FastQCFastQC Report
Wed 4 Jan 2023
EGAF00007740242

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007740242
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7360405
Sequences flagged as poor quality0
Sequence length31
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTATGCCGTCTTCTGCTTGAAAAAAA162775122.114965141184488Illumina Single End Adapter 1 (95% over 24bp)
CATTGCACTTGTCTCGGTCTGAATCTCGTAT2412003.276993589347325No Hit
TATTGCACTTGTCCCGGCCTGTATCTCGTAT2409463.2735426922839164No Hit
AGCAGCATTGTACAGGGCTATGAATCTCGTA2181382.963668439440493No Hit
AAGCTGCCAGTTGAAGAACTGTATCTCGTAT2002572.7207334379018544No Hit
TATTGCACTTGTCCCGGCCTGTAATCTCGTA1524622.0713805829978105No Hit
TCCTGTACTGAGCTGCCCCGAGATCTCGTAT1054511.4326793158800364No Hit
CAAAGTGCTGTTCGTGCAGGTAGATCTCGTA860521.1691204492144114No Hit
TCTCCCAACCCTTGTACCAGTGATCTCGTAT737761.0023361486222566No Hit
TCGTTTCCGGCTCGAAGGACCAATCTCGTAT651540.8851958553911096No Hit
GCGGGTGATGCGAACTGGAGTCTGAGCATCT642390.8727644742374909No Hit
TCTCCCAACCCTTGTACCAGATCTCGTATGC617940.8395461934499528No Hit
TAGCTTATCAGACTGATGTTGAATCTCGTAT516350.701523897122509No Hit
AGCAGCATTGTACAGGGCTATGATCTCGTAT468520.6365410599009158No Hit
TCTCGTATGCCGTCTTCTGCTTGAAAAAAAA440970.5991110543509495Illumina Single End Adapter 1 (95% over 23bp)
TCCTGTACTGAGCTGCCCCGAATCTCGTATG380770.5173220767063769No Hit
TCTCCCAACCCTTGTACCAGTATCTCGTATG372200.5056786956695998No Hit
TGTAAACATCCCCGACTGGAAGATCTCGTAT349610.47498744973951845No Hit
TCCTGTACTGAGCTGCCCCGAGAATCTCGTA338160.45943124053635637No Hit
TATTGCACTTGTCCCGGCCTGTAAATCTCGT269750.36648798537580474No Hit
TTTGGCAATGGTAGAACTCACAATCTCGTAT256020.34783412054092133No Hit
TGAGGTAGTAGGTTGTATAGTATCTCGTATG239580.32549839309114104No Hit
TGAGGTAGTAGGTTGTGTGGATCTCGTATGC235210.3195612197970084No Hit
CCCCCCACTGCTAAATTTGACTGGATCTCGT228640.310635080542443No Hit
TGAGGTAGTAGGTTGTATAGTTATCTCGTAT218720.2971575613026729No Hit
TGAGGTAGTAGGTTGTATAGATCTCGTATGC217300.29522831963730256No Hit
TGTCAGTTTGTCAAATACCCCAATCTCGTAT211300.2870765942906674No Hit
TATTGCACTTGTCCCGGCCTGTATATCTCGT203240.27612610990835423No Hit
TCGTTTCCCGGCCAATGCACCAATCTCGTAT203240.27612610990835423No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCC202800.27552831671626765No Hit
CGCGACCTCAGATCAGACGTGGATCTCGTAT201790.2741561096162507No Hit
TAGCACCATCTGAAATCGGTTATCTCGTATG193980.263545280456714No Hit
TTTGGCAATGGTAGAACTCACACTATCTCGT193480.2628659700111611No Hit
AAACCGTTACCATTACTGAGTATCTCGTATG189260.25713258985069437No Hit
CATTGCACTTGTCTCGGTCTGATCTCGTATG180170.24478272595054212No Hit
TATTGCACTTGTCCCGGCCTGTAGATCTCGT179410.24375017407330168No Hit
TATTGCACTTGTCCCGGCCTGTTATCTCGTA174930.23766355248114746No Hit
CGCGACCTCAGATCAGACGTGGCGATCTCGT162010.22011017056805976No Hit
TACAGTACTGTGATAACTGAATCTCGTATGC154250.20956727245307832No Hit
AACATTCAACGCTGTCGGTGAGATCTCGTAT146920.1996085813212724No Hit
CACTAGATTGTGAGCTCCTGGAATCTCGTAT144380.19615768425786353No Hit
CAAAGTGCTGTTCGTGCAGGTAGAATCTCGT141790.19263885614989937No Hit
CTCGTACGACTCTTAGCATCTCGTATGCCGT141310.19198671812216855No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCG137230.18644354488645667No Hit
GTTTGTGATGACTTACATGGAATCTCGTATG134840.18319644095671367No Hit
CTTTCAGTCGGATGTTTACAGTATCTCGTAT134120.18221823391511743No Hit
CGCGACCTCAGATCAGAATCTCGTATGCCGT125880.17102319777240518No Hit
CATTGCACTTGTCTCGGTCTGAAATCTCGTA122380.16626802465353469No Hit
AAACCGTTACCATTACTGAGTTATCTCGTAT121740.16539850728322694No Hit
TCCTGTACTGAGCTGCCCCGAGTATCTCGTA120470.16367305875152252No Hit
AAACCGTTACCATTACTGAGTTTATCTCGTA119800.16276278275448158No Hit
AACTCGTATGCCGTCTTCTGCTTGAAAAAAA119460.1623008516515056Illumina Single End Adapter 1 (95% over 24bp)
TCAGTGCACTACAGAACTTTGATCTCGTATG115030.15628216110390664No Hit
CGCGGGTGATGCGAACTGGAGTCTGAGCATC114140.15507298851082243No Hit
TGTCAGTTTGTCAAATACCCCAAATCTCGTA112940.1534426434414954No Hit
TCAGTGCACTACAGAACTTTGTATCTCGTAT110650.150331401600863No Hit
CTCGTACGACTCTTAGCGGATCTCGTATGCC109350.14856519444242539No Hit
AATGTGTGACTGAAAGGTATTTTCTGAGCAT107960.14667671140378824No Hit
TGAGGTAGTAGGTTGTGTGGTATCTCGTATG107910.14660878035923294No Hit
CATTGCACTTGTCTCGGTCTGTATCTCGTAT102170.13881029644428533No Hit
CGCGACCTCAGATCAGACGATCTCGTATGCC99590.13530505454523223No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCT97850.13294105419470803No Hit
TACCCTGTAGATCCGAATTTGTATCTCGTAT97580.13257422655410944No Hit
TGAGGTAGTAGGTTGTGTGGTTATCTCGTAT95540.12980263993625352No Hit
AACATTCAACGCTGTCGGTGAGAATCTCGTA93930.12761526030157308No Hit
TAGCTTATCAGACTGATGTTGATCTCGTATG93570.12712615678077496No Hit
TCGTTTCCCGGCCAATGCACCATCTCGTATG92740.1259985014411571No Hit
TCGTTTCCGGCTCGAAGGACCATCTCGTATG92020.1250202943995609No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCA91980.12496594956391666No Hit
CCCCCACTGCTAAATTTGACTGGATCTCGTA88850.12071346617475534No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGGCGC87800.11928691423909417No Hit
GTGATCGTATAGTGGTTAGTACTCTGCATCT87210.11848532791334171No Hit
TTCTCGTATGCCGTCTTCTGCTTGAAAAAAA84660.11502084464102179Illumina Paired End PCR Primer 2 (95% over 24bp)
AACATTCAACGCTGTCGGTGAGTATCTCGTA83790.11383884446575969No Hit
TGTAAACATCCTTGACTGGATCTCGTATGCC83340.11322746506476206No Hit
TAGCTTATCAGACTGATGTTGACATCTCGTA83050.11283346500634137No Hit
CTTTCAGTCGGATGTTTACAGCATCTCGTAT82980.11273836154396397No Hit
ATGTGTGACTGAAAGGTATTTTCTGAGCATC82310.11182808554692303No Hit
GGATATCATCATATACTGTAAGATCTCGTAT82060.11148843032414656No Hit
CTGGGTCGGTCGGGCTGGGGCGATCTCGTAT79510.10802394705182662No Hit
CACCATGATGGAACTGAGGATCTGAGGATCT79170.10756201594885063No Hit
CGGCGGCGACTCTGGACGCGAGCATCTCGTA79130.10750767111320642No Hit
CGCGACCTCAGATCAGACGTGGCATCTCGTA79070.10742615385974005No Hit
TCTCCCAACCCTTGTACCAGTGAATCTCGTA78910.10720877451716312No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCG78060.10605394675972314No Hit
TGAGGTAGTAGATTGTATAGTTATCTCGTAT77420.1051844293894154No Hit
CGGCGGCGGCGACTCTGGACGCGAGCATCTC75900.10311932563493449No Hit
CTTTCAGTCGGATGTTTACAGATCTCGTATG74280.100918359791343No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGAT73910.10041567006163384No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)