGCTCTGA100490.10048572935650235No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)